Transfection of chicken cerebellar granule neurons used to study glucocorticoid receptor regulation by nuclear receptor 4A (NR4A)

Journal of Neuroscience Methods

Volumn 193, Issue 1, 2010, Pages 39-46

  Strøm, Bjørn O ^a   Aden, Petra ^b   Mathisen, Gro H ^a   Lømo, Jon ^b   Davanger, Svend ^a   Paulsen, Ragnhild E ^a  

^a UNIVERSITY OF OSLO   (Norway)

^b ULLEVÅL UNIVERSITY HOSPITAL   (Norway)

Author keywords

Chicken neuron; Electroporation; Glucocorticoid receptor; NGFI B; NR3C1; NR4A; Transfection

Indexed keywords

DEXAMETHASONE; FORSKOLIN; GLUCOCORTICOID RECEPTOR; NUCLEAR RECEPTOR NUR77; PHORBOL ESTER;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CEREBELLUM; CHICKEN; CONTROLLED STUDY; ELECTROPORATION; GENE CONTROL; GENETIC TRANSFECTION; INTERMETHOD COMPARISON; NERVE CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; REPORTER GENE; TRANSCRIPTION INITIATION;

EID: 77957682424     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2010.08.012     Document Type: Article

References (34)

1. Aden P., Goverud I., Liestøl K., Løberg E.M., Paulsen R.E., Mæhlen J., et al. Low-potency glucocorticoid hydrocortisone has similar neurotoxic effects as high-potency glucocorticoid dexamethasone on neurons in the immature chicken cerebellum. Brain Research 2008, 1236:39-48.
2. Andreason G.L., Evans G.A. Optimization of electroporation for transfection of mammalian cell lines. Analytical Biochemistry 1989, 180:269-275.
3. Cellaxess application note: transfection of dissociated hippocampal Neurons in Culture 2009, Cellectricon, Cellectricon.
4. Cellaxess CX1 system: manual 2009, Cellectricon, Cellectricon.
5. Chen C., Smye S.W., Robinson M.P., Evans J.A. Membrane electroporation theories: a review. Medical and Biological Engineering and Computing 2006, 44:5-14.
6. Ciani E., Paulsen R.E. Avtivation of a reporter gene responsive to NGFI-B in cultured neurons and astrocytes. Journal of Molecular Neuroscience 1995, 6:131-139.
7. Coonrod A., Li F.Q., Horwitz M. On the mechanism of DNA transfection: efficient gene transfer without viruses. Gene Therapy 1997, 4:1313-1321.
8. Dauty E., Verkman A.S. Actin cytoskeleton as the principal determinant of size-dependent DNA mobility in cytoplasm. Journal of Biological Chemistry 2005, 280:7823-7828.
9. Dean D.A., Strong D.D., Zimmer W.E. Nuclear entry of nonviral vectors. Gene Therapy 2005, 12:881-890.
10. Diaz R., Brown R.W., Seckl J.R. Distinct ontogeny of glucocorticoid and mineralocorticoid receptor and 11beta-hydroxysteroid dehydrogenase types I and II mRNAs in the fetal rat brain suggest a complex control of glucocorticoid actions. The Journal of Neuroscience 1998, 18:2570-2580.
11. Drouin J., Maira M., Philips A. Novel mechanism of action for Nur77 and antagonism by glucocorticoids: a convergent mechanism for CRH activation and glucocorticoid repression of POMC gene transcription. The Journal of Steroid Biochemistry and Molecular Biology 1999, 65:59-63.
12. Felgner P.L., Gadek T.R., Holm M., Roman R., Chan H.W., Wenz M., et al. Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure. Proceedings of the National Academy of Sciences of the United States of America 1987, 84:7413-7417.
13. Jacobs C.M., Aden P., Mathisen G.H., Khuong E., Gaarder M., Løberg E.M., et al. Chicken cerebellar granule neurons rapidly develop excitotoxicity in culture. Journal of Neuroscience Methods 2006, 156:129-135.
14. Jacobs C.M., Boldingh K.A., Slagsvold H.H., Thoresen G.H., Paulsen R.E. ERK2 prohibits apoptosis-induced subcellular translocation of orphan nuclear receptor NGFI-B/TR3. Jounal of Biological Chemistry 2004, 279:50097-50101.
15. Kim J.B., Ju J.Y., Kim J.H., Kim T.-Y., Yang B.-H., Lee Y.-S., et al. Dexamethasone inhibits proliferation of adult hippocampal neurogenesis in vivo and in vitro. Brain Research 2004, 1027:1-10.
