Cloning and expression of functional full-length human tissue plasminogen activator in pichia pastoris

Applied Biochemistry and Biotechnology

Volumn 162, Issue 7, 2010, Pages 2037-2048

  Majidzadeh A, Keivan ^a   Khalaj, Vahid ^a   Fatemeh, Davami ^a   Mahdi, Hemayatkar ^a   Farzaneh, Barkhordari ^a   Ahmad, Adeli ^a   Mahboudi, Fereidoun ^a  

^a PASTEUR INSTITUTE OF IRAN   (Iran)

Author keywords

Amidolytic assay; Cloning; Densitometry; Expression; Human tissue plasminogen activator; Pichia pastoris; t PA; Zymography

Indexed keywords

AMIDOLYTIC ASSAYS; DENSITOMETRY; EXPRESSION; HUMAN TISSUES; PICHIA PASTORIS; T-PA; ZYMOGRAPHY;

AMINO ACIDS; CLONING; GENE ENCODING; MAMMALS; METHANOL; TISSUE; YEAST;

GENE EXPRESSION;

ALCOHOL OXIDASE; GELATIN; PLASMIN; PLASMINOGEN; TISSUE PLASMINOGEN ACTIVATOR;

AMINO TERMINAL SEQUENCE; ARTICLE; BASE PAIRING; DNA CLEAVAGE; DOWNSTREAM PROCESSING; ENZYME ACTIVITY; ENZYME RELEASE; GENE CASSETTE; GENE VECTOR; LYSIS; METHYLOTROPHIC YEAST; MOLECULAR CLONING; NONHUMAN; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; PROTEIN FOLDING; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS; WESTERN BLOTTING; ZYMOGRAPHY;

CLONING, MOLECULAR; GENE EXPRESSION; HUMANS; PICHIA; PROTEIN FOLDING; RECOMBINANT PROTEINS; TISSUE PLASMINOGEN ACTIVATOR;

MAMMALIA; PICHIA PASTORIS; PROKARYOTA; SACCHAROMYCES CEREVISIAE;

EID: 77958086775     PISSN: 02732289     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12010-010-8979-z     Document Type: Article

References (42)

