CaMKII phosphorylates serine 10 of p27 and confers apoptosis resistance to HeLa cells

Biochemical and Biophysical Research Communications

Volumn 401, Issue 3, 2010, Pages 350-355

  Kajihara, Ryutaro ^a   Fukushige, Shota ^a   Shioda, Norifumi ^b   Tanabe, Kano ^a   Fukunaga, Kohji ^b   Inui, Seiji ^a  

^a KUMAMOTO UNIVERSITY   (Japan)

^b TOHOKU UNIVERSITY   (Japan)

Author keywords

Apoptosis; CaMKII; P27; Protein phosphatase 6

Indexed keywords

CALCIUM CALMODULIN DEPENDENT PROTEIN KINASE II; CALCIUM IONOPHORE; CYCLOHEXIMIDE; PHOSPHOPROTEIN PHOSPHATASE; PHOSPHOPROTEIN PHOSPHATASE 6; PROTEIN BCL XL; PROTEIN P27; SERINE; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; CELL SURVIVAL; CONTROLLED STUDY; FLOW CYTOMETRY; GENETIC TRANSFECTION; HELA CELL; HUMAN; HUMAN CELL; MUTATION; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SEQUENCE ANALYSIS; TRANSIENT EXPRESSION;

AMINO ACID SEQUENCE; APOPTOSIS; BCL-X PROTEIN; CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASE TYPE 2; CYCLIN-DEPENDENT KINASE INHIBITOR P27; DNA, COMPLEMENTARY; HELA CELLS; HUMANS; MOLECULAR SEQUENCE DATA; MUTATION; PHOSPHOPROTEIN PHOSPHATASES; PHOSPHORYLATION; SERINE;

EID: 77958153958     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2010.09.051     Document Type: Article

References (32)

