Design of an in vivo cleavable disulfide linker in recombinant fusion proteins

BioTechniques

Volumn 49, Issue 1, 2010, Pages 513-518

  Chen, Xiaoying ^a   Bai, Yun ^a   Zaro, Jennica L ^a   Shen, Wei Chiang ^a  

^a UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

Author keywords

Cleavable linker; Disulfide linker; Recombinant fusion protein

Indexed keywords

DISULFIDE; GRANULOCYTE COLONY STIMULATING FACTOR; HYBRID PROTEIN; THROMBIN; TRANSFERRIN; CYCLOPEPTIDE; RECOMBINANT GRANULOCYTE COLONY STIMULATING FACTOR;

ANIMAL EXPERIMENT; ARTICLE; CELL PROLIFERATION; CELL STRAIN HEK293; CHEMICAL BOND; EMBRYO; HUMAN; HUMAN CELL; IN VIVO STUDY; MALE; MOUSE; NONHUMAN; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; PROTEIN SECRETION; WESTERN BLOTTING; ANIMAL; CELL LINE; GENE EXPRESSION; GENETICS; METABOLISM;

ANIMALS; CELL LINE; CELL PROLIFERATION; DISULFIDES; GENE EXPRESSION; GRANULOCYTE COLONY STIMULATING FACTOR, RECOMBINANT; HUMANS; MICE; PEPTIDES, CYCLIC; RECOMBINANT FUSION PROTEINS; THROMBIN; TRANSFERRIN;

EID: 77957961920     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000113450     Document Type: Article

References (23)

1. Uhlen, M., G. Forsberg, T. Moks, M. Hartmanis, and B. Nilsson. 1992. Fusion proteins in biotechnology. Curr. Opin. Biotechnol. 3:363-369.
2. Amet, N., H.F. Lee, and W.C. Shen. 2009. Insertion of the designed helical linker led to increased expression of tf-based fusion proteins. Pharm. Res. 26:523-528.
3. Bai, Y. and W.C. Shen. 2006. Improving the oral efficacy of recombinant granulocyte colony-stimulating factor and transferrin fusion protein by spacer optimization. Pharm. Res. 23:2116-2121.
4. Wriggers, W., S. Chakravarty, and P.A. Jennings. 2005. Control of protein functional dynamics by peptide linkers. Biopolymers 80:736-746. (Pubitemid 43266581)
5. Arai, R., H. Ueda, A. Kitayama, N. Kamiya, and T. Nagamune. 2001. Design of the linkers which effectively separate domains of a bifunctional fusion protein. Protein Eng. 14:529-532. (Pubitemid 32959476)
6. Yao, Z., W. Dai, J. Perry, M.W. Brechbiel, and C. Sung. 2004. Effect of albumin fusion on the biodistribution of interleukin-2. Cancer Immunol. Immunother. 53:404-410.
7. Shen, W.C. 1999. Emerging targeting concepts membrane-associated protein thiol-disulfide interchange activity: a potential target for antiviral and anti-tumor drug design. J. Drug Target. 6:387-389.
8. Shah, D. and W.C. Shen. 1996. Transcellular delivery of an insulin-transferrin conjugate in enterocyte-like Caco-2 cells. J. Pharm. Sci. 85:1306-1311.
9. Widera, A.S., K.-J. Kim, E.D. Crandall, and W.-C. Shen. 2003. Transcytosis of GCSF-transferrin across rat alveolar epithelial cell monolayers. Pharm. Res.20:1231-1238. (Pubitemid 36951848)
10. Widera, A., Y. Bai, and W.C. Shen. 2004. The transepithelial transport of a G-CSF-transferrin conjugate in caco-2 cells and its myelopoietic effect in BDF1 mice. Pharm. Res. 21:278-284.
11. Bai, Y., D.K. Ann, and W.C. Shen. 2005. Recombinant granulocyte colony-stimulating factor-transferrin fusion protein as an oral myelopoietic agent. Proc. Natl. Acad. Sci. USA 102:7292-7296.
12. Aricescu, A.R., W. Lu, and E.Y. Jones. 2006. A time- and cost-efficient system for high-level protein production in mammalian cells. Acta Crystallogr. D Biol. Crystallogr. 62:1243-1250.
13. Hem, A., A.J. Smith, and P. Solberg. 1998. Saphenous vein puncture for blood sampling of the mouse, rat, hamster, gerbil, guinea pig, ferret and mink. Lab. Anim. 32:364-368.
14. Amet, N., W. Wang, and W.C. Shen. 2010. Human growth hormone-transferrin fusion protein for oral delivery in hypophysectomized rats. J. Control. Release 141:177-182.
15. Tamada, T., E. Honjo, Y. Maeda, T. Okamoto, M. Ishibashi, M. Tokunaga, and R. Kuroki. 2006. Homodimeric cross-over structure of the human granulocyte colony-stimulating factor (GCSF) receptor signaling complex. Proc. Natl. Acad. Sci. USA 103:3135-3140.
16. Saito, G., J.A. Swanson, and K.D. Lee. 2003. Drug delivery strategy utilizing conjugation via reversible disulfide linkages: role and site of cellular reducing activities. Adv. Drug Deliv. Rev. 55:199-215.
17. Thorpe, P.E., P.M. Wallace, P.P. Knowles, M.G. Relf, A.N. Brown, G.J. Watson, R.E. Knyba, E.J. Wawrzynczak, and D.C. Blakey. 1987. New coupling agents for the synthesis of immunotoxins containing a hindered disulfide bond with improved stability in vivo. Cancer Res. 47:5924-5931.
18. Letvin, N.L., V.S. Goldmacher, J. Ritz, J.M. Yetz, S.F. Schlossman, and J.M. Lambert. 1986. In vivo administration of lymphocyte-specific monoclonal antibodies in nonhuman primates. In vivo stability of disulfide-linked immunotoxin conjugates. J. Clin. Invest. 77:977-984.
19. Arpicco, S., F. Dosio, P. Brusa, P. Crosasso, and L. Cattel. 1997. New coupling reagents for the preparation of disulfide cross-linked conjugates with increased stability. Bioconjug. Chem. 8:327-337.
20. Xia, C.Q., J. Wang, and W.C. Shen. 2000. Hypoglycemic effect of insulin-transferrin conjugate in streptozotocin-induced diabetic rats. J. Pharmacol. Exp. Ther. 295:594-600.
21. DeLeve, L.D. and N. Kaplowitz. 1991. Glutathione metabolism and its role in hepatotoxicity. Pharmacol. Ther. 52:287-305.
22. Noiva, R. 1999. Protein disulfide isomerase: the multifunctional redox chaperone of the endoplasmic reticulum. Semin. Cell Dev. Biol. 10:481-493.
23. Mandel, R., H.J. Ryser, F. Ghani, M. Wu, and D. Peak. 1993. Inhibition of a reductive function of the plasma membrane by bacitracin and antibodies against protein disulfide-isomerase. Proc. Natl. Acad. Sci. USA 90:4112-4116.