A rapid and sensitive assay for determining human brain levels of farnesyl-(FPP) and geranylgeranylpyrophosphate (GGPP) and transferase activities using UHPLC-MS/MS

Analytical and Bioanalytical Chemistry

Volumn 398, Issue 4, 2010, Pages 1801-1808

  Hooff, Gero P ^a   Patel, Nina ^c   Wood, W Gibson ^b   Müller, Walter E ^a   Eckert, Gunter P ^a   Volmer, Dietrich A ^d  

^a GOETHE UNIVERSITY   (Germany)

^b UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

^c MEDICAL RESEARCH COUNCIL   (United Kingdom)

^d NONE

Author keywords

Brain tissue; Farnesyl pyrophosphate (FPP); Geranylgeranyl pyrophosphate (GGPP); Post translational modification (PTM); Prenylation kinetics; Tandem mass spectrometry; Ultrahigh performance liquid chromatography (UHPLC)

Indexed keywords

BRAIN TISSUE; FARNESYL PYROPHOSPHATES; GERANYLGERANYL PYROPHOSPHATE (GGPP); POST-TRANSLATIONAL MODIFICATIONS; PRENYLATION; TANDEM MASS SPECTROMETRY; ULTRAHIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (UHPLC);

AMINO ACIDS; ASSAYS; CHROMATOGRAPHY; GROWTH KINETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH PRESSURE LIQUID CHROMATOGRAPHY; LIPIDS; LIQUIDS; MASS SPECTROMETRY; TISSUE;

BRAIN;

FARNESYL DIPHOSPHATE; GERANYLGERANYL PYROPHOSPHATE; GERANYLGERANYLTRANSFERASE TYPE I; GERANYLGERANYLTRANSFERASE TYPE-I; ISOPRENOID PHOSPHATE; SESQUITERPENE; SQUALENE SYNTHASE; TRANSFERASE;

ADULT; AGED; ARTICLE; BRAIN; BRAIN LEVEL; CHEMISTRY; ENZYMOLOGY; EVALUATION; FEMALE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; KINETICS; MALE; METABOLISM; METHODOLOGY; MIDDLE AGED; TANDEM MASS SPECTROMETRY;

ADULT; AGED; AGED, 80 AND OVER; ALKYL AND ARYL TRANSFERASES; BRAIN; BRAIN CHEMISTRY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; FARNESYL-DIPHOSPHATE FARNESYLTRANSFERASE; FEMALE; HUMANS; KINETICS; MALE; MIDDLE AGED; POLYISOPRENYL PHOSPHATES; SESQUITERPENES; TANDEM MASS SPECTROMETRY;

EID: 77957848368     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-010-4088-7     Document Type: Article

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