A novel micropit device integrates automated cell positioning by dielectrophoresis and nuclear transfer by electrofusion

Biomedical Microdevices

Volumn 12, Issue 5, 2010, Pages 777-786

  Clow, Andrew L ^a   Gaynor, Paul T ^a   Oback, Björn J ^b  

^a UNIVERSITY OF CANTERBURY   (New Zealand)

^b RUAKURA RESEARCH CENTRE   (New Zealand)

Author keywords

Cloning; Dielectrophoresis; Electrofusion; Nuclear transfer

Indexed keywords

AUTOMATED CELL; BOROSILICATE GLASS SUBSTRATES; CELL POSITIONING; CIRCULAR HOLES; DIELECTROPHORESIS; DONOR CELLS; ELECTROFUSION; FILM ELECTRODES; INSULATING FILM; ISOLATED CELLS; MICRO-FLUIDIC DEVICES; MICROPITS; NUCLEAR TRANSFER; PARALLEL PLATES; PHOTOSENSITIVE EPOXY; PROOF OF PRINCIPLES; SOMATIC CELLS; TITANIUM ELECTRODES; TWO LAYERS;

AUTOMATION; BOROSILICATE GLASS; CELL CULTURE; CLONING; ELECTRODES; ELECTROPHORESIS; FLUIDIC DEVICES; SUBSTRATES; TITANIUM;

CLONE CELLS;

BUFFER; GLASS; POLYMER; TITANIUM;

ANIMAL CELL; ARTICLE; AUTOMATION; CELL FUNCTION; CELL ISOLATION; CELL NUCLEUS TRANSPLANTATION; CONTROLLED STUDY; ELECTROFUSION (CELL); ELECTROPHORESIS; FIBROBLAST; MATERIAL COATING; MICROELECTRODE; MICROFILM; MICROFLUIDICS; MICROTECHNOLOGY; NONHUMAN; OOCYTE; OSMOLARITY; OVARY FOLLICLE CELL; PHOTOSENSITIZATION; PRIORITY JOURNAL; SOMATIC CELL; STEREOMICROSCOPY; THICKNESS;

ANIMALS; AUTOMATION; CATTLE; CELL FUSION; ELECTRODES; ELECTROPHORESIS; FEMALE; MICROFLUIDIC ANALYTICAL TECHNIQUES; MODELS, THEORETICAL; NUCLEAR TRANSFER TECHNIQUES; OOCYSTS; SYSTEMS INTEGRATION;

BOVINAE;

EID: 78049283274     PISSN: 13872176     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10544-010-9432-3     Document Type: Article

References (34)

