High efficiency germ-line transformation of mosquitoes

Nature Protocols

Volumn 1, Issue 3, 2006, Pages 1312-1317

  Lobo, Neil F ^a   Clayton, John R ^b   Fraser, Malcolm J ^a   Kafatos, Fotis C ^c   Collins, Frank H ^a  

^a UNIVERSITY OF NOTRE DAME   (United States)

^b JOHNS HOPKINS BLOOMBERG SCHOOL OF PUBLIC HEALTH   (United States)

^c IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL; ARTICLE; CYTOLOGY; GENETIC PROCEDURES; GENETIC TRANSFORMATION; GENETICS; GERM CELL; MICROINJECTION; MOSQUITO; TRANSGENE; TRANSGENIC ANIMAL;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; CULICIDAE; GENETIC TECHNIQUES; GERM CELLS; MICROINJECTIONS; TRANSFORMATION, GENETIC; TRANSGENES;

EID: 34347273800     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2006.221     Document Type: Article

References (37)

1. Morris, A.C. in The Molecular Biology of Insect Vectors of Disease (eds. Crampton, J.M., Beard, C.B. & Louis, C.) 423-429 (Chapman & Hall, London, 1997).
2. Grossman, G.L. et al. Germline transformation of the malaria vector, Anopheles gambiae, with the piggyBac transposable element. Insect Mol. Biol. 10, 597-604 (2001).
3. Catteruccia, F. et al. Stable germline transformation of the malaria mosquito Anopheles stephensi. Nature 405, 959-962 (2000).
4. Nolan, T., Bower, T.M., Brown, A.E., Crisanti, A. & Catheruccia, F. piggyBac-mediated germline transformation of the malaria mosquito Anopheles stephensi using the red fluorescent protein dsRED as a selectable marker. J. Biol. Chem. 277, 8759-8762 (2002).
5. Lobo, N.F., Hua-Van, A., Li, X., Nolen, B.M. & Fraser, M.J., Jr. Germ Line transformation of the yellow fever mosquito, Aedes aegypti, mediated by transpositional insertion of a piggyBac vector. Insect Mol. Biol. 11, 133-139 (2002).
6. Coates, C.J., Jasinskiene, N., Miyashiro, L. & James, A.A. Mariner transposition and transformation of the yellow fever mosquito, Aedes aegypti. Proc. Natl. Acad. Sci. USA 95, 3748-3751 (1998).
7. Perera, O.P., Harrell, II R.A. & Handler, A.M. Germ-line transformation of the South American malaria vector, Anopheles albimanus, with a piggyBac/EGFP transposon vector is routine and highly efficient. Insect Mol. Biol. 11, 291-297 (2002).
8. Allen, M.L., O'Brochta, D.A., Atkinson, P.W. & Levesque, C.S. Stable, germ-line transformation of Culex quinquefasciatus (Diptera: Culicidae). J. Med. Entomol. 38, 701-710 (2001).
9. Fraser, M.J., Ciszczon, T., Elick, T. & Bauser, C. Precise excision of TTAA-specific lepidopteran transposons piggyBoc (IFP2) and tagalong (TFP3) from the baculovirus genome in cell lines from two species of Lepidoptera. Insect Mol. Biol. 5, 141-151 (1996).
10. Jasinskiene, N. et al. Stable transformation of the yellow fever mosquito, Aedes aegypti, with the Hermes element from the housefly. Proc. Natl. Acad. Sci. USA 95, 3743-3747 (1998).
11. O'Brochta, D.A. et al. Gene vector and transposable element behavior in mosquitoes. J. Exp. Biol. 206, 3823-3834 (2003).
12. Berghammer, A.J., Klingler, M. & Wimmer, E.A. A universal marker for transgenic insects. Nature 402, 370-371 (1999).
13. Adelman, Z.N., Jasinskiene, N. & James, A.A. Development and applications of transgenesis in the yellow fever mosquito, Aedes aegypti. Mol. Biochem. Parasitol, 121, 1-10 (2002).
14. Riehle, M.A., Srinivasan, P., Moreira, C.K. & Jacobs-Lorena, M. Towards genetic manipulation of wild mosquito populations to combat malaria: advances and challenges. J. Exp. Biol. 206, 3809-3816 (2003).
