Matrix-assisted laser desorption/ionisation time-offlight (MALDI-TOF) mass spectrometry as a tool for rapid diagnosis of potentially toxigenic Corynebacterium species in the laboratory management of diphtheriaassociated bacteria

Eurosurveillance

Volumn 15, Issue 43, 2010, Pages 1-5

  Konrad, R ^a,b   Berger, A ^a,b   Huber, I ^b   Boschert, V ^b,c   Hormansdorfer S ^b   Busch, U ^b   Hogardt, M ^b,c   Schubert, S ^c   Sing, A ^a,b  

^a National Consiliary Laboratory for Diphtheria   (Germany)

^b Bavarian Health and Food Safety Authority   (Germany)

^c UNIVERSITY OF MUNICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ALGORITHM; ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM ISOLATE; CORYNEBACTERIUM DIPHTHERIAE; CORYNEBACTERIUM PSEUDOTUBERCULOSIS; CORYNEBACTERIUM ULCERANS; DIPHTHERIA; GENE; GENE SEQUENCE; INTERMETHOD COMPARISON; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; RBOB GENE; RELIABILITY; CHEMISTRY; CLASSIFICATION; CORYNEBACTERIUM; GERMANY; HUMAN; ISOLATION AND PURIFICATION; LABORATORY; MASS SPECTROMETRY; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; POLYMERASE CHAIN REACTION;

DIPHTHERIA TOXIN;

ALGORITHMS; BACTERIOLOGICAL TECHNIQUES; CORYNEBACTERIUM; DIPHTHERIA; DIPHTHERIA TOXIN; GERMANY; HUMANS; LABORATORIES; POLYMERASE CHAIN REACTION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 78149371244     PISSN: 1025496X     EISSN: 15607917     Source Type: Journal    
DOI: 10.2807/ese.15.43.19699-en     Document Type: Article

References (19)

1. Neal SE, Efstratiou A. DIPNET - establishment of a dedicated surveillance network for diphtheria in Europe. Euro Surveill. 2007;12(12):pii:754. Available from: http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=754
2. Neal SE, Efstratiou A, DIPNET, International Diphtheria Reference Laboratories. International external quality assurance for the laboratory diagnosis of diphtheria. J Clin Microbiol. 2009;47(12):4037-42.
3. European health for all database (HFA-DB). Copenhagen: World Health Organization Regional Office for Europe. [Accessed 23 July 2010]. Available from: http://data.euro.who.int/hfadb/
4. Diphtheria reported cases. Geneva: World Health Organization. [Accessed 23 July 2010]. Available from: http://www.who.int/immunization_monitoring/en/globalsummary/timeseries/tsincidencedip.htm
5. Hauser D, Popoff MR, Kiredjian M, Boquet P, Bimet F. Polymerase chain reaction assay for diagnosis of potentially toxinogenic Corynebacterium diphtheriae strains: correlation with ADP-ribosylation activity assay. J Clin Microbiol. 1993;31(10):2720-3.
6. Schuhegger R, Lindermayer M, Kugler R, Heesemann J, Busch U, Sing A. Detection of toxigenic Corynebacterium diphtheriae and Corynebacterium ulcerans strains by a novel real-time PCR. J Clin Microbiol. 2008;46(8):2822-3.
7. Engler KH, Glushkevich T, Mazurova IK, George RC, Efstratiou A. A modified Elek test for detection of toxigenic corynebacteria in the diagnostic laboratory. J Clin Microbiol. 1997;35(2):495-8.
8. Khamis A, Raoult D, La Scola B. rpoB gene sequencing for identification of Corynebacterium species. J Clin Microbiol. 2004;42(4):3925-31.
9. Claydon MA, Davey SN, Edwards-Jones V, Gordon DB. The rapid identification of intact microorganisms using mass spectrometry. Nat Biotechnol. 1996;14(11):1584-6.
10. Holland RD, Wilkes JG, Rafii F, Sutherland JB, Persons CC, Voorhees KJ, et al. Rapid identification of intact whole bacteria based on spectral patterns using matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry. Rapid Commun Mass Spectrom. 1996;10(10):1227-32.
11. Seng P, Drancourt M, Gouriet F, La Scola B, Fournier P-E, Rolain JM, et al. Ongoing revolution in bacteriology: Routine identification of bacteria by matrix-asisted laser desorption ionization time-of-flight mass spectrometry. Clin Infect Dis. 2009;49(4):543-51.
12. Van Veen SQ, Claas EC, Kuijper EJ. High-throughput identification of bacteria and yeast by matrix-assisted laser desorption ionization time of flight mass spectrometry in conventional medical microbiology laboratories. J Clin Microbiol. 2010;48(3):900-7.
13. Mellmann A, Cloud J, Maier T, Keckevoet U, Ramminger I, Iwen P, et al. Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to 16S rRNA gene sequencing for species identification of nonfermenting bacteria. J Clin Microbiol. 2008;46(6):1946-54.
14. Sauer S, Kliem M. Mass spectrometry tools for the classification and identification of bacteria. Nat Rev Microbiol. 2010;8(1):74-82.
15. Khamis A, Raoult D, La Scola B. Comparison between rpoB and 16S rRNA gene sequencing for molecular identification of 168 clinical isolates of Corynebacterium. J Clin Microbiol. 2005;43(4):1934-6.
16. Bernard KA, Wiebe D, Burdz T, Reimer A, Ng B, Singh C, et al. Assignment of Brevibacterium stationis (ZoBell and Upham 1944) Breed 1953 to the genus Corynebacterium, as Corynebacterium stationis comb. nov., and emended description of the genus Corynebacterium to include isolates that can alkalinize citrate. Int J Syst Evol Microbiol. 2010;60(Pt4):874-9.
17. Barbuddhe SB, Maier T, Schwarz G, Kostrzewa M, Hof H, Domann E, et al. Rapid identification and typing of listeria species by matrix-assisted laser desorption ionizationtime of flight mass spectrometry. Appl Environ Microbiol. 2008;74(17):5402-7.
18. Wagner KS, Stickings P, White JM, Neal S, Crowcroft NS, Sesardic D, et al. A review of the international issues surrounding the availability and demand for diphtheria antitoxin for therapeutic use. Vaccine. 2009;28(1):14-20.
19. Cianciotto NP, Groman NB. Characterization of bacteriophages from tox-containing, non-toxigenic isolates of Corynebacterium diphtheriae. Microb Pathog. 1997;22(6):343-51.