Couplet alignment and improved electrofusion by dielectrophoresis for a zona-free high-throughput cloned embryo production system

Medical and Biological Engineering and Computing

Volumn 43, Issue 1, 2005, Pages 150-154

  Gaynor, Paul ^a   Wells, D N ^b   Oback, B ^b  

^a UNIVERSITY OF WAIKATO   (New Zealand)

^b RUAKURA RESEARCH CENTRE   (New Zealand)

Author keywords

Cloning; Dielectrophoresis; Electrofusion; Nuclear transfer; Zona free

Indexed keywords

AGRICULTURE; CELLS; DRUG PRODUCTS; ELECTRIC FIELDS; ELECTRODES; ELECTROPHORESIS; TISSUE; TRANSPLANTATION (SURGICAL);

BIOPHARMACEUTICALS; ELECTROFUSION; LIVESTOCK BREEDING; SOMATIC NUCLEAR TRANSFER;

CLONING;

AGRICULTURE; ALTERNATING CURRENT; ANIMAL CELL; ANIMAL GENETICS; ARTICLE; AUTOMATION; BIOMEDICINE; BREEDING METHOD; CELL NUCLEUS TRANSPLANTATION; CLONING; CONTROLLED STUDY; CYTOPLASM; DIRECT CURRENT; ELECTRIC FIELD; ELECTRODE; ELECTROFUSION (CELL); ELECTROPHORESIS; FETUS; LIVESTOCK; MAMMAL CELL; NONHUMAN; SOMATIC CELL; TISSUE TRANSPLANTATION;

ANIMALS; CATTLE; CLONING, ORGANISM; ELECTRIC STIMULATION; ELECTROPHORESIS; FEMALE; MALE; NUCLEAR TRANSFER TECHNIQUES; ZONA PELLUCIDA;

AGRICULTURE; CELLS; CLONES; DRUGS; ELECTRIC FIELDS; ELECTRODES; ELECTROPHORESIS;

EID: 14044276358     PISSN: 01400118     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02345137     Document Type: Article

References (16)

1. Campbell, K. H., Loi, P., Cappai, P., and Wilmut, I. (1994): 'Improved development to blastocyst of ovine nuclear transfer embryos reconstructed during the presumptive S-phase of enucleated activated oocytes', Biol. Reprod., 50, pp. 1385-1393
2. Campbell, K. H., McWhir, J., Ritchie, W. A., and Wilmut, I. (1996): 'Sheep cloned by nuclear transfer from a cultured cell line', Nature, 380, pp. 64-66
3. Colman, A., and Kind, A. (2000): 'Therapeutic cloning: concepts and practicalities', Trends Biotechnol., 18, pp. 192-196
4. Jones, T. (1995): 'Electromechanics of particles' (Cambridge University Press, USA, 1995)
5. Kuroiwa, Y., Kasinathan, P., Choi, Y. J., Naeem, R., Tomizuka, K., Sullivan, E. J., Knott, J. G., Duteau, A., Goldsby, R. A., Osborne, B. A., Ishida, I., and Robl, J. M. (2002): 'Cloned transchromosomic calves producing human immunoglobulin', Nat. Biotechnol., 20, pp. 889-894
6. Lagutina, I., Crotti, G., Colleoni, S., Ponderato, N., Duchi, R., Lazzari, G., and Galli, C. (2003): 'Nuclear transfer in horses', Theriogenology, 59, p. 269
7. Lanza, R. P., Chung, H. Y., Yoo, J. J., Wettstein, P. J., Blackwell, C., Borson, N., Hofmeister, E., Schuch, G., Soker, S., Moraes, C. T., West, M. D., and Atala, A. (2002): 'Generation of histocompatible tissues using nuclear transplantation', Nat. Biotechnol., 20, pp. 689-696
8. Neil, G., and Zimmermann, U. (1993): 'Electrofusion', Methods Enzymol., 220, pp. 174-196
9. Neumann, E., Sowers, A., and Jordan, C. (Eds) (1989): 'Electroporation and electrofusion in cell biology' (Plennum Press, New York, 1989)
10. Oback, B., Wiersma, A., Gaynor, P., Laible, G., Tucker, F., Oliver, J., Miller, A., Troskie, H., Wilson, K., Forsyth, J., Berg, M., Cockrem, K., McMillan, V., Tervit, H., and Wells, D. (2003): 'Cloned cattle derived from a novel zona-free in vitro production system', Cloning Stem Cells, 5, pp. 3-12
11. Oback, B., and Wells, D. N. (2003): 'Cloning cattle', Cloning Stem Cells, 5, pp. 243-256
12. Robertson, I., and Nelson, R. (1998): 'Certification and identification of the embryo', in Stringfellow, D. A., and Seidel, S. M., (Eds): 'Manual of the International Embryo Transfer Society' (International Embryo Transfer Society, Illinois, 1998), pp. 103-134
13. Solter, D. (2000): 'Mammalian cloning: advances and limitations', Nat. Rev. Genet., 1, pp. 199-207
14. Thompson, J. G., McNaughton, C., Gasparrini, B., McGowan, L.T., and Tervit, H. R. (2000): 'Effect of inhibitors and uncouplers of oxidative phosphorylation during compaction and blastulation of bovine embryos cultured in vitro', J. Reprod. Fertil., 118, pp. 47-55
15. Wells, D., Misica, P., and Tervit, H. (1999): 'Production of cloned calves following nuclear transfer with cultured adult mural granulosa cells', Biol. Reprod., 60, pp. 996-1005
16. Wells, D. N. (2003): 'Cloning in livestock agriculture', Reproduction, Suppl. 61, pp. 131-150