Improved safety for molecular diagnosis of classical rabies viruses by use of a TaqMan real-time reverse transcription-PCR "double check" strategy

Journal of Clinical Microbiology

Volumn 48, Issue 11, 2010, Pages 3970-3978

  Hoffmann, B ^a   Freuling, C M ^a   Wakeley, P R ^b   Rasmussen, T B ^c   Leech, S ^b   Fooks, A R ^b   Beer, M ^a   Muller T ^a  

^a FEDERAL RESEARCH INSTITUTE FOR ANIMAL HEALTH   (Germany)

^b VETERINARY LABORATORIES AGENCY   (United Kingdom)

^c TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; ASSAY; DIAGNOSTIC ACCURACY; DIAGNOSTIC PROCEDURE; GENBANK; GENE SEQUENCE; PRIORITY JOURNAL; RABIES VIRUS; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RISK ASSESSMENT; RNA EXTRACTION; SENSITIVITY AND SPECIFICITY; VIRAL GENETICS; VIRUS DETECTION; VIRUS STRAIN;

ANIMALS; DNA PRIMERS; HUMANS; OLIGONUCLEOTIDE PROBES; RABIES; RABIES VIRUS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VIROLOGY;

RABIES VIRUS;

EID: 78049488651     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00612-10     Document Type: Article

References (40)