1. M. M. Alkaisi, J. J. Muys, J. J. Evans, Bioimprint replication of single cells on a biochip, in BioMEMS and Nanotechnol. III, U212 (SPIE, 2007)
2. Y. H. Anis, M. R. Holl, D. R. Meldrum, Automated vision-based selection and placement of single cells in microwell array formats, in Autom. Sci. and Eng., 2008. CASE 2008. IEEE Int. Conf. on, 315 (2008)
3. W.M. Arnold, N.R. Franich, Cell isolation and growth in electric-field defined micro-wells. Curr. Appl. Phys. 6, 371 (2006)
4. W. Choi, J.S. Kim, D.H. Lee, K.K. Lee, D.B. Koo, J.K. Park, Dielectrophoretic oocyte selection chip for in vitro fertilization. Biomed. Microdevices 10, 337 (2008)
5. M.R. Davey, P. Anthony, J.B. Power, K.C. Lowe, Plant protoplasts: status and biotechnological perspectives. Biotechnol. Adv. 23, 131 (2005)
6. Y. Du, P.M. Kragh, X. Zhang, S. Purup, H. Yang, L. Bolund, G. Vajta, High overall in vitro efficiency of porcine handmade cloning (HMC) combining partial zona digestion and oocyte trisection with sequential culture. Cloning and Stem Cells 7, 199 (2005)
7. P. Gaynor, D. Wells, B. Oback, Couplet alignment and improved electrofusion by dielectrophoresis for a zona-free highthroughput cloned embryo production system. Med. Biol. Eng. Comput. 43, 150 (2005)
8. D.S. Gray, J.L. Tan, J. Voldman, C.S. Chen, Dielectrophoretic registration of living cells to a microelectrode array. Biosens. Bioelectron. 19, 1765 (2004)
9. T. Hayashi, H. Tanaka, J. Tanaka, R. Wang, B.J. Averbook, P.A. Cohen, S. Shu, Immunogenicity and therapeutic efficacy of dendritic-tumor hybrid cells generated by electrofusion. Clin. Immunol. 104, 14 (2002)
10. W. Hayt, Engineering Electromagnetics (McGraw-Hill, New York, 1967)
11. T.B. Jones, Electromechanics of Particles (Cambridge University Press, Cambridge, 1995)
12. R.S. Kane, S. Takayama, E. Ostuni, D.E. Ingber, G.M. Whitesides, Patterning proteins and cells using soft lithography. Biomater. 20, 2363 (1999)
13. Y. Kimura, M. Gel, B. Techaumnut, K. Tsuda, H. Oana, H. Kotera, T. Tada, M.Washizu, High-yield parallel electro-fusion device based on field constriction at an orifice array. Proc. 12th Int. Conf. on Miniat. Syst. for Chem. and Life Sci. (MicroTAS), 540 (2008)
14. G. Kohler, C. Milstein, Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256, 495 (1975)
15. M. Lian, N. Islam, J. Wu, AC electrothermal manipulation of conductive fluids and particles for lab-chip applications. Nanobiotechnol., IET 1, 36 (2007)
16. S. Masuda, M. Washizu, T. Nanba, Novel method of cell fusion in field constriction area in fluid integrated circuit. IEEE Trans. Ind. Appl. 25, 732 (1989)
17. J. Muys, M.M. Alkaisi, J.J. Evans, J. Nagase, Biochip: cellular analysis by atomic force microscopy using dielectrophoretic manipulation. Jpn. J. Appl. Phys. Part 1 - Regul. Pap. Brief Commun. Rev. Pap. 44, 5717 (2005)
18. K. Nolkrantz, C. Farre, A. Brederlau, R.I.D. Karlsson, C. Brennan, P. S. Eriksson, S.G. Weber, M. Sandberg, O. Orwar, Electroporation of single cells and tissues with an electrolyte-filled capillary. Anal. Chem. 73, 4469 (2001)
19. B. Oback, D.N. Wells, Cloning cattle. Cloning and Stem Cells 5, 243 (2003)
20. B. Oback, A.T. Wiersema, P. Gaynor, G. Laible, F.C. Tucker, J.E. Oliver, A.L. Miller, H.E. Troskie, K.L. Wilson, J.T. Forsyth, M. C. Berg, K. Cockrem, V. McMillan, H.R. Tervit, D.N. Wells, Cloned cattle derived from a novel zona-free embryo reconstruction system. Cloning and Stem Cells 5, 3 (2003)
21. H.A. Pohl, Dielectrophoresis (Cambridge University Press, Cambridge, 1978)
22. J. Schaper, H. Bohnenkamp, T. Noll, in Cell Technol. for Cell Prod., (2007), p.207
23. A.M. Skelley, O. Kirak, H. Suh, R. Jaenisch, J. Voldman, Microfluidic control of cell pairing and fusion. Nat. Meth. 6, 147 (2009)
24. T. Suzuki, Y. Hirabayashi, I. Kanno, M. Washizu, H. Kotera, Assembly-free microfabrication process for multi-layered microfluidic networks using single-mask multidirectional photolithography. Proc. 12th Int. Conf. on Miniat. Syst. for Chem. and Life Sci. (MicroTAS), 363 (2008).
25. M. Tada, T. Tada, L. Lefebvre, S.C. Barton, M.A. Surani, Embryonic germ cells induce epigenetic reprogramming of somatic nucleus in hybrid cells. Embo J. 16, 6510 (1997)
26. M. Tada, Y. Takahama, K. Abe, N. Nakatsuji, T. Tada, Nuclear reprogramming of somatic cells by in vitro hybridization with ES cells. Curr. Biol. 11, 1553 (2001)
27. B. Techaumnat, M. Washizu, Analysis of the effects of an orifice plate on the membrane potential in electroporation and electrofusion of cells. J. Phys. D. Appl. Phys. 40, 1831 (2007)
28. B. Techaumnat, K. Tsuda, O. Kurosawa, G. Murat, M. Washizu, Highyield electrofusion of cells using electric-field constriction. 2007 Int. Symp. onMicro-NanoMechatron. and Hum. Sci. 1 and 2, 38 (2007)
29. Y.-S. Torisawa, B. Mosadegh, G. D. Luker, S. Takayama, Hydrodynamic cellular patterning for 3D Co-culture. Proc. 12th Int. Conf. on Miniat. Syst. for Chem. and Life Sci. (MicroTAS), 27 (2008)
30. Z. Ulanowski, I.K. Ludlow, Compact optical trapping microscope using a diode laser. Meas. Sci. Technol. 11, 1778 (2000)
31. M. Washizu, T. Nanba, S. Masuda, Handling biological cells using a fluid integrated circuit. IEEE Trans. Ind. Appl. 26, 352 (1990)
32. D.N. Wells, P.M. Misica, H.R. Tervit, Production of cloned calves following nuclear transfer with cultured adult mural granulosa cells. Biol. Reprod. 60, 996 (1999)
33. I. Wilmut, A.E. Schnieke, J. McWhir, A.J. Kind, K.H.S. Campbell, Viable offspring derived from fetal and adult mammalian cells. Nature 385, 810 (1997)
34. U. Zimmermann, G.A. Neil, Electromanipulation of Cells (CRC Press, Boca Raton, 1996)