Development of a real-time, TaqMan reverse transcription-PCR assay for detection and differentiation of lyssavirus genotypes 1, 5, and 6

Journal of Clinical Microbiology

Volumn 43, Issue 6, 2005, Pages 2786-2792

  Wakeley, P R ^a,b   Johnson, N ^a   McElhinney, L M ^a   Marston, D ^a   Sawyer, J ^a   Fooks, A R ^a  

^a WORLD HEALTH ORGANIZATION   (Switzerland)

^b VETERINARY LABORATORIES AGENCY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; GENE SEQUENCE; GENETIC VARIABILITY; GENOTYPE; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RABIES VIRUS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRUS DETECTION; VIRUS GENE; VIRUS ISOLATION;

ANIMALS; CATS; CATTLE; CELL LINE; CHIROPTERA; DOGS; EUROPE; GENOTYPE; HUMANS; LYSSAVIRUS; MICE; RABIES; RABIES VIRUS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RHABDOVIRIDAE INFECTIONS; RNA, VIRAL; TAQ POLYMERASE;

LYSSAVIRUS; RABIES VIRUS;

EID: 20444455859     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.43.6.2786-2792.2005     Document Type: Article

References (31)

1. Arai, Y. T., I. V. Kuzmin, Y. Kameoka, and A. D. Botvinkin. 2003. New lyssavirus genotype from the lesser mouse-eared bat (Myotis blythi), Kyrghyzstan. Emerg. Infect. Dis. 9:333-337.
2. Badrane, H., C. Bahloul, P. Perrin, and N. Tordo. 2001. Evidence of two lyssavirus phylogroups with distinct pathogenicity and immunogenicity. J. Virol. 75:3268-3276.
3. Black, E. M., J. P. Lowings, J. Smith, P. R. Heaton, and L. M. MeElhinney. 2002. A rapid RT-PCR method to differentiate six established genotypes of rabies and rabies-related viruses using TaqMan technology. J. Virol. Methods 105:25-35.
4. Bourhy, H., B. Kissi, M. Lafon, D. Sacramento, and N. Tordo. 1992. Antigenic and molecular characterization of bat rabies in Europe. J. Clin. Microbiol. 30:2419-2426.
5. Bourhy, H., B. Kissi, and N. Tordo. 1993. Molecular diversity of the Lyssa-virus genus. Virology 194:70-81.
6. Dean, D. J., M. K. Abelseth, and P. Atanasiu. 1996. The fluorescence antibody test, p. 88-93. In F. X. Meslin, M. M. Kaplan, and H. Koprowski (ed.), Laboratory techniques in rabies, 4th ed. World Health Organization, Geneva, Switzerland.
7. Department of Health. 2000. Policy and guidance article. Memorandum on rabies: prevention and control, p. 19. Department of Health, London, United Kingdom.
8. Fooks, A. R., L. M. MeElhinney, D. A. Marston, D. Selden, T. A. Joliffe, P. R. Wakeley, N. Johnson, and S. M. Brookes. 2004. Identification of a European bat lyssavirus type 2 in a Daubenton's bat found in Staines, Surrey, UK. Vet. Rec. 155:434-435.
9. Fooks, A. R., L. M. MeElhinney, D. J. Pounder, C. J. Finnegan, K. Mansfield, N. Johnson, S. M. Brookes, G. Parsons, K. White, P. G. McIntyre, and D. Nathwani. 2003. Case report: isolation of a European bat lyssavirus type 2a from a fatal human case of rabies encephalitis. J. Med. Virol. 71:281-289.
10. Fooks, A. R., D. Seiden, S. M. Brookes, N. Johnson, D. A. Marston, T. A. Joliffe, P. R. Wakeley, and L. M. McElhinney. 2004. Identification of a European bat lyssavirus type 2 in a Daubenton's bat found in Lancashire. Vet. Rec. 155:606-607.
11. Gould, A. R., A. D. Hyatt, R. Lunt, J. A. Kattenbelt, S. Hengstberger, and S. Blacksell. 1998. Characterisation of a novel lyssavirus isolated from Pteropid bats in Australia. Virus Res. 54:165-187.
12. Heaton, P. R., P. Johnstone, L. M. McElhinney, R. Cowley, E. O'Sullivan, and J. E. Whitby. 1997. Heminested PCR assay for the detection of six genotypes of rabies and rabies-related viruses. J. Clin. Microbiol. 35:2762-2766.
13. Heaton, P. R., L. M. McElhinney, and J. P. Lowings. 1999. Detection and identification of rabies and rabies-related viruses using rapid-cycle PCR. J. Virol. Methods 81:63-69.