1. Sutton A., Immanuel D., Arndt K.T. The SIT4 protein phosphatase functions in late G1 for progression into S Phase. Mol. Cell. Biol. 1991, 11:2133-2148.
2. Bastians H., Ponsting H. The novel human protein serine/threonine phosphatase 6 is a functional homologue of budding yeast Sit4p and fission yeast pp1, which are invloved in cell cycle regulation. J. Cell Sci. 1996, 109:2865-2874.
3. Becker W., Kentrup H., Klumpp S., Schultz J.E., Joost H.G. Molecular cloning of a protein serine/threonine phosphatase containing a putative regulatory tetratricopeptide repeat domain. J. Biol. Chem. 1994, 269:22586-22592.
4. MacKeigan J.P., Murphy L.O., Blenis J. Sensitized RNAi screen of human kinases and phosphatases identifies new regulators of apoptosis and chemoresistance. Nat. Cell Biol. 2005, 7:591-600.
5. Inui S., Sanjo H., Maeda K., Yamamoto H., Miyamoto E., Sakaguchi N. Ig receptor binding protein 1 (α4) is associated with a rapamycin-sensitive signal transduction in lymphocytes through direct binding to the catalytic subunit of protein phosphatase 2A. Blood 1998, 92:539-546.
6. Murata K., Wu J., Brautigan D.L. B cell receptor-associated protein α4 displays rapamycin-sensitive binding directly to the catalytic subunit of protein phosphatase 2A. Proc. Nat. Acad. Sci. USA 1997, 94:10624-10629.
7. Di Como C.J., Arndt K.T. Nutrients, via the Tor proteins, stimulate the association of Tap42 with type 2A phosphatases. Genes Dev. 1996, 10:1904-1916.
8. Chen J., Peterson R.T., Schreiber S.L. α4 associates with protein phosphatases 2A, 4, and 6. Biochem. Biophys. Res. Commun. 1998, 247:827-832.
9. Inui S., Maeda K., Hua D.R., Yamashita T., Yamamoto H., Miyamoto E., Sakaguchi N. BCR signal through α4 is involved in S6 kinase activation and required for B cell maturation including isotype switching and V region somatic hypermutation. Int. Immunol. 2002, 14:177-187.
10. Hua D.R., Inui S., Yamashita T., Maeda K., Takagi K., Takeda J., Sakaguchi N. T cell-specific gene targeting reveals that α4 is required for early T cell development. Eur. J. Immunol. 2003, 33:1899-1906.
11. Kong M., Fox C.J., Mu J., Solt L., Xu A., Cinalli R.M., Birnbaum M.J., Lindsten T., Thompson C.B. The PP2A-associated protein α4 is an essential inhibitor of apoptosis. Science 2004, 306:695-698.
12. Yamashita T., Inui S., Maeda K., Hua D.R., Takagi K., Fukunaga K., Sakaguchi N. Regulation of CaMKII by α4/PP2Ac contributes to learning and memory. Brain Res.. 2006, 1082:1-10.
13. Griffith L.C. Calcium/calmodulin-dependent protein kinase II: An unforgettable kinase. J. Neurosci. 2004, 8391-8393.
14. Strack S., Barban M.A., Wadzinski B.E., Colbran R.J. Differential inactivation of postsynaptic density-associated and soluble Ca2+ /calmodulin-dependent protein kinase II by protein phosphatase 1 and 2A. J. Neurochem. 1997, 68:92119-92128.
15. Braun A.P., Schulman H. The multifunctional calcium/calmodulin-dependent protein kinase: from form to function. Annu. Rev. Physiol. 1995, 57:417-445.
16. Wright S.C., Schellenberger U., Ji L., Wang H., Larrick J.W. Calmodulin-dependent protein kinase II mediates signal transduction in apoptosis. FASEB J. 1997, 11:843-849.
17. Zhang T., Brown J.H. Role of Ca2+/calmodulin-dependent protein kinase II in cardiac hypertrophy and heart failure. Cardiovasc. Res. 2004, 63:476-486.
18. Rokhlin O., Taghiyev A.F., Bayer K.U., Bumcrot D., Kotelianski V.E., Glover R.A., Cohen M.B. Calcium/calmodulin-dependent kinase II plays an important role in prostate cancer cell survival. Cancer Biol.Ther. 2007, 6:732-742.
19. Mishra S., Mishra J.P., Gee K., McManus D.C., LaCasse E.C., Kumar A. Distinct role of calmodulin and calmodulin-dependent protein kinase-II in lipopolysaccharide and tumor necrosis factor-α-mediated suppression of apoptosis and antiapoptotic c-IAP2 gene. J. Biol. Chem. 2005, 280:37536-37546.
20. Yang B.F., Xiao C., Roa W.H., Krammer P.H., Hao C. Calcium/calmodulin-dependent protein kinase II regulation of c-FLIP expression and phosphorylation in modulation of Fas-mediated signaling in malignant glioma cells. J. Biol. Chem. 2003, 278:7043-7050.
21. Guan X., Chen L., Wang J., Geng H., Chu X., Zhang Q., Du L., De W. Mutations of phosphorylation sites Ser10 and Thr187 of p27Kip1 abolish cytoplasmic redistribution but do not abrogate G0/1 phase arrest in the HepG2 cell line. Biochem. Biophys. Res. Commun. 2006, 347:601-607.
22. Fujita N., Sato S., Katayama K., Tsuro T. Akt-dependent phosphorylation of p27Kip promotes binding to 14-3-3 and cytoplasmic localization. J. Biol. Chem. 2002, 277:28706-28713.
23. Conqueret O. New roles for p21 and p27 cell-cycle inhibitors: a function for each cell compartment?. Trends Cell Biol. 2003, 13:65-70.
24. Fukunaga K., Sato H., Takatsu K., Tominaga A., Miyamoto E. Monoclonal antibody against a multifunctional calmodulin-dependent protein kinase from rat brain and the tissue distribution of the enzyme. Biomed. Res. 1986, 7:405-413.
25. Fukunaga K., Yamamoto H., Matsui K., Higashi K., Miyamoto E. Purification and characterization of a Ca2+- and calmodulin-dependent protein kinase from rat brain. Eur. J. Neurochem. 1982, 39:1607-1617.
26. Evans D.R.H., Myles T., Hofsteenge J., Hemmings B.A. Functional expression of human PP2Ac in yeast permits the identification of novel C-terminal and dominant-negative mutant forms. J. Biol. Chem. 1999, 274:24038-24046.
27. Cho U.S., Xu W. Crystal structure of a protein phosphatase 2A heterotrimeric holoenzyme. Nature 2007, 445:53-57.
28. Ishida N., Kitagawa M., Hatakeyama S., Nakayama K. Phosphorylation at serine 10, a major phosphorylation site of p27kip1, increases its protein stability. J. Biol. Chem. 2000, 275:25146-25154.
29. Wallach D., Varfolomeev E.E., Malinin N.L., Golstef Y.G., Kovalenko A.V., Boldin M.P. Tumor necrosis factor receptor and Fas signaling mechanisms. Annu. Rev. Immunol. 1999, 17:331-367.
30. Sherr C.J., Roberts J.M. CDK inhibitors: positive and negative regulators of G1-phase progression. Genes Dev. 1999, 13:1501-1512.
31. Boehm M., Yoshimoto T., Crook M.F., Nallamshetty S., True A., Nabel G.J., Nabel E.G. A growth factor-dependent nuclear kinase phosphorylates p27Kip1 and regulates cell cycle progression. EMBO 2002, 21:3390-3401.
32. Janumyan Y., Cui Q., Yan L., Sansam C.G., Valentin M., Yang E. G0 function of Bcl2 and Bcl-xL requires Bax, Bak, and p27 phosphorylation by Mirk, revealing a novel role of Bax and Bak in quiescence regulation. J. Biol. Chem. 2008, 283:34108-34120.