1. Araújo, D. B., H. Langoni, M. F. Almeida, and J. Megid. 2008. Heminested reverse-transcriptase polymerase chain reaction (hnRT-PCR) as a tool for rabies virus detection in stored and decomposed samples. BMC Res. Notes 1:17.
2. Belák, S., and P. Thoren. 2001. Molecular diagnosis of animal diseases: some experiences over the past decade. Expert. Rev. Mol. Diagn. 1:434-443.
3. Biswal, M., R. Ratho, and B. Mishra. 2007. Usefulness of reverse transcriptase-polymerase chain reaction for detection of rabies RNA in archival samples. Jpn. J. Infect. Dis. 60:298-299.
4. Black, E. M., J. P. Lowings, J. Smith, P. R. Heaton, and L. M. McElhinney. 2002. A rapid RT-PCR method to differentiate six established genotypes of rabies and rabies-related viruses using TaqMan technology. J. Virol. Methods 105:25-35.
5. Black, E. M., L. M. McElhinney, J. P. Lowings, J. Smith, P. Johnstone, and P. R. Heaton. 2000. Molecular methods to distinguish between classical rabies and the rabies-related European bat lyssaviruses. J. Virol. Methods 87:123-131.
6. Crepin, P., L. Audry, Y. Rotivel, A. Gacoin, C. Caroff, and H. Bourhy. 1998. Intravitam diagnosis of human rabies by PCR using saliva and cerebrospinal fluid. J. Clin. Microbiol. 36:1117-1121.
7. David, D., B. Yakobson, D. Rotenberg, N. Dveres, I. Davidson, and Y. Stram. 2002. Rabies virus detection by RT-PCR in decomposed naturally infected brains. Vet. Microbiol. 87:111-118.
8. Dean, D. J., M. K. Abelseth, and P. Athanasiu. 1996. The fluorescence antibody test, p. 88-93. In F.-X. Meslin, M. M. Kaplan, and H. Koprowski (ed.), Laboratory techniques in rabies. World Health Organization, Geneva, Switzerland.
9. Echevarría, J. E., A. Avellon, J. Juste, M. Vera, and C. Ibanez. 2001. Screening of active lyssavirus infection in wild bat populations by viral RNA detection on oropharyngeal swabs. J. Clin. Microbiol. 39:3678-3683.
10. Finnegan, C. J., S. M. Brookes, and A. R. Fooks. 2004. Detection of European bat lyssavirus mRNA in mouse brain by employing in situ hybridisation. J. Virol. Methods 121:221-227.
11. Fooks, A. R., N. Johnson, C. M. Freuling, P. Wakeley, A. Banyard, L. M. McElhinney, D. A. Marston, A. Dastjerdi, E. Wright, R. Weiss, and T. Müller. 2009. Emerging technologies for the detection of rabies virus: challenges and hopes in the 21st century. PLoS Neglect. Trop. D 3:e530.
12. Fooks, A. R., N. Johnson, S. M. Brookes, G. Parsons, and L. M. McElhinney. 2003. Risk factors associated with travel to rabies endemic countries. J. Appl. Microbiol. 94:31-36.
13. Foord, A. J., H. Heine, L. I. Pritchard, R. A. Lunt, K. M. Newberry, C. L. Rootes, and B. D. Boyle. 2006. Molecular diagnosis of lyssaviruses and sequence comparison of Australian bat lyssavirus samples. Aust. Vet. J. 84:225-230.
14. Gibson, U. E., C. A. Heid, and P. M. Williams. 1996. A novel method for real time quantitative RT-PCR. Genome Res. 6:995-1001.
15. Heaton, P. R., P. Johnstone, L. M. McElhinney, R. Cowley, E. O'Sullivan, and J. E. Whitby. 1997. Heminested PCR assay for detection of six genotypes of rabies and rabies-related viruses. J. Clin. Microbiol. 35:2762-2766.
16. Heid, C. A., J. Stevens, K. J. Livak, and P. M. Williams. 1996. Real time quantitative PCR. Genome Res. 6:986-994.
17. Hoffmann, B., T. Harder, E. Starick, K. Depner, O. Werner, and M. Beer. 2007. Rapid and highly sensitive pathotyping of avian influenza A H5N1 virus by using real-time reverse transcription-PCR. J. Clin. Microbiol. 45:600-603.
18. Hoffmann, B., K. Depner, H. Schirrmeier, and M. Beer. 2006. A universal heterologous internal control system for duplex real-time RT-PCR assays used in a detection system for pestiviruses. J. Virol. Methods 136:200-209. (Pubitemid 44051594)
19. Hughes, G. J., I. V. Kuzmin, A. Schmitz, J. Blanton, J. Manangan, S. Murphy, and C. E. Rupprecht. 2006. Experimental infection of big brown bats (Eptesicus fuscus) with Eurasian bat lyssaviruses Aravan, Khujand, and Irkut virus. Arch. Virol. 151:2021-2035.
20. Hughes, G. J., J. S. Smith, C. A. Hanlon, and C. E. Rupprecht. 2004. Evaluation of a TaqMan PCR assay to detect rabies virus RNA: influence of sequence variation and application to quantification of viral loads. J. Clin. Microbiol. 42:299-306.
