High stability and fast recovery of expression of the TOL plasmid-carried toluene catabolism genes of pseudomonas putida mt-2 under conditions of oxygen limitation and oscillation

Applied and Environmental Microbiology

Volumn 76, Issue 20, 2010, Pages 6715-6723

  MartÍnez Lavanchy, Paula M ^a   Müller, Christina ^a   Nijenhuis, Ivonne ^a   Kappelmeyer, Uwe ^a   Buffing, Marieke ^a   McPherson, Kyle ^a   Heipieper, Hermann J ^a  

^a HELMHOLTZ CENTRE FOR ENVIRONMENTAL RESEARCH UFZ   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

A-CARBON; CATABOLIC GENES; CO-SUBSTRATE; FAST RECOVERY; GENES ENCODING; HIGH STABILITY; META-CLEAVAGE PATHWAY; OXYGEN CONCENTRATIONS; OXYGEN DEPLETION; OXYGEN LIMITATION; PSEUDOMONAS PUTIDA; REVERSE TRANSCRIPTION; TOL PLASMID; TRICARBOXYLIC ACID CYCLE;

ACIDS; BACTERIA; BIODEGRADATION; CATALYSTS; DEGRADATION; ENCODING (SYMBOLS); ENZYME ACTIVITY; ENZYMES; GENES; MICROBIOLOGY; OXYGEN; TOLUENE;

TRANSCRIPTION;

BACTERIAL PROTEIN; BENZOIC ACID DERIVATIVE; CARBOXYLIC ACID; OXYGEN; TOLUENE;

ANOXIC CONDITIONS; BACTERIUM; BIODEGRADATION; CATABOLISM; ENZYME ACTIVITY; ESTER; GENE EXPRESSION; GENETIC ANALYSIS; OXYGEN; PLASMID; POLYMERASE CHAIN REACTION; TOLUENE;

AEROBIC METABOLISM; ANIMAL; ARTICLE; BIOSYNTHESIS; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; OXIDATION REDUCTION REACTION; PLASMID; PSEUDOMONAS PUTIDA; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

AEROBIOSIS; ANIMALS; BACTERIAL PROTEINS; BENZOATES; CARBOXYLIC ACIDS; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, BACTERIAL; METABOLIC NETWORKS AND PATHWAYS; OXIDATION-REDUCTION; OXYGEN; PLASMIDS; PSEUDOMONAS PUTIDA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TOLUENE;

BACTERIA (MICROORGANISMS); PSEUDOMONAS PUTIDA;

EID: 77958519731     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01039-10     Document Type: Article

References (34)

