Whole-mount in situ hybridization for analysis of gene expression during Aedes aegypti development

Cold Spring Harbor Protocols

Volumn 5, Issue 10, 2010, Pages

  Haugen, Morgan ^a   Tomchaney, Michael ^a,b   Kast, Kristopher ^b   Flannery, Ellen ^b   Clemons, Anthony ^a,b   Jacowski, Caitlin ^a,b   Holland, Wendy Simanton ^a   Le, Christy ^b   Severson, David ^b   Duman Scheel, Molly ^a,b  

^a INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF NOTRE DAME   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AEDES AEGYPTI; ANIMAL TISSUE; ARTHROPOD LARVA; ARTICLE; CONTROLLED STUDY; DEVELOPMENTAL GENE; EMBRYO; EMBRYO DEVELOPMENT; GENE EXPRESSION REGULATION; GENE FUNCTION; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; INSECT DEVELOPMENT; NONHUMAN; PROTEIN EXPRESSION; TISSUE FIXATION; TISSUE PREPARATION;

AEDES AEGYPTI;

EID: 77958093959     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5509     Document Type: Article

References (8)

1. Clemons A, Haugen M, Flannery E, Kast K, Jacowski C, Severson D, Duman-Scheel M. 2010a. Fixation and preparation of developing tissues from Aedes aegypti. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5508.
2. Clemons A, Flannery E, Kast K, Severson D, Duman-Scheel M. 2010b. Immunohistochemical analysis of protein expression during Aedes aegypti development. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5510.
3. Clemons A, Haugen M, Flannery E, Tomchaney M, Kast K, Jacowski C, Le C, Mori A, Simanton Holland W, Sarro J et al. 2010c. Aedes aegypti: An emerging model for vector mosquito development. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.emo141.
4. Duman-Scheel M, Pirkl N, Patel NH. 2002. Analysis of the expression pattern of Mysidium columbiae wingless provides evidence for conserved mesodermal and retinal patterning processes among insects and crustaceans. Dev Genes Evol 212: 114-123.
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6. Patel NH, Hayward DC, Lall S, Pirkl NR, DiPietro D, Ball EE. 2001. Grasshopper hunchback expression reveals conserved and novel aspects of axis formation and segmentation. Development 128: 3459-3472.
7. Tautz D, Pfeifle C. 1989. A non-radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback. Chromosoma 98: 81-85.
8. Van Zomeren-Dohm A, Flannery E, Duman-Scheel M. 2008. Whole mount in situ hybridization detection of mRNA in GFP-marked Drosophila imaginal disc mosaic clones. Fly 2: 323-325.