21. Johnson, N., L. M. McElhinney, J. Smith, P. Lowings, and A. R. Fooks. 2002. Phylogenetic comparison of the genus Lyssavirus using distal coding sequences of the glycoprotein and nucleoprotein genes. Arch. Virol. 147:2111-2123.
22. Kulonen, K., M. Fekadu, S. Whitfield, and C. K. Warner. 1999. An evaluation of immunofluorescence and PCR methods for detection of rabies in archival Carnoy-fixed, paraffin-embedded brain tissue. Zentralbl. Veterinarmed. B 46:151-155.
23. Kuzmin, I. V., G. J. Hughes, A. D. Botvinkin, S. G. Gribencha, and C. E. Rupprecht. 2008. Arctic and Arctic-like rabies viruses: distribution, phylogeny and evolutionary history. Epidemiol. Infect. 136:509-519.
24. Kuzmin, I. V., and C. E. Rupprecht. 2007. Bat rabies, p. 259-307. In A. C. Jackson and W. Wunner. (ed.), Rabies. Academic Press, New York, NY.
25. Nadin-Davis, S. A., M. Sheen, and A. I. Wandeler. 2009. Development of real-time reverse transcriptase polymerase chain reaction methods for human rabies diagnosis. J. Med. Virol. 81:1484-1497.
26. Nagaraj, T., J. P. Vasanth, A. Desai, A. Kamat, S. N. Madhusudana, and V. Ravi. 2006. Ante mortem diagnosis of human rabies using saliva samples: comparison of real time and conventional RT-PCR techniques. J. Clin. Virol. 36:17-23.
27. Orlowska, A., M. Smreczak, P. Trêbas, and J. F. Zmudzinski. 2008. Comparison of realtime PCR and heminested RT-PCR methods in the detection of rabies virus infection in bats and terrestrial animals. Bull. Vet. Inst. Pulawy. 52:313-318.
28. Sacramento, D., H. Bourhy, and N. Tordo. 1991. PCR technique as an alternative method for diagnosis and molecular epidemiology of rabies virus. Mol. Cell Probes 5:229-240.
29. Shankar, V., R. A. Bowen, A. D. Davis, C. E. Rupprecht, and T. J. O'Shea. 2004. Rabies in a captive colony of big brown bats (Eptesicus fuscus). J. Wildl. Dis. 40:403-413.
30. Snow, M., P. McKay, and I. Matejusova. 2009. Development of a widely applicable positive control strategy to support detection of infectious salmon anaemia virus (ISAV) using Taqman real-time PCR. J. Fish Dis. 32:151-156.
31. Tordo, N., A. Benmansour, C. Calisher, R. G. Dietzgen, R. X. Fang, A. O. Jackson, G. Kurath, S. Nadin-Davis, R. B. Tesh, and P. J. Walker. 2004. Rhabdoviridae, p. 623-644. In C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger, and L. A. Ball (ed.), Virus taxonomy, VIIIth report of the ICTV. Elsevier/Academic Press, London, United Kingdom.
32. Tordo, N., D. Sacramento, and H. Bourhy. 1996. The polymerase chain reaction (PCR) technique for diagnosis, typing and epidemiological studies, p. 157-170. In F.-X. Meslin, M. M. Kaplan, and H. Koprowski (ed.), Laboratory techniques in rabies, 4th ed. World Health Organization, Geneva, Switzerland.
33. Tordo, N., H. Bourhy, and D. Sacramento. 1995. PCR technology for lyssavirus diagnosis, p. 125-145. In J. P. Clewley (ed.), Polymerase chain reaction (PCR) for human viral diagnosis. CRC, Boca Raton, FL.
34. Toussaint, J. F., C. Sailleau, E. Breard, S. Zientara, and K. De Clercq. 2007. Bluetongue virus detection by two real-time RT-qPCRs targeting two different genomic segments. J. Virol. Methods 140:115-123.
35. Vázquez-Morón, S., A. Avellon, and J. E. Echevarria. 2006. RT-PCR for detection of all seven genotypes of Lyssavirus genus. J. Virol. Methods 135:281-287.
36. Wacharapluesadee, S., J. Sutipanya, S. Damrongwatanapokin, P. Phumesin, P. Chamnanpood, C. Leowijuk, and T. Hemachudha. 2008. Development of a TaqMan real-time RT-PCR assay for the detection of rabies virus. J. Virol. Methods 151:317-320.
37. Wakeley, P. R., N. Johnson, L. M. McElhinney, D. Marston, J. Sawyer, and A. R. Fooks. 2005. Development of a real-time, TaqMan reverse transcription-PCR assay for detection and differentiation of lyssavirus genotypes 1, 5, and 6. J. Clin. Microbiol. 43:2786-2792.
38. Webster, W. A., and G. A. Casey. 1996. Virus isolation in neuroblastoma cell culture, p. 93-104. In F.-X. Meslin, M. M. Kaplan, and H. Koprowski (ed.), Laboratory techniques in rabies. World Health Organization, Geneva, Switzerland.
39. Whitby, J. E., P. R. Heaton, E. M. Black, M. Wooldridge, L. M. McElhinney, and P. Johnstone. 2000. First isolation of a rabies-related virus from a Daubenton's bat in the United Kingdom. Vet. Rec. 147:385-388.
40. World Health Organisation. 2005. Expert consultation on rabies, first report. World Health Organ. Tech. Rep. Ser. 931:1-121.