Deletion of the elongation factor 4 gene (lepA) in Streptomyces coelicolor enhances the production of the calcium-dependent antibiotic

FEMS Microbiology Letters

Volumn 311, Issue 2, 2010, Pages 147-151

  Badu Nkansah, Akosua ^a   Sello, Jason K ^a  

^a BROWN UNIVERSITY   (United States)

Author keywords

antibiotic production; elongation factor 4; lepA; ribosome; Streptomyces; translational GTPase

Indexed keywords

ANTIBIOTIC AGENT; CALCIUM DEPENDENT ANTIBIOTIC; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; CONTROLLED STUDY; GENE DELETION; LEPA BACTERIAL GENE; NONHUMAN; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN STRUCTURE; RIBOSOME; STREPTOMYCES COELICOLOR;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; CALCIUM; GENE DELETION; GENE EXPRESSION REGULATION, BACTERIAL; PEPTIDE ELONGATION FACTORS; STREPTOMYCES COELICOLOR;

STREPTOMYCES; STREPTOMYCES COELICOLOR;

EID: 77957311620     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2010.02083.x     Document Type: Article

References (29)

1. Bauerschmitt H, Funes S Herrmann JM (2008) The membrane-bound GTPase Guf1 promotes mitochondrial protein synthesis under suboptimal conditions. J Biol Chem 283 : 17139 17146.
2. Bentley SD, Chater KF, Cerdeño-Tarraga AM et al 2002) Complete genome sequence of the model actinomycete Streptomyces coelicolor. Nature 417 : 141 147.
3. Bijlsma JJ, Lie-A-Ling M, Nootenboom IC, Vandenbroucke-Grauls CM Kusters JG (2000) Identification of loci essential for the growth of Helicobacter pylori under acidic conditions. J Infect Dis 182 : 1566 1569.
4. Caldon CE, Yoong P March PE (2001) Evolution of a molecular switch: universal bacterial GTPases regulate ribosome function. Mol Microbiol 41 : 289 297.
5. Chong PP, Podmore SM, Kieser HM, Redenbach M, Turgay K, Marahiel M, Hopwood DA Smith CP (1998) Physical identification of a chromosomal locus encoding biosynthetic genes for the lipopeptide calcium-dependent antibiotic (CDA) of Streptomyces coelicolor A3(2). Microbiology 144 : 193 199.
6. Colca JR, McDonald WG, Waldon DJ et al 2003) Cross-linking in the living cell locates the site of action of oxazolidinone antibiotics. J Biol Chem 278 : 21972 21979.
7. Dibb NJ Wolfe PB (1986) Lep operon proximal gene is not required for growth or secretion by Escherichia coli. J Bacteriol 166 : 83 87.
8. Evans RN, Blaha G, Bailey S Steitz TA (2008) The structure of LepA, the ribosomal backtranslocase. P Natl Acad Sci USA 105 : 4673 4678.
9. Gregory MA, Till R Smith MCM (2003) Integration site for Streptomyces phage ΦBT1 and development of site-specific integrating vectors. J. Bacteriol 185 : 5320 5323.
10. Gust B, Challis GL, Fowler K, Kieser T Chater KF (2003) PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin. P Natl Acad Sci USA 100 : 1541 1546.
11. Hesketh AR, Chandra G, Shaw AD, Rowland JJ, Kell DB, Bibb MJ Chater KF (2002) Primary and secondary metabolism, and post-translational protein modifications, as portrayed by proteomic analysis of Streptomyces coelicolor. Mol Microbiol 46 : 917 932.
12. Hojati Z, Milne C, Harvey B et al 2002) Structure, biosynthetic origin, and engineered biosynthesis of calcium-dependent antibiotics from Streptomyces coelicolor. Chem Biol 11 : 1175 1187.
13. Hopwood DA (1979) Genetics of antibiotic production by Actinomycetes. J Nat Prod 42 : 596 602.
14. Hopwood DA Wright HM (1983) CDA is a new chromosomally-determined antibiotic from Streptomyces coelicolor A3(2). Microbiology 129 : 3575 3579.
15. Huang J, Lih CJ, Pan KH Cohen SN (2001) Global analysis of growth phase responsive gene expression and regulation of antibiotic biosynthetic pathways in Streptomyces coelicolor using DNA microarrays. Gene Dev 23 : 3183 3192.
16. Jones GH, Paget MS, Chamberlin L Buttner MJ (1997) Sigma-E is required for the production of the antibiotic actinomycin in Streptomyces antibioticus. Mol Microbiol 23 : 169 178.
17. Kieser T, Bibb MJ, Buttner MJ, Chater KF Hopwood DA (2000) Practical Streptomyces Genetics. John Innes Foundation, Norwich, UK.
18. Lipmann F, Gevers W, Kleinkauf H Roskoski R Jr. (1971) Polypeptide synthesis on protein templates: the enzymatic synthesis of gramicidin S and tyrocidine. Adv Enzymol RAMB 35 : 1 34.
19. March PE Inouye M (1985) GTP-binding membrane protein of Escherichia coli with sequence homology to initiation factor 2 and elongation factors Tu and G. P Natl Acad Sci USA 82 : 7500 7504.
20. Margus T, Remm M Tenson T (2007) Phylogenetic distribution of translation GTPases in bacteria. BMC Genomics 8 : 15.
21. Ochi K, Okamoto S, Tozawa Y, Inaoka T, Hosaka T, Xu J Kurosawa K (2004) Ribosome engineering and secondary metabolite production. Adv Appl Microbiol 56 : 155 184.
22. Qin Y, Polacek N, Vesper O, Staub E, Einfeldt E, Wilson DN Nierhaus KH (2006) The highly conserved LepA is a ribosomal elongation factor that backtranslocates the ribosome. Cell 127 : 721 733.
23. Sambrook J Russell DW (2001) Molecular Cloning: A Laboratory Manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
24. Schwarzer D, Finking R Marahiel MA (2003) Nonribosomal peptides: from genes to products. Nat Prod Rep 3 : 275 287.
25. Shoji S, Janssen BD, Hayes CS Frederick K (2010) Translation factor LepA contributes to tellurite resistance in Escherichia coli but plays no apparent role in the fidelity of protein synthesis. Biochimie 92 : 157 163.
26. Steitz TA (2008) A structural understanding of the dynamic ribosome machine. Nat Rev Mol Cell Bio 9 : 242 253.
27. Wang G, Hosaka T Ochi K (2008) Dramatic activation of antibiotic production in Streptomyces coelicolor by cumulative drug resistance mutations. Appl Environ Microb 74 : 2834 2840.
28. Vecchione JJ Sello JK (2008) Characterization of an inducible, antibiotic-resistant aminoacyl-tRNA synthetase gene in Streptomyces coelicolor. J Bacteriol 190 : 6253 6257.
29. Vinci VA Byng G (1999) Strain improvement by nonrecombinant methods. Manual of Industrial Microbiology and Biotechnology (Demain A Davies J, eds), pp. 103 113. ASM Press, Washington, DC.