The unusual extended signal peptide region is not required for secretion and function of an Escherichia coli autotransporter

FEMS Microbiology Letters

Volumn 311, Issue 2, 2010, Pages 133-139

  Leyton, Denisse L ^a   De Luna, Maria Das Graças ^b   Sevastsyanovich, Yanina R ^a   Tveen Jensen, Karina ^a   Browning, Douglas F ^a   Scott Tucker, Anthony ^a   Henderson, Ian R ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^b UNIVERSIDADE DO ESTADO DO RIO DE JANEIRO   (Brazil)

Author keywords

autotransporter; Escherichia coli; extended signal peptide region (ESPR); plasmid encoded toxin (Pet); secretion

Indexed keywords

EXTENDED SIGNAL PEPTIDE REGION; SERINE PROTEINASE; SERINE PROTEINASE PET; SIGNAL PEPTIDE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN PROCESSING; PROTEIN SECRETION;

AMINO ACID SEQUENCE; BACTERIAL TOXINS; ENTEROTOXINS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MOLECULAR SEQUENCE DATA; PROTEIN FOLDING; PROTEIN SORTING SIGNALS; PROTEIN STRUCTURE, TERTIARY; PROTEIN TRANSPORT; SEQUENCE ALIGNMENT; SEQUENCE DELETION; SERINE ENDOPEPTIDASES;

BACTERIA (MICROORGANISMS); ENTEROBACTERIACEAE; ESCHERICHIA COLI;

EID: 77957309698     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2010.02081.x     Document Type: Article

References (20)

1. Chevalier N, Moser M, Koch H G, Schimz K L, Willery E, Locht C, Jacob-Dubuisson F Müller M (2004) Membrane targeting of a bacterial virulence factor harbouring an extended signal peptide. J Mol Microb Biotech 8 : 7 18.
2. Desvaux M, Cooper LM, Filenko NA, Scott-Tucker A, Turner SM, Cole JA Henderson IR (2006) The unusual extended signal peptide region of the type V secretion system is phylogenetically restricted. FEMS Microbiol Lett 264 : 22 30.
3. Desvaux M, Scott-Tucker A, Turner SM, Cooper LM, Huber D, Nataro JP Henderson IR (2007) A conserved extended signal peptide region directs posttranslational protein translocation via a novel mechanism. Microbiology 153 : 59 70.
4. Eslava C, Navarro-Garcia F, Czeczulin JR, Henderson IR, Cravioto A Nataro JP (1998) Pet, an autotransporter enterotoxin from enteroaggregative Escherichia coli. Infect Immun 66 : 3155 3163.
5. Guyer DM, Henderson IR, Nataro JP Mobley HLT (2000) Identification of Sat, an autotransporter toxin produced by uropathogenic Escherichia coli. Mol Microbiol 38 : 53 66.
6. Henderson IR, Navarro-Garcia F Nataro JP (1998) The great escape: structure and function of the autotransporter proteins. Trends Microbiol 6 : 370 378.
7. Henderson IR, Navarro-Garcia F, Desvaux M, Fernandez RC Ala'Aldeen D (2004) Type V protein secretion pathway: the autotransporter story. Microbiol Mol Biol R 68 : 692 744.
8. Hiss JA Schneider G (2009) Domain organization of long autotransporter signal sequences. Bioinfor Biol Insights 2009 : 189 204.
9. Jacob-Dubuisson F, Fernandez R Coutte L (2004) Protein secretion through autotransporter and two-partner pathways. Biochim Biophys Acta 1694 : 235 257. (Pubitemid 39535072)
10. Jong WSP Luirink J (2008) The conserved extension of the Hbp autotransporter signal peptide does not determine targeting pathway specificity. Biochem Bioph Res Co 368 : 522 527.
11. Kumamoto CA Beckwith J (1985) Evidence for specificity at an early step in protein export in Escherichia coli. J Bacteriol 163 : 267 274.
12. Lambert-Buisine C, Willery E, Locht C Jacob-Dubuisson F (1998) N-terminal characterization of the Bordetella pertussis filamentous haemagglutinin. Mol Microbiol 28 : 1283 1293.
13. Leyton DL, Sloan J, Hill RE, Doughty S Hartland EL (2003) Transfer region of pO113 from Enterohemorrhagic Escherichia coli: similarity with R64 and identification of a novel plasmid-encoded autotransporter, EpeA. Infect Immun 71 : 6307 6319.
14. Nielsen H, Engelbrecht J, Brunak S von Heijne G (1997) Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng 10 : 1 6.
15. Peterson JH, Woolhead CA Bernstein HD (2003) Basic amino acids in a distinct subset of signal peptides promote interaction with the signal recognition particle. J Biol Chem 278 : 46155 46162.
16. Peterson JH, Szabady RL Bernstein HD (2006) An unusual signal peptide extension inhibits the binding of bacterial presecretory proteins to the signal recognition particle, trigger factor, and the SecYEG complex. J Biol Chem 281 : 9038 9048.
17. Sambrook J, Fritsch E F Maniatis T (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Press, Cold Spring Harbor, NY.
18. Schierle C F, Berkmen M, Huber D, Kumamoto C, Boyd D Beckwith J (2003) The DsbA signal sequence directs efficient, cotranslational export of passenger proteins to the Escherichia coli periplasm via the signal recognition particle pathway. J Bacteriol 185 : 5706 5713.
19. Sijbrandi R, Urbanus M L, ten Hagen-Jongman C M, Bernstein H D, Oudega B, Otto B R Luirink J (2003) Signal recognition particle (SRP)-mediated targeting and Sec-dependent translocation of an extracellular Escherichia coli protein. J Biol Chem 278 : 4654 4659.
20. Szabady RL, Peterson JH, Skillman KM Bernstein HD (2005) An unusual signal peptide facilitates late steps in the biogenesis of a bacterial autotransporter. P Natl Acad Sci USA 102 : 221 226.