Pancreatic islet isolation after gastric bypass in a rat model: Technique and initial results for a promising research tool

Surgery for Obesity and Related Diseases

Volumn 6, Issue 5, 2010, Pages 532-537

  Gatmaitan, Patrick ^a   Huang, Hazel ^a   Talarico, Joseph ^a   Moustarah, Fady ^a   Kashyap, Sangeeta ^a   Kirwan, John P ^a   Schauer, Philip R ^a   Brethauer, Stacy A ^a  

^a LERNER RESEARCH INSTITUTE   (United States)

Author keywords

gut hormones; pancreatic islet stimulation; Roux en Y gastric bypass

Indexed keywords

GASTRIC INHIBITORY POLYPEPTIDE; GLUCAGON LIKE PEPTIDE 1; GLUCOSE; INSULIN; PEPTIDE YY [3-36];

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BODY WEIGHT; CELL ISOLATION; CELL STIMULATION; CONTROLLED STUDY; FEEDING; GLUCOSE BLOOD LEVEL; GLUCOSE HOMEOSTASIS; GLUCOSE METABOLISM; INSULIN BLOOD LEVEL; INSULIN RELEASE; MALE; MORBID OBESITY; NON INSULIN DEPENDENT DIABETES MELLITUS; NONHUMAN; PANCREAS FUNCTION; PANCREAS ISLET BETA CELL; PRIORITY JOURNAL; RAT; ROUX Y ANASTOMOSIS; SPRAGUE DAWLEY RAT; STOMACH BYPASS; TEMPERATURE;

ANALYSIS OF VARIANCE; ANIMALS; BLOOD GLUCOSE; GASTRIC BYPASS; GASTRIC INHIBITORY POLYPEPTIDE; GLUCAGON-LIKE PEPTIDE 1; HYPERGLYCEMIA; INSULIN RESISTANCE; ISLETS OF LANGERHANS; MALE; MODELS, ANIMAL; OBESITY; PEPTIDE YY; RANDOM ALLOCATION; RATS; RATS, SPRAGUE-DAWLEY;

EID: 77957553516     PISSN: 15507289     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.soard.2010.05.018     Document Type: Article

References (23)

1. H. Buchwald, Y. Avidor, and E. Braunwald Bariatric surgery: a systematic review and meta-analysis JAMA 292 2004 1724 1737
2. J.B. Dixon, P.E. O'Brien, and J. Playfair Adjustable gastric banding and conventional therapy for type 2 diabetes: a randomized controlled trial JAMA 299 2008 316 323
3. W.J. Pories, M.S. Swanson, and K.G. MacDonald Who would have thought it? An operation proves to be the most effective therapy for adult-onset diabetes mellitus Ann Surg 222 1995 339 352
4. P.R. Schauer, B. Burguera, and S. Ikramuddin Effect of laparoscopic Roux-en Y gastric bypass on type 2 diabetes mellitus Ann Surg 238 2003 467 485
5. K. Wickremesekera, G. Miller, and T.D. Naotunne Loss of insulin resistance after Roux-en-Y gastric bypass surgery: a time course study Obes Surg 15 2005 474 481
6. 2: a report of 2 cases Surg Obes Relat Dis 3 2007 195 197
7. A. Torquati, R. Lutfi, N. Abumrad, and W.O. Richards Is Roux-en-Y gastric bypass surgery the most effective treatment for type 2 diabetes mellitus in morbidly obese patients? J Gastrointest Surg 9 2005 1112 1118
8. D. Pacheco, D.A. de Luis, and A. Romero The effects of duodenal-jejunal exclusion on hormonal regulation of glucose metabolism in Goto-Kakizaki rats Am J Surg 194 2007 221 224
9. F. Rubino, A. Forgione, and D.E. Cummings The mechanism of diabetes control after gastrointestinal bypass surgery reveals a role of the proximal small intestine in the pathophysiology of type 2 diabetes Ann Surg 244 2006 741 749
10. F. Rubino, and J. Marescaux Effect of duodenal-jejunal exclusion in a non-obese animal model of type 2 diabetes: a new perspective for an old disease Ann Surg 239 2004 1 11 (Pubitemid 38176365)
11. J. Korner, M. Bessler, and W. Inabnet Exaggerated glucagon-like peptide-1 and blunted glucose-dependent insulinotropic peptide secretion are associated with Roux-en-Y gastric bypass but not adjustable gastric banding Surg Obes Relat Dis 3 2007 597 601
12. C.W. le Roux, S.J. Aylwin, and R.L. Batterham Gut hormone profiles following bariatric surgery favor an anorectic state, facilitate weight loss, and improve metabolic parameters Ann Surg 243 2006 108 114
13. C.W. le Roux, R. Welbourn, and M. Werling Gut hormones as mediators of appetite and weight loss after Roux-en-Y gastric bypass Ann Surg 246 2007 780 785
14. A.D. Strader, T.P. Vahl, and R.J. Jandacek Weight loss through ileal transposition is accompanied by increased ileal hormone secretion and synthesis in rats Am J Physiol Endocrinol Metab 288 2005 E447 E453
15. L.L. Baggio, and D.J. Drucker Biology of incretins: GLP-1 and GIP Gastroenterology 132 2007 2131 2157
16. K. Meirelles, T. Ahmed, and D.M. Culnan Mechanisms of glucose homeostasis after Roux-en-Y gastric bypass surgery in the obese, insulin-resistant Zucker rat Ann Surg 249 2009 277 285
17. S.R. Kashyap, S. Daud, and K.R. Kelly Acute effects of gastric bypass versus gastric restrictive surgery on beta-cell function and insulinotropic hormones in severely obese patients with type 2 diabetes Int J Obes (Lond) Epub 2009 Dec 22
18. R.H. Clements, Q.H. Gonzalez, and C.I. Long Hormonal changes after Roux-en-Y gastric bypass for morbid obesity and the control of type-II diabetes mellitus Am Surg 70 2004 1 5
19. P.R. Flatt Effective surgical treatment of obesity may be mediated by ablation of the lipogenic gut hormone gastric inhibitory polypeptide (GIP): evidence and clinical opportunity for development of new obesity-diabetes drugs? Diab Vasc Dis Res 4 2007 151 153
20. B. Laferrere, S. Heshka, and K. Wang Incretin levels and effect are markedly enhanced 1 month after Roux-en-Y gastric bypass surgery in obese patients with type 2 diabetes Diabetes Care 30 2007 1709 1716
21. M.M. Meguid, E.J. Ramos, and S. Suzuki A surgical rat model of human Roux-en-Y gastric bypass J Gastrointest Surg 8 2004 621 630
22. P.E. Lacy, and M. Kostianovsky Method for the isolation of intact islets of Langerhans from the rat pancreas Diabetes 16 1967 35 39
23. B.P. Cummings, E.K. Digitale, and K.L. Stanhope Development and characterization of a novel rat model of type 2 diabetes mellitus: the UC Davis type 2 diabetes mellitus UCD-T2DM rat Am J Physiol Regul Integr Comp Physiol 295 2008 R1782 R1793