Application of isothermal helicase-dependent amplification with a disposable detection device in a simple sensitive stool test for toxigenic Clostridium difficile

Journal of Molecular Diagnostics

Volumn 10, Issue 5, 2008, Pages 452-458

  Chow, Wing Huen A ^a   McCloskey, Cindy ^b   Tong, Yanhong ^a   Hu, Lin ^c   You, Qimin ^c   Kelly, Ciarán P ^d   Kong, Huimin ^a   Tang, Yi Wei ^b   Tang, Wen ^a  

^a BioHelix Corporation   (United States)

^b VANDERBILT UNIVERSITY   (United States)

^c Ustar Biotechnology ^*  (China)

^d BETH ISRAEL DEACONESS MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; GENOMIC DNA; HELICASE;

ARTICLE; BACTERIAL GENE; BACTERIUM IDENTIFICATION; CLOSTRIDIUM DIFFICILE; DISPOSABLE EQUIPMENT; DNA EXTRACTION; FECES ANALYSIS; NONHUMAN; NUCLEIC ACID AMPLIFICATION; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TCDA GENE; TEST STRIP;

CLOSTRIDIUM DIFFICILE;

EID: 52249111798     PISSN: 15251578     EISSN: None     Source Type: Journal    
DOI: 10.2353/jmoldx.2008.080008     Document Type: Article

References (23)

1. Lyerly DM, Krivan HC, Wilkins TD: Clostridium difficile: its disease and toxins. Cün Microbiol Rev 1988, 1:1-18
2. Groschel DH: Clostridium difficile infection- Crit Rev Clin Lab Sci 1996, 3:203-245
3. Banno Y, Kobayashi T, Kono H, Watanabe K, Ueno K, Nozawa Y: Biochemical characterization and biologic actions of two toxins (D-1 and D-2) from Clostridium difficile. Rev Infect Dis 1984, S11-S20
4. Kelly CP, Pothoulakis C, LaMont JT: Clostridium difficile colitis. N Engl J Med 1994, 4:257-262
5. Poutanen SM, Simor AE: Clostridium difficile-associated diarrhea in adults. CMAJ 2004, 1:51-58
6. Kader HA, Piccoli DA, Jawad AF, McGowan KL, Mailer ES: Single toxin detection is inadequate to diagnose Clostridium difficile diarrhea in pediatric patients. Gastroenterology 1998, 6:1329-1334
7. Rupnik M, Braun V, Soehn F, Jane M, Hofstetter M, Laufenberg-Feldmann R, von Eichel-Streiber C: Characterization of polymorphisms In the toxin A and B genes of Clostridium difficile. FEMS Microbiol Lett 1997, 2:197-202
8. Soehn F, Wagenknecht-Wiesner A, Leukel P, Kohl M, Weidmann M, von Eichel-Streiber C, Braun V: Genetic rearrangements In the patho-genicity locus of Clostridium difficile strain 8864: Implications for transcription, expression and enzymatic activity of toxins A and B. Mol Gen Genet 1998, 3:222-232
9. Song KP, Bai XL, Chang SY: Nucleotide and peptide sequences of the open reading frame encoding a truncated toxin A gene of Clostridium difficile strain CCUG 20309. DNA Seq 1999, 2:93-96
10. Sambol SP, Merrigan MM, Lyerly D, Gerding DN, Johnson S: Toxin gene analysis of a variant strain of Clostridium difficile that causes human clinical disease. Infect Immun 2000, 10:5480-5487
11. Alfa MJ, Kabani A, Lyerly D, Moncrief S, Neville LM, Al-Barrak A, Harding GK, Dyck B, Olekson K, Embil JM: Characterization of a toxin A-negative, toxin B-positive strain of Clostridium difficile responsible for a nosocomial outbreak of Clostridium difficile-associated diarrhea. J Clin Microbiol 2000, 7:2706-2714
12. Delmee M: Laboratory diagnosis of Clostridium difficile disease. Clin Microbiol Infect 2001, 8:411-416
13. Peterson LR, Kelly PJ, Nordbrock HA: Role of culture and toxin detection in laboratory testing for diagnosis of Clostridium difficile-associated diarrhea. Eur J Clin Microbiol Infect Dis 1996, 4:330-336
14. Turgeon DK, Novicki TJ, Quick J, Carlson L, Miller P, Ulness B, Cent A, Ashley R, Larson A, Coyle M, Limaye AP, Cookson BT, Fritsche TR: Six rapid tests for direct detection of Clostridium difficile and its toxins in fecal samples compared with the fibroblast cytotoxicity assay. J Clin Microbiol 2003, 2:667-670
15. Belanger SD, Boissinot M, Clairoux N, Picard FJ, Bergeron MG: Rapid detection of Clostridium difficile in feces by real-time PCR. J Clin Microbiol 2003, 2:730-734
16. van den Berg RJ, Kuijper EJ, van Coppenraet LE, Claas EC: Rapid diagnosis of toxinogenic Clostridium difficile in faecal samples with internally controlled real-time PCR. Clin Microbiol Infect 2006, 2:184-186
17. Peterson LR, Manson RU, Paule SM, Hacek DM, Robicsek A, Thomson RB Jr, Kaul KL: Detection of toxigenic Clostridium difficile In stool samples by real-time polymerase chain reaction for the diagnosis of C. difficile-associated diarrhea. Clin Infect Dis 2007, 9:1152-1160
18. An L, Tang W, Ranalli TA, Kim HJ, Wytiaz J, Kong H: Characterization of a thermostable UvrD helicase and Its participation in helicase-dependent amplification. J Biol Chem 2005, 32:28952-28958
19. Goldmeyer J, Kong H, Tang W: Development of a novel one-tube isothermal reverse transcription thermophilic helicase-dependent amplification platform for rapid RNA detection. J Mol Diagn 2007, 5:639-644
20. Trujillo AA, McCaustland KA, Zheng DP, Hadley LA, Vaughn G, Adams SM, Ando T, Glass Rl, Monroe SS: Use of TaqMan real-time reverse transcription-PCR for rapid detection, quantification, and typing of norovirus. J Clin Microbiol 2006, 4:1405-1412
21. Curry SR, Marsh JW, Muto CA, O'Leary MM. Pasculle AW, Harrison LH: tcdC genotypes associated with severe TcdC truncation in an epidemic clone and other strains of Clostridium difficile. J Clin Microbiol 2007, 1:215-221
22. Knoop FC, Owens M, Crocker IC: Clostridium difficile: clinical disease and diagnosis. Clin Microbiol Rev 1993, 3:251-265
23. Jackson CR, Harper JP, Willoughby D, Roden EE, Churchill PF: A simple, efficient method for the separation of humic substances and DNA from environmental samples. Appl Environ Microbiol 1997, 12:4993-4995