1. SR Bergmann KA Fox MM Ter-Pogossian BE Sobel D Collen 1983 Clot-selective coronary thrombolysis with tissue-type plasminogen activator Science 220 4602 1181 1183 1:STN:280:DyaL3s3itlGmuw%3D%3D 10.1126/science.6602378
2. F Van de Werf SR Bergmann KA Fox H de Geest CF Hoyng BE Sobel D Collen 1984 Coronary thrombolysis with intravenously administered human tissue-type plasminogen activator produced by recombinant DNA technology Circulation 69 3 605 610 (Pubitemid 14192274)
3. F Van de Werf PA Ludbrook SR Bergmann AJ Tiefenbrunn KA Fox H de Geest M Verstraete D Collen BE Sobel 1984 Coronary thrombolysis with tissue-type plasminogen activator in patients with evolving myocardial infarction The New England Journal of Medicine 310 10 609 613 10.1056/NEJM198403083101001 (Pubitemid 14194374)
4. Goldhaber, S. Z. (2005). Pulmonary thromboembolism. In D. L. Kasper, E. Braunwald, A. S. Fauci, et al. (Eds.), Harrison's Principles of Internal Medicine (16th ed., pp. 1561-1565). New York: McGraw-Hill.
5. GP Clagett M Sobel MR Jackson GY Lip M Tangelder R Verhaeghe 2004 Antithrombotic therapy in peripheral arterial occlusive disease: the seventh ACCP conference on antithrombotic and thrombolytic therapy Chest 126 3 Suppl 609S 626S 1:CAS:528:DC%2BD2cXptV2mt74%3D 10.1378/chest.126.3-suppl.609S (Pubitemid 39297965)
6. CJ Murray AD Lopez 1997 Mortality by cause for eight regions of the world: Global Burden of Disease Study Lancet 349 9061 1269 1276 1:STN:280:DyaK2s3os12lug%3D%3D 10.1016/S0140-6736(96)07493-4
7. Fauci, A. S., Braunwald, E., Kasper, D. L., Hauser, S. L., Longo, D. L., Jameson, J. L., et al. (Eds.). (2008). In Harrison's Principles of Internal Medicine (17th ed.). New York: McGraw-Hill.
8. SZ Goldhaber DE Vaughan JE Markis AP Selwyn MF Meyerovitz J Loscalzo DS Kim CM Kessler DL Dawley GV Sharma, et al. 1986 Acute pulmonary embolism treated with tissue plasminogen activator Lancet 2 8512 886 889 1:STN:280: DyaL2s%2FhtlKksQ%3D%3D 10.1016/S0140-6736(86)90411-3
9. Graor, R. A. (1987). Tissue Plasminogen Activator. In B. E. Sobel, D. Collen, & E. B. Grossbard (Eds.), Thrombolytic therapy (pp. 171-207). New York: Marcel Dekker.
10. HK Gold JA Johns RC Leinbach T Yasuda E Grossbard R Zusman D Collen 1987 A randomized, blinded, placebo-controlled trial of recombinant human tissue-type plasminogen activator in patients with unstable angina pectoris Circulation 75 6 1192 1199 1:STN:280:DyaL2s7ovVWrtQ%3D%3D
11. D Belin JD Vassalli C Combepine F Godeau Y Nagamine E Reich HP Kocher RM Duvoisin 1985 Cloning, nucleotide sequencing and expression of cDNAs encoding mouse urokinase-type plasminogen activator European Journal of Biochemistry 148 2 225 232 1:CAS:528:DyaL2MXhs1Wnsro%3D 10.1111/j.1432-1033.1985.tb08829.x (Pubitemid 15093608)
12. AM Ross 1999 New plasminogen activators: a clinical review Clinical Cardiology 22 3 165 171 1:STN:280:DyaK1M7otlyjsQ%3D%3D 10.1002/clc.4960220303
13. ML Simoons PW Serruys M van den Brand F Bar C de Zwaan J Res FW Verheugt XH Krauss WJ Remme F Vermeer, et al. 1985 Improved survival after early thrombolysis in acute myocardial infarction. A randomised trial by the interuniversity cardiology institute in The Netherlands Lancet 2 8455 578 82 1:STN:280:DyaL2M3psV2ksQ%3D%3D 10.1016/S0140-6736(85)90584-7
14. D Pennica WE Holmes WJ Kohr RN Harkins GA Vehar CA Ward WF Bennett E Yelverton PH Seeburg HL Heyneker DV Goeddel D Collen 1983 Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli Nature 301 5897 214 221 1:CAS:528:DyaL3sXht1Smur4%3D 10.1038/301214a0 (Pubitemid 13142222)
