Comparative kinetics of cofactor association and dissociation for the human and trypanosomal S-adenosylhomocysteine hydrolases. 3. Role of lysyl and tyrosyl residues of the C-terminal extension

Biochemistry

Volumn 49, Issue 38, 2010, Pages 8434-8441

  Cai, Sumin ^a   Fang, Jianwen ^a   Li, Qing Shan ^a   Borchardt, Ronald T ^a   Kuczera, Krzysztof ^a   Middaugh, C Russell ^a   Schowen, Richard L ^a  

^a UNIVERSITY OF KANSAS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; DISSOCIATION; ENZYMES; FREE ENERGY; MUTAGENESIS; PHOSPHATASES;

ASSOCIATION AND DISSOCIATION KINETICS; BINDING FREE ENERGY; C-TERMINAL EXTENSIONS; COFACTOR AFFINITY; COFACTOR BINDING; COFACTORS; COMPARATIVE STUDIES; ENERGY DECOMPOSITION; EQUILIBRIUM DISSOCIATION CONSTANT; EXPERIMENTAL STUDIES; FREE ENERGY SIMULATIONS; HOMOLOGOUS PROTEINS; HYDROLASES; LYSINE RESIDUES; NADH BINDING; ORTHOLOGOUS PROTEINS; S-ADENOSYLHOMOCYSTEINE; TETRAMERIC STRUCTURES; TYROSINE RESIDUES; WILD TYPES; X-RAY STRUCTURE;

BINDING ENERGY;

ADENOSYLHOMOCYSTEINASE; LYSINE; NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; TETRAMER; TYROSINE; S ADENOSYLHOMOCYSTEINE;

ARTICLE; BINDING AFFINITY; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; CRYSTAL STRUCTURE; DISSOCIATION; DISSOCIATION CONSTANT; ENZYME STRUCTURE; EQUILIBRIUM CONSTANT; KINETICS; MOLECULAR MECHANICS; MUTAGENESIS; MUTANT; PREDICTION; PRIORITY JOURNAL; SIMULATION; WILD TYPE; X RAY CRYSTALLOGRAPHY; CHEMISTRY; COMPARATIVE STUDY; HUMAN; METABOLISM; TRYPANOSOMA; X RAY;

ADENOSYLHOMOCYSTEINASE; BINDING SITES; HUMANS; KINETICS; NAD; S-ADENOSYLHOMOCYSTEINE; TRYPANOSOMA; X-RAYS;

EID: 77957057042     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi1007595     Document Type: Article

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