Transfection by electroporation

Current Protocols in Molecular Biology

Volumn , Issue SUPPL. 92, 2010, Pages

  Potter, Huntington ^a   Heller, Richard ^b  

^a UNIVERSITY OF SOUTH FLORIDA   (United States)

^b OLD DOMINION UNIVERSITY   (United States)

Author keywords

DNA vaccine; Gene expression; Gene regulation; Gene therapy; Introduction of DNA into cells; Molecular biology; Transcription and translation

Indexed keywords

PLASMID DNA;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; CELL SUSPENSION; CELL TRANSFORMATION; DNA TRANSFER; ELECTRIC CAPACITANCE; ELECTRIC POTENTIAL; ELECTROPORATION; GENETIC TRANSFECTION; IN VIVO GENE TRANSFER; MAMMAL CELL; METHODOLOGY; MUSCLE TISSUE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTOPLAST; REVIEW; SKIN; TRANSIENT EXPRESSION; ARTICLE; CANCER THERAPY; CONTROLLED STUDY; DNA IMMUNIZATION; EMBRYONIC STEM CELL; FIBROBLAST; GENE THERAPY; IN VIVO STUDY; MOUSE;

ANIMALS; CELLS, CULTURED; ELECTROPORATION; GENE EXPRESSION; GENE THERAPY; MICE; MICE, KNOCKOUT; MICE, TRANSGENIC; PLANTS; PROTOPLASTS; TRANSFECTION; VACCINES, DNA;

MAMMALIA; MUS MUSCULUS;

EID: 77957702514     PISSN: 19343639     EISSN: 19343647     Source Type: Journal    
DOI: 10.1002/0471142727.mb0903s62     Document Type: Review

References (28)

