Glycan profiling of monoclonal antibodies using zwitterionic-type hydrophilic interaction chromatography coupled with electrospray ionization mass spectrometry detection

Analytical Biochemistry

Volumn 408, Issue 2, 2011, Pages 235-241

  Mauko, Lea ^a   Nordborg, Anna ^a   Hutchinson, Joseph P ^a   Lacher, Nathan A ^b   Hilder, Emily F ^a   Haddad, Paul R ^a  

^a UNIVERSITY OF TASMANIA   (Australia)

^b PFIZER INC   (United States)

Author keywords

ESI MS; Glycans; Hydrophilic interaction chromatography; Liquid chromatography; Monoclonal antibodies; ZIC HILIC

Indexed keywords

AMIDES; CHEMICAL DETECTION; COLUMN CHROMATOGRAPHY; ELECTRODEPOSITION; ELECTROSPRAY IONIZATION; FLUORESCENCE; GLYCOSYLATION; HYDROPHILICITY; MASS SPECTROMETRY; MONOCLONAL ANTIBODIES; POLYSACCHARIDES;

ELECTROSPRAY IONIZATION MASS SPECTROMETRY; ESI-MS; FLUORESCENCE DETECTION; GLYCANS; HYDROPHILIC INTERACTION CHROMATOGRAPHY; MONOCLONAL ANTIBODIES (MABS); PHARMACEUTICAL INDUSTRY; ZIC-HILIC;

LIQUID CHROMATOGRAPHY;

ALDITOL; AMPHOLYTE; FLUORESCENT DYE; GLYCAN DERIVATIVE; MONOCLONAL ANTIBODY; RIBONUCLEASE;

ANALYTIC METHOD; ARTICLE; CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; FLUORESCENCE; GLYCOSYLATION; INTERMETHOD COMPARISON; PRIORITY JOURNAL; ZWITTERIONIC TYPE HYDROPHILIC INTERACTION CHROMATOGRAPHY COLUMN;

EID: 78149407007     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.09.035     Document Type: Article

References (26)

