Assessment of Clostridium difficile infections by quantitative detection of tcdB toxin by use of a real-time cell analysis system

Journal of Clinical Microbiology

Volumn 48, Issue 11, 2010, Pages 4129-4134

  Ryder, Alex B ^a   Huang, Ying ^b   Li, Haijing ^a   Zheng, Min ^b   Wang, Xiaobo ^b   Stratton, Charles W ^a   Xu, Xiao ^b   Tang, Yi Wei ^a  

^a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b ACEA Biosciences Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

TCDB TOXIN; TOXIN; UNCLASSIFIED DRUG;

ARTICLE; CELL ADHESION; CELL SPREADING; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM DIFFICILE INFECTION; CONCENTRATION (PARAMETERS); ENZYME IMMUNOASSAY; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

BACTERIAL PROTEINS; BACTERIAL TOXINS; BACTERIOLOGICAL TECHNIQUES; CELLS, CULTURED; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM INFECTIONS; FECES; FIBROBLASTS; HUMANS; SENSITIVITY AND SPECIFICITY;

CLOSTRIDIUM DIFFICILE;

EID: 78049517380     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.01104-10     Document Type: Article

References (32)

1. Aslam, S., R. J. Hamill, and D. M. Musher. 2005. Treatment of Clostridium difficile-associated disease: old therapies and new strategies. Lancet Infect. Dis. 5:549-557.
2. Atienza, J. M., J. Zhu, X. Wang, X. Xu, and Y. Abassi. 2005. Dynamic monitoring of cell adhesion and spreading on microelectronic sensor arrays. J. Biomol. Screen. 10:795-805.
3. Bartlett, J. G., and D. N. Gerding. 2008. Clinical recognition and diagnosis of Clostridium difficile infection. Clin. Infect. Dis. 46(Suppl. 1):S12-S18.
4. Chow, W. H., C. McCloskey, Y. Tong, L. Hu, Q. You, C. P. Kelly, H. Kong, Y. W. Tang, and W. Tang. 2008. Application of isothermal helicase-dependent amplification with a disposable detection device in a simple sensitive stool test for toxigenic Clostridium difficile. J. Mol. Diagn. 10:452-458.
5. Cohen, S. H., D. N. Gerding, S. Johnson, C. P. Kelly, V. G. Loo, L. C. McDonald, J. Pepin, and M. H. Wilcox. 2010. Clinical practice guidelines for Clostridium difficile infection in adults: 2010 update by the Society for Healthcare Epidemiology of America (SHEA) and the Infectious Diseases Society of America (IDSA). Infect. Control Hosp. Epidemiol. 31:431-455.
6. Fekety, R. 1997. Guidelines for the diagnosis and management of Clostridium difficile-associated diarrhea and colitis. American College of Gastroenterology, Practice Parameters Committee. Am. J. Gastroenterol. 92:739-750.
7. Gilligan, P. H. 2008. Is a two-step glutamate dehyrogenase[sic] antigen-cytotoxicity neutralization assay algorithm superior to the premier toxin A and B enzyme immunoassay for laboratory detection of Clostridium difficile? J. Clin. Microbiol. 46:1523-1525.
8. He, X., J. Wang, J. Steele, X. Sun, W. Nie, S. Tzipori, and H. Feng. 2009. An ultrasensitive rapid immunocytotoxicity assay for detecting Clostridium difficile toxins. J. Microbiol. Methods 78:97-100.
9. Huang, H., A. Weintraub, H. Fang, and C. E. Nord. 2009. Antimicrobial resistance in Clostridium difficile. Int. J. Antimicrob. Agents 34:516-522.
10. Huang, H., A. Weintraub, H. Fang, and C. E. Nord. 2009. Comparison of a commercial multiplex real-time PCR to the cell cytotoxicity neutralization assay for diagnosis of Clostridium difficile infections. J. Clin. Microbiol. 47:3729-3731.
11. Ke, Y., D. Wu, F. Princen, T. Nguyen, Y. Pang, J. Lesperance, W. J. Muller, R. G. Oshima, and G. S. Feng. 2007. Role of Gab2 in mammary tumorigenesis and metastasis. Oncogene 26:4951-4960.
12. Kelly, C. P., and J. T. LaMont. 2008. Clostridium difficile - more difficult than ever. N. Engl. J. Med. 359:1932-1940.
13. Kvach, E. J., D. Ferguson, P. F. Riska, and M. L. Landry. 2010. Comparison of BD GeneOhm Cdiff real-time PCR assay with a two-step algorithm and a toxin A/B enzyme-linked immunosorbent assay for diagnosis of toxigenic Clostridium difficile infection. J. Clin. Microbiol. 48:109-114.
14. Loo, V. G., L. Poirier, M. A. Miller, M. Oughton, M. D. Libman, S. Michaud, A. M. Bourgault, T. Nguyen, C. Frenette, M. Kelly, A. Vibien, P. Brassard, S. Fenn, K. Dewar, T. J. Hudson, R. Horn, P. Rene, Y. Monczak, and A. Dascal. 2005. A predominantly clonal multi-institutional outbreak of Clostridium difficile-associated diarrhea with high morbidity and mortality. N. Engl. J. Med. 353:2442-2449.