14. Hughes, G. J., J. S. Smith, C. A. Hanlon, and C. E. Rupprecht. 2004. Evaluation of a TaqMan PCR assay to detect rabies virus RNA: influence of sequence variation and application to quantification of viral loads. J. Clin. Microbiol. 42:299-306.
15. Johnson, N., D. W. Lipscomb, R. Stott, G. Gopal Rao, K. Mansfield, J. Smith, L. M. McElhinney, and A. R. Fooks. 2002. Investigation of a human case of rabies in the United Kingdom. J. Clin. Virol. 25:351-356.
16. Johnson, N., L. M. McElhinney, Y. H. Ali, I. K. Saeed, and A. R. Fooks. 2004. Molecular epidemiology of canid rabies in Sudan: evidence for a common origin of rabies with Ethiopia. Virus Res. 104:201-205.
17. Johnson, N., D. Selden, G. Parsons, D. Healy, S. M. Brookes, L. M. Mc-Elhinney, A. M. Hutson. and A. R. Fooks. 2003. Isolation of a European bat lyssavirus type 2 from a Daubenton's bat in the United Kingdom. Vet. Rec. 152:383-387.
18. King, A., and J. Crick. 1988. Rabies-related viruses, p. 177-199. In J. B. Campbell and K. M. Charlton (ed.), Rabies. Kluwer, Boston, Mass.
19. Koprowski, H. 1996. The mouse inoculation test, p. 80-87. In F. X. Meslin, M. M. Kaplan, and H. Koprowski (ed.), Laboratory techniques in rabies, 4th ed. World Health Organization, Geneva, Switzerland.
20. Livak, K. J., S. J. A. Flood, J. Marmaro, W. Giusti, and K. Deetz. 1995. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridisation. PCR Methods Appl. 4:357-362.
21. Nadin-Davis, S. A., M. Abdel-Malik, J. Armstrong, and A. I. Wandeler. 2002. Lyssavirus P gene characterisation provides insight into the phylogeny of the genus and identifies structural similarities and diversity within the encoded phosphoprotein. Virology 298:286-305.
22. Schneider, L. G., and J. H. Cox. 1994. Bat lyssaviruses in Europe, p. 207-218. In C. E. Rupprecht, B. Dietzschold, and H. Koprowski (ed.), Lyssaviruses. Springer-Verlag, Berlin, Germany.
23. Smith, I. L., J. A. Northill, B. J. Harrower, and G. A. Smith. 2002. Detection of Australian bat lyssavirus using a fluorogenic probe. J. Clin. Virol. 25:285-291.
24. Smith, J., L. McElhinney, G. Parsons, N. Brink, T. Doherty, D. Agranoff, M. E. Miranda, and A. R. Fooks. 2003. Case report: rapid ante-mortem diagnosis of a human case of rabies imported into the UK from the Philippines. J. Med. Virol. 69:150-155.
25. Smith, S., L. Vigilant, and P. A. Morin. 2002. The effect of sequence length and oligonucleotide mismatches on 5′ exonuclease assay efficiency. Nucleic Acids Res. 30:e111.
26. Tordo, N., O. Poch, A. Ermine, G. Keith, and F. Rougeon. 1988. Completion of the rabies virus genome sequence determination: highly conserved domains among the L (polymerase) proteins of unsegmented negative-strand RNA viruses. Virology 165:565-576.
27. Vandesomple, J., A. De Paepe, and F. Speleman. 2002. Elimination of primer dimer artefacts and genomic co-amplification using a two-step SYBR green I real-time RT-PCR. Anal. Biochem. 303:85-98.
28. Webster, W. A., and G. A. Casey. 1996. Virus isolation in neuroblastoma cell culture, p. 96-104. In F. X. Meslin, M. M. Kaplan, and H. Koprowski (ed.), Laboratory techniques in rabies, 4th ed. World Health Organization, Geneva, Switzerland.
29. Whitby, J. E., P. Johnstone, and C. Sillero-Zubiri. 1997. Rabies virus in the decomposed brain of an Ethiopian wolf detected by nested reverse transcription-polymerase chain reaction. J. Wild1. Dis. 33:912-915.
30. Whitby, J. E., P. R. Heaton, E. M. Black, M. Wooldridge, L. M. McElhinney, and P. Johnstone. 2000. First isolation of a rabies-related virus from a Daubenton's bat in the United Kingdom. Vet. Rec. 147:385-388.
31. World Health Organization. 2002. World survey for rabies no. 35 for the year 1999. [Online.] http://www.who.int/emc-documents/rabies/whocdscreph200210. html.