Detection of toxigenic Corynebacterium diphtheriae and Corynebacterium ulcerans strains by a novel real-time PCR

Journal of Clinical Microbiology

Volumn 46, Issue 8, 2008, Pages 2822-2823

  Schuhegger, Regina ^a   Lindermayer, Marion ^a   Kugler, Rudolf ^a   Heesemann, Jürgen ^b   Busch, Ulrich ^c   Sing, Andreas ^c  

^a National Consiliary Laboratory for Diphtheria   (Germany)

^b UNIVERSITY OF MUNICH   (Germany)

^c BAVARIAN HEALTH AND FOOD SAFETY AUTHORITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DIPHTHERIA TOXIN; PRIMER DNA;

BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM DETECTION; BACTERIUM ISOLATE; CORYNEBACTERIUM DIPHTHERIAE; CORYNEBACTERIUM ULCERANS; DIPHTHERIA; GENE SEQUENCE; HUMAN; LETTER; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; SPECIES DIFFERENTIATION; TOX GENE; ADULT; AGED; BIOSYNTHESIS; CHILD; CORYNEBACTERIUM; FEMALE; GENETICS; GERMANY; ISOLATION AND PURIFICATION; MALE; METHODOLOGY; MIDDLE AGED; POLYMERASE CHAIN REACTION; PRESCHOOL CHILD;

CORYNEBACTERIUM DIPHTHERIAE; CORYNEBACTERIUM ULCERANS;

ADULT; AGED; CHILD; CHILD, PRESCHOOL; CORYNEBACTERIUM; DIPHTHERIA TOXIN; DNA PRIMERS; FEMALE; GERMANY; HUMANS; MALE; MIDDLE AGED; POLYMERASE CHAIN REACTION;

EID: 53149085713     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.01010-08     Document Type: Letter

References (13)

1. Cassiday, P. K., L. C. Pawloski, T. Tiwari, G. N. Sanden, and P. P. Wilkins. 2008. Analysis of toxigenic Corynebacterium ulcerans strains revealing potential for false-negative real-time PCR results. J. Clin. Microbiol. 46:331-333.
2. Hauser, D., M. R. Popoff, M. Kiredjian, P. Boquet, and F. Bimet. 1993. Polymerase chain reaction assay for diagnosis of potentially toxinogenic Corynebacterium diphtheriae strains: correlation with ADP-ribosylation activity assay. J. Clin. Microbiol. 31:2720-2723.
3. Khamis, A., D. Raoult, and B. La Scola. 2004. rpoB gene sequencing for identification of Corynebacterium species. J. Clin. Microbiol. 42:3925-3931.
4. Lartigue, M.-F., X. Monnet, A. Le Flèche, P. A. D. Grimont, J.-J. Benet, A. Durrbach, M. Fabre, and P. Nordmann. 2005. Corynebacterium ulcerans in an immunocompromised patient with diphtheria and her dog. J. Clin. Microbiol. 43:999-1001.
5. Mothershed, E. A., P. K. Cassiday, K. Pierson, L. W. Mayer, and T. Popovic. 2002. Development of a real-time fluorescence PCR assay for rapid detection of the diphtheria toxin gene. J. Clin. Microbiol. 40:4713-4719.
6. Nakao, H., I. K. Mazurova, T. Glushkevich, and T. Popovic. 1997. Analysis of heterogeneity of Corynebacterium diphtheriae toxin gene, tox, and its regulatory element, dtxR, by direct sequencing. Res. Microbiol. 148:45-54.
7. Neal, S., and A. Efstratiou. 2007. DIPNET-establishment of a dedicated surveillance network for diphtheria in Europe. Euro Surveill. 12:E9-E10.
8. Seto, Y., T. Komiya, M. Iwaki, T. Kohda, M. Mukamoto, M. Takahashi, and S. Kozaki. 2008. Properties of corynephage attachment site and molecular epidemiology of Corynebacterium ulcerans isolated from humans and animals in Japan. Jpn. J. Infect. Dis. 61:116-122.
9. Sing, A., S. Bierschenk, and J. Heesemann. 2005. Classical diphtheria caused by Corynebacterium ulcerans in Germany: amino acid sequence differences between diphtheria toxins from Corynebacterium diphtheriae and C. ulcerans. Clin. Infect. Dis. 40:325-326.
10. Sing, A., and J. Heesemann. 2005. Imported cutaneous diphtheria, Germany, 1997-2003. Emerg. Infect. Dis. 11:343-344.
11. Sing, A., M. Hogardt, S. Bierschenk, and J. Heesemann. 2003. Detection of differences in the nucleotide and amino acid sequences of diphtheria toxin from Corynebacterium diphtheriae and Corynebacterium ulcerans causing extrapharyngeal infections. J. Clin. Microbiol. 41:4848-4851.
12. Tiwari, T. S., A. Golaz, D. T. Yu, K. R. Ehresmann, T. F. Jones, H. E. Hill, P. K. Cassiday, L. C. Pawloski, J. S. Moran, T. Popovic, and M. Wharton. 2008. Investigations of 2 cases of diphtheria-like illness due to toxigenic Corynebacterium ulcerans. Clin. Infect. Dis. 46:395-401.
13. Wellinghausen, N., A. Sing, W. V. Kern, S. Perner, R. Marre, and J. Rentschler. 2002. A fatal case of necrotizing sinusitis due to toxigenic Corynebacterium ulcerans. Int. J. Med. Microbiol. 292:59-63.