15. SJ Szarka EG Sihota HR Habibi S Wong 1999 Staphylokinase as a plasminogen activator component in recombinant fusion proteins Applied and Environmental Microbiology 65 2 506 513 1:CAS:528:DyaK1MXpvFCltw%3D%3D
16. JB Griffiths A Electricwala 1987 Production of tissue plasminogen activators from animal cells Advances in Biochemical Engineering/Biotechnology 34 147 166 1:STN:280:DyaL2s3psFCgtw%3D%3D 10.1007/BFb0000678
17. J Qiu JR Swartz G Georgiou 1998 Expression of active human tissue-type plasminogen activator in escherichia coli Applied and Environmental Microbiology 64 12 4891 4896 1:CAS:528:DyaK1cXnvF2htrY%3D
18. E Martegani N Forlani I Mauri D Porro WD Schleuning L Alberghina 1992 Expression of high levels of human tissue plasminogen activator in yeast under the control of an inducible GAL promoter Applied Microbiology and Biotechnology 37 5 604 608 1:CAS:528:DyaK38XmtFSltLg%3D 10.1007/BF00240734
19. PH Bessette F Aslund J Beckwith G Georgiou 1999 Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm Proceedings of the National Academy of Sciences of the United States of America 96 24 13703 13708 1:CAS:528:DyaK1MXns1Oqsbk%3D 10.1073/pnas.96.24.13703
20. CE White NM Kempi EA Komives 1994 Expression of highly disulfide-bonded proteins in Pichia pastoris Structure 2 11 1003 1005 1:CAS:528: DyaK2MXivFSkurY%3D 10.1016/S0969-2126(94)00103-0 (Pubitemid 2164561)
21. JM Cregg TS Vedvick WC Raschke 1993 Recent advances in the expression of foreign genes in Pichia pastoris Biotechnology (NY) 11 8 905 910 1:CAS:528:DyaK3sXmsVajtbk%3D 10.1038/nbt0893-905 (Pubitemid 23218978)
22. KN Faber W Harder G Ab M Veenhuis 1995 Review: methylotrophic yeasts as factories for the production of foreign proteins Yeast 11 14 1331 1344 1:CAS:528:DyaK2MXpsFemsLw%3D 10.1002/yea.320111402
23. Ausubel, F. M. (1987). Current protocols in molecular biology. New York: Wiley.
24. MS Lantz P Ciborowski 1994 Zymographic techniques for detection and characterization of microbial proteases Methods in Enzymology 235 563 594 1:CAS:528:DyaK2MXhvVShsr4%3D 10.1016/0076-6879(94)35171-6
25. PA Snoek-van Beurden JW Von den Hoff 2005 Zymographic techniques for the analysis of matrix metalloproteinases and their inhibitors Biotechniques 38 1 73 83 1:CAS:528:DC%2BD2MXptV2ltA%3D%3D 10.2144/05381RV01
26. M Soleimani F Mahboudi N Davoudi A Amanzadeh M Azizi A Adeli H Rastegar F Barkhordari B Mohajer-Maghari 2007 Expression of human tissue plasminogen activator in the trypanosomatid protozoan Leishmania tarentolae Biotechnology and Applied Biochemistry 48 Pt 1 55 61 1:CAS:528:DC%2BD2sXpsFOrsro%3D
27. HP Sorensen KK Mortensen 2005 Advanced genetic strategies for recombinant protein expression in Escherichia coli Journal of Biotechnology 115 2 113 128 1:CAS:528:DC%2BD2cXhtFahsr7P 10.1016/j.jbiotec.2004.08.004 (Pubitemid 39656438)
28. LF Vallejo U Rinas 2004 Strategies for the recovery of active proteins through refolding of bacterial inclusion body proteins Microb Cell Fact 3 1 11 10.1186/1475-2859-3-11 (Pubitemid 40230524)
29. B Gasser M Saloheimo U Rinas M Dragosits E Rodriguez-Carmona K Baumann M Giuliani E Parrilli P Branduardi C Lang D Porro P Ferrer ML Tutino D Mattanovich A Villaverde 2008 Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview Microb Cell Fact 7 11 10.1186/1475-2859-7-11 (Pubitemid 351575088)
