Methods to detect cell surface expression and constitutive activity of GPR6

Methods in Enzymology

Volumn 484, Issue C, 2010, Pages 179-195

  Prasad, Balakrishna M ^a   Hollins, Bettye ^b   Lambert, Nevin A ^b  

^a EISENHOWER ARMY MEDICAL CENTER   (United States)

^b MEDICAL COLLEGE OF GEORGIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL SURFACE PROTEIN; CHYMOTRYPSIN; CYCLIC AMP; CYCLIC AMP RESPONSIVE ELEMENT BINDING PROTEIN; DOPAMINE 1 RECEPTOR; EPITOPE; G PROTEIN COUPLED RECEPTOR; G PROTEIN COUPLED RECEPTOR 6; PHOSPHODIESTERASE INHIBITOR; UNCLASSIFIED DRUG;

ANTIBODY LABELING; ANTIGEN BINDING; BIOACCUMULATION; BIOLUMINESCENCE RESONANCE ENERGY TRANSFER; BIOTINYLATION; BOOK; CELL ASSAY; CELL COMPARTMENTALIZATION; CELL CULTURE; CELL LABELING; CELL MEMBRANE; CELL STRAIN HEK293; COMPARATIVE STUDY; ENZYME IMMUNOASSAY; HETEROLOGOUS EXPRESSION; HUMAN; MOLECULAR IMAGING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN DETERMINATION; PROTEIN EXPRESSION; SENSITIVITY ANALYSIS;

EID: 78049243428     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/B978-0-12-381298-8.00010-1     Document Type: Chapter

References (19)

1. Calebiro D., Nikolaev V.O., Gagliani M.C., de Filippis T., Dees C., Tacchetti C., Persani L., Lohse M.J. Persistent cAMP-signals triggered by internalized G-protein-coupled receptors. PLoS Biol. 2009, 7:e1000172.
2. Eggerickx D., Denef J.F., Labbe O., Hayashi Y., Refetoff S., Vassart G., Parmentier M., Libert F. Molecular cloning of an orphan G-protein coupled receptor that constitutively activates adenylate cyclase. Biochem. J. 1995, 309:837-843.
3. Ferguson S.S. Evolving concepts in G protein-coupled receptor endocytosis: The role in receptor desensitization and signaling. Pharmacol. Rev. 2001, 53:1-24.
4. Gottardi C.J., Caplan M.J. Cell surface biotinylation in the determination of epithelial membrane polarity. J. Tissue Cult. Methods 1992, 14:173-180.
5. 2+ release by activating the sphingosine-kinase pathway. Biochem. Biophys. Res. Commun. 2003, 311:329-336.
6. Jalink K., Moolenaar W.H. G protein-coupled receptors: The inside story. Bioessays 2010, 32:13-16.
7. Jiang L.I., Collins J., Davis R., Lin K.-M., DeCamp D., Roach T., Hsueh R., Rebres R.A., Ross E.M., Taussig R., Fraser I., Sternweis P.C. Use of a cAMP BRET sensor to characterize a novel regulation of cAMP by the sphingosine 1-phosphate/G13 pathway. J. Biol. Chem. 2007, 282:10576-10584.
8. Lobo M.K., Cui Y., Ostlund S.B., Balleine B.W., Yang X.W. Genetic control of instrumental conditioning by striatopallidal neuron-specific S1P receptor GPR6. Nat. Neurosci. 2007, 10:1395-1397.
9. Mehlmann L.M., Saeki Y., Tanaka S., Brennan T.J., Evsikov A.V., Pendola F.L., Knowles B.B., Eppig J.J., Jaffe L.A. The Gs-linked receptor GPR3 maintains meiotic arrest in mammalian oocytes. Science 2004, 306:1947-1950.
10. Nikolaev V.O., Lohse M.J. Monitoring of cAMP synthesis and degradation in living cells. Physiology (Bethesda) 2006, 21:86-92.
11. Padmanabhan S., Lambert N.A., Prasad B.M. Activity-dependent regulation of the dopamine transporter is mediated by Ca(2+)/calmodulin-dependent protein kinase signaling. Eur. J. Neurosci. 2008, 28:2017-2027.
12. Padmanabhan S., Myers A.G., Prasad B.M. Constitutively active GPR6 is located in the intracellular compartments. FEBS Lett. 2009, 583:107-112.
13. Sankaranarayanan S., De Angelis D., Rothman J.E., Ryan T.A. The use of pHluorins for optical measurements of presynaptic activity. Biophys. J. 2000, 79:2199-2208.
14. Seifert R., Wenzel-Seifert K. Constitutive activity of G-protein-coupled receptors: Cause of disease and common property of wild-type receptors. Naunyn Schmiedeberg's Arch. Pharmacol. 2002, 366:381-416.
15. Song Z.H., Young W.S., Brownstein M.J., Bonner T.I. Molecular cloning of a novel candidate G protein-coupled receptor from rat brain. FEBS Lett. 1994, 12:375-379.
16. Tanaka S., Ishii K., Kasai K., Yoon S.O., Saeki Y.J. Neural expression of G-protein coupled receptors GPR3, GPR6, and GPR12 up-regulates cyclic AMP levels and promotes neurite outgrowth. J. Biol. Chem. 2007, 282:10506-10515.
17. Thathiah A., Spittaels K., Hoffmann M., Staes M., Cohen A., Horré K., Vanbrabant M., Coun F., Baekelandt V., Delacourte A., Fischer D.F., Pollet D., et al. The orphan G protein-coupled receptor 3 modulates amyloid-beta peptide generation in neurons. Science 2009, 323:946-951.
18. Uhlenbrock K., Gassenhuber H., Kostenis E. Sphingosine 1-phosphate is a ligand of the human gpr3, gpr6 and gpr12 family of constitutively active G-protein coupled receptors. Cell. Signal. 2002, 14:941-953.
19. Yin H., Chu A., Li W., Wang B., Shelton F., Otero F., Nguyen D.G., Caldwell J.S., Chen Y.A. Lipid G protein-coupled receptor ligand identification using beta-arrestin PathHunter assay. J. Biol. Chem. 2009, 284:12328-12338.