15. Horn, C. & Wimmer, E.A. A versatile vector set for animal transgenesis. Dev. Genes Evol. 210, 690-637 (2000).
16. Shin, S.W., Kokoza, V.A. & Raikhel, A.S. Transgenesis and reverse genetics of mosquito innate immunity. J. Exp. Biol. 206, 3835-3843 (2003).
17. Franz, A.W. et al. Engineering RNA interference-based resistance to dengue virus type 2 in genetically modified Aedes aegypti. Proc. Natl Acad. Sci. USA 103 4198-4203 (2006).
18. Ito, J., Ghosh, A., Moreira, L.A., Wimmer, E.A. & Jacobs-Lorena, M. Transgenic anopheline mosquitoes impaired in transmission of a malaria parasite. Nature 417, 452-455 (2002).
19. Travanty, E.A. et al. Using RNA interference to develop dengue virus resistance in genetically modified Aedes aegypti. Insect Biochem. Mol. Biol. 34, 607-613 (2004).
20. Brown, A.E. & Catteruccia, F. Toward silencing the burden of malaria: progress and prospects for RNAi-based approaches. Biotechniques 40, Suppl: 38-44 (2006).
21. Sanchez-Vargas, I. et al. RNA interference, arthropod-bome viruses, and mosquitoes. Virus Res. 102, 65-74 (2004).
22. Ito, K., Sass, H., Urban, J., Hofbauer, A. & Schneuwly, S. GAL4-responsive UAS-tau as a tool for studying the anatomy and development of the Drosophila central nervous system. Cell Tissue Res. 290, 1-10 (1997).
23. O'Kane, C. & Gehring, W.J. Detection in situ of genomic regulatory elements in Drosophila. Proc. Natl Acad. Sci. USA 84, 9123-9127 (1987).
24. Catteruccia, F., Benton, J.P. & Crisanti, A. An Anopheles transgenic sexing strain for vector control. Nature Biotechnol. 23, 1414-1417 (2005).
25. Marrelli, M.T., Moreira, C.K., Kelly, D., Alphey, L. & Jacobs-Lorena, M. Mosquito transgenesis: What is the fitness cost? Trends Parasitol. 22, 197-202 (2006).
26. Catteruccia, F., Godfray, H.C. & Crisanti, A. Impact of genetic manipulation on the fitness of Anopheles stephensi mosquitoes. Science 299, 1225-1227 (2003).
27. Moreira, L.A., Wang, J., Collins, F.H. & Jacobs-Lorena, M. Fitness of anopheline mosquitoes expressing transgenes that inhibit Plasmodium development. Genetics 166, 1337-1341 (2004).
28. Zhong, D. et al. Dynamics of gene introgressión in the African malaria vector Anopheles gambiae. Genetics 172, 2359-2365 (2006).
29. Menge, D.M. et al. Fitness consequences of Anopheles gambiae population hybridization. Malar. J. 4, 44 (2005).
30. Rasgon, J.L. & Gould, F. Transposable element insertion location bias and the dynamics of gene drive in mosquito populations. Insect Mol. Biol. 14, 493-500 (2005).
31. Li, J. Simple mathematical models for interacting wild and transgenic mosquito populations. Math. Biosci. 189, 39-59 (2004).
32. Thomas, D.D., Donnelly, C.A., Wood, R.J. & Alphey, L.S. Insect population control using a dominant, repressible, lethal genetic system. Science 287, 2474-2476 (2000).
33. Sambrook, J., Fritsch, E.F. & Maniatis, T. in Molecular Cloning: A Laboratory Manual 2nd edn. (eds. Ford, N., Nolan, C. & Ferguson, M.) 1.42-1.50 (Cold Spring Harbor Laboratory Press, Cold Spring. Harbor, New York, 1989).
34. Munstermann, L.E. in The Molecular Biology of Insect Vectors of Disease (eds. Crampton, J.M., Beard, C.B. & Louis, C.) 13-20 (Chapman & Hatl, London, 1997).
35. Benedict, M.O. in The Molecular Biology of Insect Vectors of Disease (eds. Crampton, J.M., Beard, C.B. & Louis, C.) 3-12 (Chapman & Hall, London, 1997).
36. Southern, E. Southern blotting. Nature Protocols 1, 518-525 (2006).
37. Ochman, H., Gerber, A.S. & Hartl, D.L. Genetic applications of an inverse polymerase chain reaction. Genetics 120, 621-623 (1988).