Purification and characterization of a cysteine endopeptidase from vasconcellea quercifolia A. St.-Hil. latex displaying high substrate specificity

Journal of Agricultural and Food Chemistry

Volumn 58, Issue 20, 2010, Pages 11027-11035

  Torres, M José ^a   Trejo, Sebastián A ^b   Martin, M Inés ^a   Natalucci, Claudia L ^a   AviléS, Francesc X ^b   López, Laura M I ^a  

^a UNIVERSIDAD NACIONAL DE LA PLATA   (Argentina)

^b UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

Author keywords

Caricaceae; Latex; Papain; Plant cysteine protease; Vasconcellea quercifolia

Indexed keywords

CARICA PAPAYA; CARICACEAE; CASEIN HYDROLYSATE; CYSTEINE PROTEASE; ENZYMATIC PREPARATION; ION EXCHANGE CHROMATOGRAPHY; ISO-ELECTRIC POINTS; MALDI-TOF; N-TERMINALS; PAPAIN; PLANT CYSTEINE PROTEASE; PROTEOLYTIC ACTIVITIES; SDS-PAGE; SEARCH TOOLS; SPECIFIC ACTIVITY; SUBSTRATE SPECIFICITY; TRYPTIC PEPTIDES; VASCONCELLEA QUERCIFOLIA;

CASEIN; CHROMATOGRAPHIC ANALYSIS; ENZYME ACTIVITY; HYDROLASES; ION CHROMATOGRAPHY; ION EXCHANGE; LATEXES;

AMINO ACIDS;

CARICA PAPAYA; CARICACEAE; VASCONCELLEA QUERCIFOLIA;

CYSTEINE PROTEINASE; INSULIN; LATEX; VEGETABLE PROTEIN;

AMINO ACID SEQUENCE; BIOCATALYSIS; CARICACEAE; CHEMISTRY; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; ISOELECTRIC POINT; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS;

AMINO ACID SEQUENCE; BIOCATALYSIS; CARICACEAE; CYSTEINE ENDOPEPTIDASES; ENZYME STABILITY; INSULIN; ISOELECTRIC POINT; LATEX; MOLECULAR SEQUENCE DATA; PLANT PROTEINS; SUBSTRATE SPECIFICITY;

EID: 78049251906     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf904295x     Document Type: Article

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