1. Abdulhaqq, S.A., and, Weiner, D.B., 2008. DNA vaccines: Developing new strategies to enhance immune responses. Immunol. Res. 42: 219 - 232.
2. Andre, F., and, Mir, L.M., 2004. DNA electrotransfer: Its principles and an updated review of its therapeutic applications. Gene Ther. 11: S33 - S42.
3. Bodles-Brakhop, A.M., Heller, R., and, Draghia-Akli, R., 2009. Electroporation for the delivery of DNA-based vaccines and immunotherapeutics: Current clinical developments. Mol. Ther. 17: 585 - 592.
4. Bronson, S.K., and, Smithies, O., 1994. Altering mice by homologous recombination using embryonic stem cells. J. Biol. Chem. 269: 27155 - 27158.
5. Chang, D.C., 1989. Cell poration and cell fusion using an oscillating electric field. Biophys. J. 56: 641 - 652.
6. Chu, G., Hayakawa, H., and, Berg, P., 1987. Electroporation for the efficient transfection of mammalian cells with DNA. Nucleic Acids Res. 15: 1311 - 1326.
7. Fromm, M., Taylor, L.P., and, Walbot, V., 1985. Expression of genes transferred into monocot and dicot plant cells by electroporation. Proc. Natl. Acad. Sci. U.S.A. 82: 5824 - 5828.
8. Glasspool-Malone, J., Somiari, S., Drabick, J., and, Malone, R., 2000. Efficient nonviral cutaneous transfection. Mol. Ther. 2: 140 - 146.
9. Gothelf, A., Mir, L.M., and, Gehl, J., 2003. Electrochemotherapy: Results of cancer treatment using enhanced delivery of bleomycin by electroporation. Cancer Treat. Rev. 29: 371 - 387.
10. Heller, L.C., and, Heller, R., 2006. In vivo electroporation for gene therapy. Hum. Gene Ther. 17: 890 - 897.
11. Heller, L.C., Jaroszeski, M.J., Coppola, D., McCrae, A.N., Hickey, J., and, Heller, R., 2006. Optimization of cutaneous electrically mediated plasmid DNA delivery using a novel electrode. Gene Ther. 14: 275 - 280.
12. Heller, R., Jaroszeski, M., Atkin, A., Moradpour, D., Gilbert, R., Wands, J., and, Nicolau, C., 1996. In vivo gene electroinjection and expression in rat liver fed. FEBS Lett. 389: 225 - 228.
13. Hirao, L.A., Wu, L., Khan, A.S., Satishchandran, A., Draghia-Akli, R., and, Weiner, D.B., 2008. Intradermal/subcutaneous immunization by electroporation improves plasmid vaccine delivery and potency in pigs and rhesus macaques. Vaccine 26: 440 - 448.
14. Joyner, A.L., 2000. Gene Targeting. Oxford University Press, Oxford, United Kingdom.
15. Lucas, M.L., and, Heller, R., 2001. Immunomodulation by electrically enhanced delivery of a plasmid encoding IL-12 to murine skeletal muscle. Mol. Ther. 3: 47 - 53.
16. Mir, L.M., Bureau, M.F., Gehl, J., Rangara, R., Rouy, D., Caillaud, J-M., Delaere, P., Branellec, D., Schwartz, B., and, Scherman, D., 1999. High-efficiency gene transfer into skeletal muscle mediated by electric pulses. Proc. Natl. Acad. Sci. U.S.A. 96: 4262 - 4267.
17. Neumann, E., Schaefer-Ridder, M., Wang, Y., and, Hofschneider, P.H., 1982. Gene transfer into mouse lyoma cells by electroporation in high electric fields. EMBO J. 1: 841 - 845.
18. Nishi, T., Yoshizato, K., Yamashiro, S., Takeshima, H., Sato, K., Hamada, K., Kitamura, I., Yoshimura, T., Saya, H., Kuratsu, J., and, Ushio, Y., 1996. High-efficiency in vivo gene transfer using intraarterial plasmid DNA injection following in vivo electroporation. Cancer Res. 56: 1050 - 1055.
19. Ou-Lee, T.M., Turgeon, R., and, Wu, R., 1986. Uptake and expression of a foreign gene linked to either a plant virus or Drosophila promoter in protoplasts of rice, wheat and sorghum. Proc. Natl. Acad. Sci. U.S.A. 83: 6815 - 6819.
20. Potter, H., 1988. Electroporation in biology: Methods, applications, and instrumentation. Anal. Biochem. 174: 361 - 373.
21. Potter, H., Weir, L., and, Leder, P., 1984. Enhancer-dependent expression of human κ immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation. Proc. Natl. Acad. Sci. U.S.A. 81: 7161 - 7165.
22. Rhodes, C.A., Pierce, D.A., Mettler, I.J., Mascarenhas, D., and, Detmar, J.J., 1988. Genetically transformed maize plants from protoplasts. Science 240: 204 - 207.
23. Roos, A.K., Moreno, S., Leder, C., Pavlenko, M., King, A., and, Pisa, P., 2006. Enhancement of cellular immune response to a prostate cancer DNA vaccine by intradermal electroporation. Mol. Ther. 13: 320 - 327.
24. Saunders, J.A., Matthews, B.F., and, Miller, P.D., 1989. Plant gene transfer using electrofusion and electroporation. In Electroporation and Electrofusion in Cell Biology (, E. Neumann, A.E. Sowers, and, C.A. Jordan, eds.) pp. 343 - 354. Plenum, New York.
25. Sheen, J., 1990. Metabolic repression of transcription in higher plants. Plant Cell 2: 1027 - 1038.
26. Titomirov, A.V., Sukharev, S., and, Kistanova, E., 1991. In vivo electroporation and stable transformation of skin cells of newborn mice by plasmid DNA. Biochim. Biophys. Acta 1088: 131 - 134.
27. Trollet, C., Bloquel, C., Scherman, D., and, Bigey, P., 2006. Electrotransfer into skeletal muscle for protein expression. Curr. Gene Ther. 6: 561 - 578.
28. Wong, T.K., and, Neumann, E., 1982. Electric field mediated gene transfer. Biochem. Biophys. Res. Commun. 107: 584 - 587.