Functional analysis of genes in Aedes aegypti embryos

Cold Spring Harbor Protocols

Volumn 5, Issue 10, 2010, Pages

  Clemons, Anthony ^a,b   Haugen, Morgan ^a   Severson, David ^b   Duman Scheel, Molly ^a,b  

^a INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF NOTRE DAME   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

SMALL INTERFERING RNA;

AEDES AEGYPTI; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; EMBRYO; EMBRYO DEVELOPMENT; FEMALE; GENE EXPRESSION REGULATION; GENE FUNCTION; GENE SILENCING; GENE TARGETING; GENOME ANALYSIS; INSECT DEVELOPMENT; INSECT GENOME; MICROINJECTION; NONHUMAN; RNA INTERFERENCE;

AEDES AEGYPTI;

EID: 77958099592     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5511     Document Type: Article

References (9)

1. Blitzer EJ, Vyazunova I, Lan Q. 2005. Functional analysis of AeSCP-2 using gene expression knockdown in the yellow fever mosquito, Aedes aegypti. Insect Mol Biol 14: 301-307.
2. Clemons A, Mori A, Haugen M, Severson D, Duman-Scheel M. 2010a. Culturing and egg collection of Aedes aegypti. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5507.
3. Clemons A, Flannery E, Kast K, Severson D, Duman-Scheel M. 2010b. Immunohistochemical analysis of protein expression during Aedes aegypti development. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5510.
4. Clemons A, Haugen M, Flannery E, Tomchaney M, Kast K, Jacowski C, Le C, Mori A, Simanton Holland W, Sarro J et al. 2010c. Aedes aegypti: An emerging model for vector mosquito development. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.emo141.
5. Haugen M, Tomchaney M, Kast K, Flannery E, Clemons A, Jacowski C, Simanton Holland W, Le C, Severson D, Duman-Scheel M. 2010. Whole-mount in situ hybridization for analysis of gene expression during Aedes aegypti development. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5509.
6. Jasinskiene N, Juhn J, James AA. 2007. Microinjection of A. aegypti embryos to obtain transgenic mosquitoes. J Vis Exp 5: 219. doi: 10.3791/219.
7. Lobo NF, Clayton JR, Fraser MJ, Kafatos FC, Collins FH. 2006. High efficiency germ-line transformation of mosquitoes. Nat Protoc 1: 1312-1317.
8. Luna BM, Juhn J, James AA. 2007. Injection of dsRNA into female A. aegypti mosquitos. J Vis Exp 5: 215. doi: 10.3791/215.
9. Morlais I, Mori A, Schneider JR, Severson DW. 2003. A targeted approach to the identification of candidate genes determining susceptibility to Plasmodium gallinaceum in Aedes aegypti. Mol Genet Genomics 269: 753-764.