30. DB Baruah RN Dash MR Chaudhari SS Kadam 2006 Plasminogen activators: a comparison Vascul Pharmacol 44 1 1 9 1:CAS:528:DC%2BD2MXhtlWhsb%2FK 10.1016/j.vph.2005.09.003 (Pubitemid 41799584)
31. A Upshall AA Kumar MC Bailey MD Parker KP Favreau MA Lewison ML Joseph JM Maraganore GL Mcknight 1987 Secretion of active human tissuec plasminogen activator from the filamentous fungus Aspergillus nidulans Biotechnology 5 1301 1304 1:CAS:528:DyaL1cXntVWjsA%3D%3D 10.1038/nbt1287-1301
32. MG Wiebe A Karandikar GD Robson AP Trinci JL Candia S Trappe G Wallis U Rinas PM Derkx SM Madrid H Sisniega I Faus R Montijn CA van den Hondel PJ Punt 2001 Production of tissue plasminogen activator (t-PA) in Aspergillus niger Biotechnology and Bioengineering 76 2 164 174 1:CAS:528:DC%2BD3MXms1Sksbg%3D 10.1002/bit.1156 (Pubitemid 32852491)
33. Oka, M. S., Fong, K.-L. L., Carr, S. A., & Shebuski, R. (1990). Characterization and biological properties of recombinant tPA produced in Drosophila cells culture. In H. Murakami (Ed.), Trends in Animal Cell Culture Technology (pp. 161-166). Tokyo: Kodansha.
34. SA Rouf M Moo-Young Y Chisti 1996 Tissue-type plasminogen activator: characteristics, applications and production technology Biotechnology Advances 14 3 239 266 1:CAS:528:DyaK28XlvVGrt7w%3D 10.1016/0734-9750(96)00019-5 (Pubitemid 27040134)
35. S Macauley-Patrick ML Fazenda B McNeil LM Harvey 2005 Heterologous protein production using the Pichia pastoris expression system Yeast 22 4 249 270 1:CAS:528:DC%2BD2MXjsVals7k%3D 10.1002/yea.1208 (Pubitemid 40528349)
36. S Curvers L Linneman T Klauser C Wandrey R Takors 2002 Recombinant protein production with Pichia pastoris in continuous fermentation - kinetic analysis of growth and product formation Eng Life Sci 2 8 229 235 10.1002/1618-2863(20020806)2:8<229::AID-ELSC229>3.0.CO;2-9
37. GP Lin Cereghino J Lin Cereghino AJ Sunga MA Johnson M Lim MA Gleeson JM Cregg 2001 New selectable marker/auxotrophic host strain combinations for molecular genetic manipulation of Pichia pastoris Gene 263 1-2 159 169 1:CAS:528:DC%2BD3MXht1Oku7c%3D 10.1016/S0378-1119(00)00576-X (Pubitemid 32167065)
38. S Brocca C Schmidt-Dannert M Lotti L Alberghina RD Schmid 1998 Design, total synthesis, and functional overexpression of the Candida rugosa lip1 gene coding for a major industrial lipase Protein Science 7 6 1415 1422 1:CAS:528:DyaK1cXjvFGqs7Y%3D 10.1002/pro.5560070618
39. C Heussen EB Dowdle 1980 Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substrates Analytical Biochemistry 102 1 196 202 1:CAS:528:DyaL3cXovFOmsg%3D%3D 10.1016/0003-2697(80)90338-3
40. N Hosomi J Lucero JH Heo JA Koziol BR Copeland GJ del Zoppo 2001 Rapid differential endogenous plasminogen activator expression after acute middle cerebral artery occlusion Stroke 32 6 1341 1348 1:CAS:528:DC%2BD3MXltVKru7o%3D
41. MJ Browne AW Tyrrell CG Chapman JE Carey DM Glover FG Grosveld I Dodd JH Robinson 1985 Isolation of a human tissue-type plasminogen-activator genomic DNA clone and its expression in mouse L cells Gene 33 3 279 284 1:CAS:528:DyaL2MXktFOktrg%3D 10.1016/0378-1119(85)90235-5 (Pubitemid 15071251)
42. A Jalanko J Pirhonen G Pohl L Hansson 1990 Production of human tissue-type plasminogen activator in different mammalian cell lines using an Epstein-Barr virus vector Journal of Biotechnology 15 1-2 155 168 1:CAS:528:DyaK3cXltFekt78%3D 10.1016/0168-1656(90)90058-J (Pubitemid 20209189)