1. Jefferis R. Glycosylation of recombinant antibody therapeutics. Biotechnol. Progr. 2008, 21:11-16.
2. Mori K., Iida S., Yamane-Ohnuki N., Kanda Y., Kuni-Kamochi R., Nakano R., Imai-Nishiya H., Okazaki A., Shinkawa T., Natsume A., Niwa R., Shitara K., Satoh M. Non-fucosylated therapeutic antibodies: the next generation of therapeutic antibodies. Cytotechnology 2007, 55:109-114.
3. Kaneko Y., Nimmerjahn F., Ravetch E.V. Anti-inflammatory activity of immunoglobulin G resulting from Fc sialylation. Science 2006, 313:670-673.
4. Huhn C., Selman M.H.J., Ruhaak L.R., Deelder A.M., Wuhrer M. IgG glycosylation analysis. Proteomics 2009, 9:882-913.
5. Krenkova J., Lacher N.A., Svec F. Multidimensional system enabling deglycosylation of proteins using a capillary reactor with peptide-N-glycosidase F immobilized on a porous polymer monolith and hydrophilic interaction liquid chromatography-mass spectrometry of glycans. J. Chromatogr. A 2009, 1216:3252-3259.
6. Palm A.K., Novotny M.V. A monolithic PNGase F enzyme microreactor enabling glycan mass mapping of glycoproteins by mass spectrometry. Rapid Commun. Mass Spectrom. 2005, 19:1730-1738.
7. Wuhrer M., Deelder A.M., Hokke C.H. Protein glycosylation analysis by liquid chromatography-mass spectrometry. J. Chromatogr. B 2005, 825:124-133.
8. Bruggink C., Wuhrer M., Koeleman C.A.M., Barreto V., Liu Y., Pohl C., Ingendoh A., Hokke C.H., Deelder A.M. Oligosaccharide analysis by capillary-scale high-pH anion-exchange chromatography with on-line ion-trap mass spectrometry. J. Chromatogr. B 2005, 829:136-143.
9. Kamoda S., Kakehi K. Evaluation of glycosylation for quality assurance of antibody pharmaceuticals by capillary electrophoresis. Electrophoresis 2008, 29:3595-3604.
10. Deguchi K., Keira T., Yamada K., Ito H., Takegawa Y., Nakagawa H., Nishimura S.I. Two-dimensional hydrophilic interaction chromatography coupling anion-exchange and hydrophilic interaction columns for separation of 2-pyridylamino derivatives of neutral and sialylated N-glycans. J. Chromatogr. A 2008, 1189:169-174.
11. Anumula K.R. Advances in fluorescence derivatization methods for high-performance liquid chromatographic analysis of glycoprotein carbohydrates. Anal. Biochem. 2006, 350:1-23.
12. Bigge J.C., Patel T.P., Bruce J.A., Goulding P.N., Charles S.M., Parekh R.B. Nonselective and efficient fluorescent labeling of glycans using 2-amino benzamide and anthranilic acid. Anal. Biochem. 1995, 230:229-238.
13. Morelle W., Canis K., Chirat F., Faid V., Michalski J.C. The use of mass spectrometry for the proteomic analysis of glycosylation. Proteomics 2006, 6:3993-4015.
14. Wuhrer M., de Boer A.R., Deelder A.M. Structural glycomics using hydrophilic interaction chromatography (HILIC) with mass spectrometry. Mass Spectrom. Rev. 2009, 28:192-206.
15. Prater B.D., Connelly H.M., Qin Q., Cockrill S.L. High-throughput immunoglobulin G N-glycan characterization using rapid resolution reverse-phase chromatography tandem mass spectrometry. Anal. Biochem. 2009, 385:69-79.
16. Kawasaki N., Ohta M., Hyuga S., Hashimoto O., Hayakawa T. Analysis of carbohydrate heterogeneity in a glycoprotein using liquid chromatography mass spectrometry and liquid chromatography with tandem mass spectrometry. Anal. Biochem. 1999, 269:297-303.
17. Stadlmann J., Pabst M., Kolarich D., Kunert R., Altmann F. Analysis of immunoglobulin glycosylation by LC-ESI-MS of glycopeptides and oligosaccharides. Proteomics 2008, 8:2858-2871.
18. Ruhaak L.R., Deelder A.M., Wuhrer M. Oligosaccharide analysis by graphitized carbon liquid chromatography-mass spectrometry. Anal. Bioanal. Chem. 2009, 394:163-174.
19. Wuhrer M., Koeleman C.A.M., Deelder A.M., Hokke C.N. Normal-phase nanoscale liquid chromatography-mass spectrometry of underivatized oligosaccharides at low-femtomole sensitivity. Anal. Chem. 2004, 76:833-838.
20. Zhao J., Qiu W.L., Simeone D.M., Lubman D.M. N-linked glycosylation profiling of pancreatic cancer serum using capillary liquid phase separation coupled with mass spectrometric analysis. J. Proteome Res. 2007, 6:1126-1138.
21. Takegawa Y., Deguchi K., Ito H., Keira T., Nakagawa H., Nishimura S.I. Simple separation of isomeric sialylated N-glycopeptides by a zwitterionic type of hydrophilic interaction chromatography. J. Sep. Sci. 2006, 29:2533-2540.
22. Takegawa Y., Deguchi K., Keira T., Ito H., Nakagawa H., Nishimura S. Separation of isomeric 2-aminopyridine derivatized N-glycans and N-glycopeptides of human serum immunoglobulin G by using a zwitterionic type of hydrophilic interaction chromatography. J. Chromatogr. A 2006, 1113:177-181.
23. Morelle W., Michalski J.C. Glycomics and mass spectrometry. Curr. Pharm. Des. 2005, 11:2615-2645.
24. Wagner-Rousset E., Bednarczyk A., Bussat M.C., Colas O., Corvaia N., Schaeffer C., Van Dorsselaer A., Beck A. The way forward, enhanced characterization of therapeutic antibody glycosylation: comparison of three level mass spectrometry-based strategies. J. Chromatogr. B 2008, 872:23-37.
25. Wuhrer M., Deelder A.M. Negative-mode MALDI-TOF/TOF-MS of oligosaccharides labeled with 2-aminobenzamide. Anal. Chem. 2005, 77:6954-6959.
26. Fan J.Q., Kondo A., Kato I., Lee Y.C. High-performance liquid chromatography of glycopeptides and oligosaccharides on graphitized carbon columns. Anal. Biochem. 1994, 219:224-229.