Two step capture and purification of IgG2 using multicolumn countercurrent solvent gradient purification (MCSGP)

Biotechnology and Bioengineering

Volumn 107, Issue 6, 2010, Pages 974-984

  Muller Spath T ^a,b   Aumann, L ^a,b   Strohlein G ^a,b   Kornmann, H ^c   Valax, P ^c   Delegrange, L ^c   Charbaut, E ^c   Baer, G ^c   Lamproye, A ^c   Johnck M ^d   Schulte, M ^d   Morbidelli, M ^a  

^a ETH ZURICH   (Switzerland)

^b CHROMACON AG   (Switzerland)

^c MERCK SERONO S A   (Switzerland)

^d MERCK KGAA   (Germany)

Author keywords

Continuous chromatography; MCSGP; Monoclonal antibody

Indexed keywords

ANION EXCHANGE; BATCH MODES; BATCH PROCESS; CATION EXCHANGES; CHROMATOGRAPHIC PROCESS; CONTINUOUS CHROMATOGRAPHY; CULTURE SUPERNATANT; GRADIENT ELUTION; MCSGP; MONOCLONAL ANTIBODIES (MAB); MULTI-MODAL; MULTICOLUMN COUNTERCURRENT SOLVENT GRADIENT PURIFICATION; PROTEIN A; STATIONARY PHASE; STATIONARY PHASIS;

BATCH DATA PROCESSING; CELL CULTURE; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; MONOCLONAL ANTIBODIES; POSITIVE IONS; PURIFICATION; SURFACE PLASMON RESONANCE;

BATCH CELL CULTURE;

IMMUNOGLOBULIN G2;

ANION EXCHANGE; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; BATCH PROCESS; CATION EXCHANGE; CELL CULTURE; CHROMATOGRAPHY; ENZYME LINKED IMMUNOSORBENT ASSAY; INTERMETHOD COMPARISON; MULTICOLUMN COUNTERCURRENT SOLVENT GRADIENT PURIFICATION; PRODUCTIVITY; PURIFICATION; QUALITY CONTROL; STEADY STATE;

ANTIBODIES, MONOCLONAL; BIOTECHNOLOGY; CHROMATOGRAPHY, LIQUID; IMMUNOGLOBULIN G; SOLVENTS;

EID: 78149348184     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22887     Document Type: Article

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