Expression and purification of 15 kDa granulysin utilizing an insect cell secretion system

Protein Expression and Purification

Volumn 75, Issue 1, 2011, Pages 70-74

  Finn, Michael W ^a   Clayberger, Carol ^a   Krensky, Alan M ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Granulysin; Insect cell expression; Monocytes; NK cells; Recombinant protein; T cells

Indexed keywords

GNLY PROTEIN, HUMAN; ISOPROTEIN; T LYMPHOCYTE ANTIGEN;

ANIMAL; ARTICLE; BACULOVIRUS; CELL LINE; CYTOLOGY; GENE EXPRESSION; GENE VECTOR; GENETICS; HUMAN; INSECT; ISOLATION AND PURIFICATION;

ANIMALS; ANTIGENS, DIFFERENTIATION, T-LYMPHOCYTE; BACULOVIRIDAE; CELL LINE; GENE EXPRESSION; GENETIC VECTORS; HUMANS; INSECTS; PROTEIN ISOFORMS;

HEXAPODA;

EID: 78049302506     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.07.009     Document Type: Article

References (19)

1. A.M. Krensky, and C. Clayberger Granulysin: a novel host defense molecule Am. J. Transplant. 5 2005 1789 1792
2. J. Kumar, S. Okada, C. Clayberger, and A.M. Krensky Granulysin: a novel antimicrobial Expert Opin. Investig. Drugs 10 2001 321 329
3. D.A. Hanson, A.A. Kaspar, F.R. Poulain, and A.M. Krensky Biosynthesis of granulysin, a novel cytolytic molecule Mol. Immunol. 36 1999 413 422
4. W.C. Manning, S. O'Farrell, T.J. Goralski, and A.M. Krensky Genomic structure and alternative splicing of 519, a gene expressed late after T cell activation J. Immunol. 148 1992 4036 4042
5. S.V. Pena, D.A. Hanson, B.A. Carr, T.J. Goralski, and A.M. Krensky Processing, subcellular localization, and function of 519 (granulysin), a human late T cell activation molecule with homology to small, lytic, granule proteins J. Immunol. 158 1997 2680 2688
6. W.A. Ernst, S. Thoma-Uszynski, R. Teitelbaum, C. Ko, D.A. Hanson, C. Clayberger, A.M. Krensky, M. Leippe, B.R. Bloom, T. Ganz, and R.L. Modlin Granulysin, a T cell product, kills bacteria by altering membrane permeability J. Immunol. 165 2000 7102 7108
7. S. Gamen, D.A. Hanson, A. Kaspar, J. Naval, A.M. Krensky, and A. Anel Granulysin-induced apoptosis. I. Involvement of at least two distinct pathways J. Immunol. 161 1998 1758 1764
8. S. Stenger, D.A. Hanson, R. Teitelbaum, P. Dewan, K.R. Niazi, C.J. Froelich, T. Ganz, S. Thoma-Uszynski, A. Melian, C. Bogdan, S.A. Porcelli, B.R. Bloom, A.M. Krensky, and R.L. Modlin An antimicrobial activity of cytolytic T cells mediated by granulysin Science 282 1998 121 125 (Pubitemid 28471143)
9. T. Yabe, C. McSherry, F.H. Bach, and J.P. Houchins A cDNA clone expressed in natural killer and T cells that likely encodes a secreted protein J. Exp. Med. 172 1990 1159 1163
10. A. Deng, S. Chen, Q. Li, S.C. Lyu, C. Clayberger, and A.M. Krensky Granulysin, a cytolytic molecule, is also a chemoattractant and proinflammatory activator J. Immunol. 174 2005 5243 5248
11. R. Hopkins, and D. Esposito A rapid method for titrating baculovirus stocks using the Sf-9 easy titer cell line Biotechniques 47 2009 785 788
12. C. Clayberger, and A.M. Krensky Granulysin Curr. Opin. Immunol. 15 2003 560 565
13. T.J. Schall, J. Jongstra, B.J. Dyer, J. Jorgensen, C. Clayberger, M.M. Davis, and A.M. Krensky A human T cell-specific molecule is a member of a new gene family J. Immunol. 141 1988 1018 1025
14. P.L. Wang, S. O'Farrell, C. Clayberger, and A.M. Krensky Identification and molecular cloning of tactile. A novel human T cell activation antigen that is a member of the Ig gene superfamily J. Immunol. 148 1992 2600 2608
15. W.H. Chung, S.I. Hung, J.Y. Yang, S.C. Su, S.P. Huang, C.Y. Wei, S.W. Chin, C.C. Chiou, S.C. Chu, H.C. Ho, C.H. Yang, C.F. Lu, J.Y. Wu, Y.D. Liao, and Y.T. Chen Granulysin is a key mediator for disseminated keratinocyte death in Stevens-Johnson syndrome and toxic epidermal necrolysis Nat. Med. 14 2008 1343 1350
16. M.J. Koh, and Y.K. Tay An update on Stevens-Johnson syndrome and toxic epidermal necrolysis in children Curr. Opin. Pediatr. 21 2009 505 510
17. X. Chen, C.H. Chang, and D.M. Goldenberg Novel strategies for improved cancer vaccines Expert Rev. Vaccines 8 2009 567 576
18. T.H. Han, P. Jin, J. Ren, S. Slezak, F.M. Marincola, and D.F. Stroncek Evaluation of 3 clinical dendritic cell maturation protocols containing lipopolysaccharide and interferon-gamma J. Immunother. 32 2009 399 407
19. R.M. Steinman, and J. Banchereau Taking dendritic cells into medicine Nature 449 2007 419 426