15. Lowy, I., D. C. Molrine, B. A. Leav, B. M. Blair, R. Baxter, D. N. Gerding, G. Nichol, W. D. Thomas, Jr., M. Leney, S. Sloan, C. A. Hay, and D. M. Ambrosino. 2010. Treatment with monoclonal antibodies against Clostridium difficile toxins. N. Engl. J. Med. 362:197-205.
16. McDonald, L. C., G. E. Killgore, A. Thompson, R. C. Owens, Jr., S. V. Kazakova, S. P. Sambol, S. Johnson, and D. N. Gerding. 2005. An epidemic, toxin gene-variant strain of Clostridium difficile. N. Engl. J. Med. 353:2433-2441.
17. Nair, S., D. Yadav, M. Corpuz, and C. S. Pitchumoni. 1998. Clostridium difficile colitis: factors influencing treatment failure and relapse - a prospective evaluation. Am. J. Gastroenterol. 93:1873-1876.
18. Peterson, L. R., R. U. Manson, S. M. Paule, D. M. Hacek, A. Robicsek, R. B. Thomson, Jr., and K. L. Kaul. 2007. Detection of toxigenic Clostridium difficile in stool samples by real-time polymerase chain reaction for the diagnosis of C. difficile-associated diarrhea. Clin. Infect. Dis. 45:1152-1160.
19. Planche, T., A. Aghaizu, R. Holliman, P. Riley, J. Poloniecki, A. Breathnach, and S. Krishna. 2008. Diagnosis of Clostridium difficile infection by toxin detection kits: a systematic review. Lancet Infect. Dis. 8:777-784.
20. Quinn, C. D., S. E. Sefers, W. Babiker, Y. He, R. Alcabasa, C. W. Stratton, K. C. Carroll, and Y. W. Tang. 2010. C. Diff Quik Chek complete enzyme immunoassay provides a reliable first-line method for detection of Clostridium difficile in stool specimens. J. Clin. Microbiol. 48:603-605.
21. Reller, M. E., C. A. Lema, T. M. Perl, M. Cai, T. L. Ross, K. A. Speck, and K. C. Carroll. 2007. Yield of stool culture with isolate toxin testing versus a two-step algorithm including stool toxin testing for detection of toxigenic Clostridium difficile. J. Clin. Microbiol. 45:3601-3605.
22. Reyes, R. C., M. A. John, D. L. Ayotte, A. Covacich, S. Milburn, and Z. Hussain. 2007. Performance of TechLab C. DIFF QUIK CHEK and TechLab C. DIFFICILE TOX A/B II for the detection of Clostridium difficile in stool samples. Diagn. Microbiol. Infect. Dis. 59:33-37.
23. Shin, B. M., E. Y. Kuak, E. J. Lee, and J. G. Songer. 2009. Algorithm combining toxin immunoassay and stool culture for diagnosis of Clostridium difficile infection. J. Clin. Microbiol. 47:2952-2956.
24. Sloan, L. M., B. J. Duresko, D. R. Gustafson, and J. E. Rosenblatt. 2008. Comparison of real-time PCR for detection of the tcdC gene with four toxin immunoassays and culture in diagnosis of Clostridium difficile infection. J. Clin. Microbiol. 46:1996-2001.
25. Solly, K., X. Wang, X. Xu, B. Strulovici, and W. Zheng. 2004. Application of real-time cell electronic sensing (RT-CES) technology to cell-based assays. Assay Drug Dev. Technol. 2:363-372.
26. Stamper, P. D., R. Alcabasa, D. Aird, W. Babiker, J. Wehrlin, I. Ikpeama, and K. C. Carroll. 2009. Comparison of a commercial real-time PCR assay for tcdB detection to a cell culture cytotoxicity assay and toxigenic culture for direct detection of toxin-producing Clostridium difficile in clinical samples. J. Clin. Microbiol. 47:373-378.
27. Suss-Toby, E., J. Zimmerberg, and G. E. Ward. 1996. Toxoplasma invasion: the parasitophorous vacuole is formed from host cell plasma membrane and pinches off via a fission pore. Proc. Natl. Acad. Sci. U. S. A. 93:8413-8418.
28. Swindells, J., N. Brenwald, N. Reading, and B. Oppenheim. 2010. Evaluation of diagnostic tests for Clostridium difficile infection. J. Clin. Microbiol. 48:606-608.
29. Ticehurst, J. R., D. Z. Aird, L. M. Dam, A. P. Borek, J. T. Hargrove, and K. C. Carroll. 2006. Effective detection of toxigenic Clostridium difficile by a two-step algorithm including tests for antigen and cytotoxin. J. Clin. Microbiol. 44:1145-1149.
30. van den Berg, R. J., L. S. Bruijnesteijn van Coppenraet, H. J. Gerritsen, H. P. Endtz, E. R. van der Vorm, and E. J. Kuijper. 2005. Prospective multicenter evaluation of a new immunoassay and real-time PCR for rapid diagnosis of Clostridium difficile-associated diarrhea in hospitalized patients. J. Clin. Microbiol. 43:5338-5340.
31. Xing, J. Z., L. Zhu, J. A. Jackson, S. Gabos, X. J. Sun, X. B. Wang, and X. Xu. 2005. Dynamic monitoring of cytotoxicity on microelectronic sensors. Chem. Res. Toxicol. 18:154-161.
32. Yu, N., J. M. Atienza, J. Bernard, S. Blanc, J. Zhu, X. Wang, X. Xu, and Y. A. Abassi. 2006. Real-time monitoring of morphological changes in living cells by electronic cell sensor arrays: an approach to study G protein-coupled receptors. Anal. Chem. 78:35-43.