16. Koumbi D., Clement J.-C., Sideratou Z., Yaouanc J.-J., Loukopoulos D., Kollia P. Factors mediating lipofection potency of a series of cationic phosphonolipids in human cell lines. Biochimica et Biophysica Acta (BBA) - General Subjects 2006, 1760:1151-1159.
17. Martens C., Bilodeau S., Maira M., Gauthier Y., Drouin J. Protein-protein interactions and transcriptional antagonism between the subfamily of NGFI-B/Nur77 orphan nuclear receptors and glucocorticoid receptor. Molecular Endocrinology 2005, 19:885-897.
18. Martin L.J., Tremblay J.J. Glucocorticoids antagonize cAMP-induced Star transcription in Leydig cells through the orphan nuclear receptor NR4A1. Journal of Molecular Endocrinology 2008, 41:165-175.
19. Maruyama K., Tsukada T., Ohkura N., Bandoh S., Hosono T., Yamaguchi K. The NGFI-B subfamily of the nuclear receptor superfamily (review). International Journal of Oncology 1998, 12:1237-1243.
20. Masotti A., Mossa G., Cametti C., Ortaggi G., Bianco A., Grosso N.D., et al. Comparison of different commercially available cationic liposome-DNA lipoplexes: parameters influencing toxicity and transfection efficiency. Colloids and Surfaces B: Biointerfaces 2009, 68:136-144.
21. Maxwell M.A., Muscat G.E. The NR4A subgroup: immediate early response genes with pleiotropic physiological roles. Nuclear Receptor Signaling 2006, 4:e002.
22. McLenachan S., Sarsero J.P., Ioannou P.A. Flow-cytometric analysis of mouse embryonic stem cell lipofection using small and large DNA constructs. Genomics 2007, 89:708-720.
23. Nuclear Receptors Nomenclature Committee A unified nomenclature system for the nuclear receptor superfamily. Cell 1999, 97:161-163.
24. Olofsson J., Levin M., Stromberg A., Weber S.G., Ryttsen F., Orwar O. Generation of focused electric field patterns at dielectric surfaces. Analytical Chemistry 2005, 77:4667-4672.
25. Olofsson J., Levin M., Stromberg A., Weber S.G., Ryttsen F., Orwar O. Scanning electroporation of selected areas of adherent cell cultures. Analytical Chemistry 2007, 79:4410-4418.
26. Paulsen R.E., Granas K., Johnsen H., Rolseth V., Sterri S. Three related brain nuclear receptors, NGFI-B, Nurr1, and NOR-1, as transcriptional activators. Journal of Molecular Neuroscience 1995, 6:249-255.
27. Paulsen R.E., Weaver C.A., Fahrner T.J., Milbrandt J. Domains regulating transcriptional activity of the inducible orphan receptor NGFI-B. Journal of Biological Chemistry 1992, 267:16491-16496.
28. Pavlik A., Buresova M. The neonatal cerebellum: the highest level of glucocorticoid receptors in the brain. Brain Research 1984, 314:13-20.
29. Philips A., Maira M., Mullick A., Chamberland M., Lesage S., Hugo P., et al. Antagonism between Nur77 and glucocorticoid receptor for control of transcription. Molecular and Cellular Biology 1997, 17:5952-5959.
30. Rädler J.O., Koltover I., Salditt T., Safinya C.R. Structure of DNA-cationic liposome complexes: DNA intercalation in multilamellar membranes in distinct interhelical packing regimes. Science 1997, 275:810-814.
31. Smith T.S., Matharu P.J., Sweeney G.E. Cloning and sequencing of a Xenopus homologue of the inducible orphan receptor NGFI-B. Biochim Biophys Acta 1993, 1173:239-242.
32. Tseng W.C., Haselton F.R., Giorgio T.D. Mitosis enhances transgene expression of plasmid delivered by cationic liposomes. Biochimica et Biophysica Acta 1999, 1445:53-64.
33. Washbourne P., McAllister A.K. Techniques for gene transfer into neurons. Current Opinion in Neurobiology 2002, 12:566-573.
34. Zabner J., Fasbender A.J., Moninger T., Poellinger K.A., Welsh M.J. Cellular and molecular barriers to gene transfer by a cationic lipid. The Journal of Biological Chemistry 1995, 270:18997-19007.