1. Agteren, M. H. V., S. Keuning, and D. B. Janssen. 1998. Handbook on biodegradation and biological treatment of hazardous organic compounds, p. 243-255. Kluwer Academic Publishers, Dordrecht, Netherlands.
2. Arras, T., J. Schirawski, and G. Unden. 1998. Availability of O2as a sub-conditions. J. Bacteriol. 180:2133-2136.
3. Assinder, S. J., and P. A. Williams. 1990. The TOL plasmids: determinants of the catabolism of toluene and the xylenes. Adv. Microb. Physiol. 31:1-69.
4. Balcke, G. U., S. Wegener, B. Kiesel, D. Benndorf, M. Schlomann, and C. Vogt. 2008. Kinetics of chlorobenzene biodegradation under reduced oxygen levels. Biodegradation 19:507-518.
5. Baldwin, B. R., C. H. Nakatsu, and L. Nies. 2003. Detection and enumeration of aromatic oxygenase genes by multiplex and real-time PCR. Appl. Environ. Microbiol. 69:3350-3358.
6. Brune, A., P. Frenzel, and H. Cypionka. 2000. Life at the oxic-anoxic interface: microbial activities and adaptations. FEMS Microbiol. Rev. 24:691-710.
7. Dominguez-Cuevas, P., J. E. Gonzalez-Pastor, S. Marques, J. L. Ramos, and V. de Lorenzo. 2006. Transcriptional tradeoff between metabolic and stressresponse programs in Pseudomonas putida KT2440 cells exposed to toluene. J. Biol. Chem. 281:11981-11991.
8. Duetz, W. A., C. de Jong, P. A. Williams, and J. G. van Andel. 1994. Competition in chemostat culture between Pseudomonas strains that use different pathways for the degradation of toluene. Appl. Environ. Microbiol. 60:2858-2863.
9. Duetz, W. A., S. Marques, B. Wind, J. L. Ramos, and J. G. van Andel. 1996. Catabolite repression of the toluene degradation pathway in Pseudomonas putida harboring pWWO under various conditions of nutrient limitation in chemostat culture. Appl. Environ. Microbiol. 62:601-606.
10. Duetz, W. A., B. Wind, M. Kamp, and J. G. van Andel. 1997. Effect of growth rate, nutrient limitation and succinate on expression of TOL pathway enzymes in response to m-xylene in chemostat cultures of Pseudomonas putida (pWWO). Microbiology 143:2331-2338.
11. Kukor, J. J., and R. H. Olsen. 1996. Catechol 2,3-dioxygenases functional in oxygen-limited (hypoxic) environments. Appl. Environ. Microbiol. 62:1728-1740.
12. Leahy, J. G., and R. H. Olsen. 1997. Kinetics of toluene degradation by toluene-oxidizing bacteria as a function of oxygen concentration, and the effect of nitrate. FEMS Microbiol. Ecol. 23:23-30.
13. Livak, K. J., and T. D. Schmittgen. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2δ CT method. Methods 25:402-408.
14. 38 depending on the growth phase. Mol. Microbiol. 31:1105-1113.
15. Nakai, C., H. Kagamiyama, M. Nozaki, T. Nakazawa, S. Inouye, Y. Ebina, and A. Nakazawa. 1983. Complete nucleotide sequence of the metapyrocatechase gene on the TOI plasmid of Pseudomonas putida mt-2. J. Biol. Chem. 258:2923-2928.
16. Nelson, K. E., C. Weinel, I. T. Paulsen, R. J. Dodson, H. Hilbert, V. A. Martins dos Santos, D. E. Fouts, S. R. Gill, M. Pop, M. Holmes, L. Brinkac, M. Beanan, R. T. DeBoy, S. Daugherty, J. Kolonay, R. Madupu, W. Nelson, O. White, J. Peterson, H. Khouri, I. Hance, P. C. Lee, E. Holtzapple, D. Scanlan, K. Tran, A. Moazzez, T. Utterback, M. Rizzo, K. Lee, D. Kosack, D. Moestl, H. Wedler, J. Lauber, D. Stjepandic, J. Hoheisel, M. Straetz, S. Heim, C. Kiewitz, J. A. Eisen, K. N. Timmis, A. Dusterhoft, B. Tummler, and C. M. Fraser. 2002. Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440. Environ. Microbiol. 4:799-808.
17. Neumann, G., S. Cornelissen, F. van Breukelen, S. Hunger, H. Lippold, N. Loffhagen, L. Y. Wick, and H. J. Heipieper. 2006. Energetics and surface properties of Pseudomonas putida DOT-T1E in a two-phase fermentation system with 1-decanol as second phase. Appl. Environ. Microbiol. 72:4232-4238.
18. Olsen, R. H., M. D. Mikesell, J. J. Kukor, and A. M. Byrne. 1995. Physiological attributes of microbial BTEX degradation in oxygen-limited environments. Environ. Health Perspect 103(Suppl. 5):49-51.
19. Parales, R. E., J. V. Parales, D. A. Pelletier, and J. L. Ditty. 2008. Diversity of microbial toluene degradation pathways. Adv. Appl. Microbiol. 64:1-73.
20. Pfaffl, M. W. 2001. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29:e45.
21. Ramos, J. L., S. Marques, and K. N. Timmis. 1997. Transcriptional control of the Pseudomonas TOL plasmid catabolic operons is achieved through an interplay of host factors and plasmid-encoded regulators. Annu. Rev. Microbiol. 51:341-373.
22. Rosell, M., S. Finsterbusch, S. Jechalke, T. Hubschmann, C. Vogt, and H. H. Richnow. 2010. Evaluation of the effects of low oxygen concentration on stable isotope fractionation during aerobic MTBE biodegradation. Environ. Sci. Technol. 44:309-315.
23. Saleh-Lakha, S., M. Miller, R. G. Campbell, K. Schneider, P. Elahimanesh, M. M. Hart, and J. T. Trevors. 2005. Microbial gene expression in soil: methods, applications and challenges. J. Microbiol. Methods 63:1-19.
24. Shaler, T. A., and G. M. Klecka. 1986. Effects of dissolved oxygen concentration on biodegradation of 2,4-dichlorophenoxyacetic acid. Appl. Environ. Microbiol. 51:950-955.
25. Shaw, J. P., and S. Harayama. 1992. Purification and characterisation of the NADH:acceptor reductase component of xylene monooxygenase encoded by the TOL plasmid pWWO of Pseudomonas putida mt-2. Eur. J. Biochem. 209:51-61.
26. Suzuki, M., T. Hayakawa, J. P. Shaw, M. Rekik, and S. Harayama. 1991. Primary structure of xylene monooxygenase: similarities to and differences from the alkane hydroxylation system. J. Bacteriol. 173:1690-1695.
27. Tschech, A., and G. Fuchs. 1987. Anaerobic degradation of phenol by pure cultures of newly isolated denitrifying pseudomonads. Arch. Microbiol. 148: 213-217.
28. Ugidos, A., G. Morales, E. Rial, H. D. Williams, and F. Rojo. 2008. The coordinate regulation of multiple terminal oxidases by the Pseudomonas putida ANR global regulator. Environ. Microbiol. 10:1690-1702.
29. Velazquez, F., V. de Lorenzo, and M. Valls. 2006. The m-xylene biodegradation capacity of Pseudomonas putida mt-2 is submitted to adaptation to abiotic stresses: evidence from expression profiling of xyl genes. Environ. Microbiol. 8:591-602.
30. Velazquez, F., V. Parro, and V. de Lorenzo. 2005. Inferring the genetic network of m-xylene metabolism through expression profiling of the xyl genes of Pseudomonas putida mt-2. Mol. Microbiol. 57:1557-1569.
31. Wiessner, A., U. Kappelmeyer, P. Kuschk, and M. Kastner. 2005. Influence of the redox condition dynamics on the removal efficiency of a laboratoryscale constructed wetland. Water Res. 39:248-256.
32. Williams, P. A., and K. Murray. 1974. Metabolism of benzoate and the methylbenzoates by Pseudomonas putida (arvilla) mt-2: evidence for the existence of a TOL plasmid. J. Bacteriol. 120:416-423.
33. Wilson, L. P., and E. J. Bouwer. 1997. Biodegradation of aromatic compounds under mixed oxygen/denitrifying conditions: a review. J. Ind. Microbiol. Biotechnol. 18:116-130.
34. Worsey, M. J., and P. A. Williams. 1975. Metabolism of toluene and xylenes by Pseudomonas putida (arvilla) mt-2: evidence for a new function of the TOL plasmid. J. Bacteriol. 124:7-13.