Increased proliferation and analysis of differential gene expression in human Wharton's jelly-derived mesenchymal stromal cells under hypoxia

International Journal of Biological Sciences

Volumn 6, Issue 5, 2010, Pages 499-512

  Nekanti, Usha ^a   Dastidar, Sumitava ^c   Venugopal, Parvathy ^a   Totey, Satish ^b   Ta, Malancha ^c  

^a MANIPAL HOSPITAL   (India)

^b Stempeutics Research Malaysia Sdn Bhd   (Malaysia)

^c Manipal Academy of Higher Education   (India)

Author keywords

Cell proliferation; Hypoxia; Mesenchymal stem cells (MSCs); Transcription; Transformation markers; Wharton's jelly

Indexed keywords

EID: 77956961377     PISSN: 14492288     EISSN: None     Source Type: Journal    
DOI: 10.7150/ijbs.6.499     Document Type: Article

References (40)

1. Pittenger MF, Mackay AM, Beck SM, et al. Multilineage potential of adult human mesenchymal stem cells. Science. 1999; 284: 143-147.
2. Le Blanc K. Immunomodulatory effects of fetal and adult mesenchymal stem cells. Cytotherapy. 2003; 5: 485-489.
3. Troyer DL, Weiss ML. Wharton's jelly-derived cells are a primitive stromal cell population. Stem cells. 2008; 26: 591-599.
4. Ezashi T, Das P, Roberts RM. Low O2 tensions and the prevention of differentiation of hES cells. Proc Natl Acad Sci U S A. 2005;102: 4783-4788.
5. Gassmann M, Fandrey J, Bichet S, et al. Oxygen supply and oxygen-dependent gene expression in differentiating embryonic stem cells. Proc Natl Acad Sci U S A. 1996; 93: 2867-2872.
6. Ma T, Grayson WL, Fröhlich M, et al. Hypoxia and stem cell-based engineering of mesenchymal tissues. Biotechnol Prog. 2009; 25(1): 32-42.
7. Artavanis-Tsakonas S, Rand MD, Lake RJ. Notch signaling: cell fate control and signal integration in development. Science. 1999; 284: 770-776.
8. Wang GL, Semenza GL. Purification and characterization of hypoxia-inducible factor 1. J Biol Chem. 1995; 270: 1230-1237.
9. Kallio PJ, Pongratz I, Gradin K, et al. Activation of hypoxia-inducible factor 1alpha: posttranscriptional regulation and conformational change by recruitment of the Arnt transcription factor. Proc Natl Acad Sci U S A. 1997; 94: 5667-5672.
10. Gustafsson MV, Zheng X, Pereira T, et al. Hypoxia requires notch signaling to maintain the undifferentiated cell state. Dev Cell. 2005; 9: 617-628
11. Harris AL. Hypoxia--a key regulatory factor in tumour growth. Nat Rev Cancer. 2002; 2: 38-47.
12. Kivisaari J. Oxygen and carbon dioxide tensions in healing tissue. Acta Chir Scand. 1975;141: 693-696.
13. Stevens CR, Williams RB, Farrell AJ, et al. Hypoxia and inflammatory synovitis: observations and speculation. Ann Rheum Dis. 1991; 50: 124-132.
14. Jürgensen JS, Rosenberger C, Wiesener MS, et al. Persistent induction of HIF-1alpha and -2alpha in cardiomyocytes and stromal cells of ischemic myocardium. Faseb J. 2004;18: 1415-1417.
15. Tse HF, Kwong YL, Chan JK, et al. Angiogenesis in ischaemic myocardium by intramyocardial autologous bone marrow mononuclear cell implantation. Lancet. 2003; 361: 47-49.
16. Tateishi-Yuyama E, Matsubara E, Murohara T, et al. Therapeutic angiogenesis for patients with limb ischaemia by autologous transplantation of bone-marrow cells: a pilot study and a randomised controlled trial. Lancet. 2002; 360: 427-435.
17. Studer L, Csete M, Lee SH, et al. Enhanced proliferation, survival, and dopaminergic differentiation of CNS precursors in lowered oxygen. J Neurosci. 2000; 20: 7377-7383.
18. Morrison SJ, Csete M, Groves AK, et al. Culture in reduced levels of oxygen promotes clonogenic sympathoadrenal differentiation by isolated neural crest stem cells. J Neurosci. 2000; 20: 7370-7376.
19. Malladi P, Xu Y, Chiou M, et al. Effect of reduced oxygen tension on chondrogenesis and osteogenesis in adipose-derived mesenchymal cells. Am J Physiol Cell Physiol. 2006; 290: C1139-1146.
20. Lennon DP, Edmison JM, Caplan AI. Cultivation of rat marrow-derived mesenchymal stem cells in reduced oxygen tension: effects on in vitro and in vivo osteochondrogenesis. J Cell Physiol. 2001;187: 345-355.
21. Dachs GU, Patterson AV, Firth JD, et al. Targeting gene expression to hypoxic tumor cells. Nat Med. 1997; 3: 515-520.
22. Fischer S, Wiesnet M, Marti HH, et al. Simultaneous activation of several second messengers in hypoxia-induced hyperpermeability of brain derived endothelial cells. J Cell Physiol. 2004;198: 359-369.
23. Nekanti U, Rao VB, Bahirvani AG, et al. Long-term Expansion and Pluripotent Marker Array Analysis of Wharton's Jelly-Derived Mesenchymal Stem Cells. Stem Cells Dev. 2010; 19: 117-130.
24. Wagner W, Horn P, Castoldi M, et al. Replicative senescence of mesenchymal stem, cells: a continuous and organized process. PLoS One. 2008; 3: 2213
25. Prasad SM, Czepiel M, Cetinkaya C, et al. Continuous hypoxic culturing maintains activation of Notch and allows long-term propagation of human embryonic stem cells without spontaneous differentiation. Cell Prolif. 2009; 42: 63-74.
26. Rubio D, Garcia-Castro J, Martín MC, et al. Spontaneous human adult stem cell transformation. Cancer Res. 2005; 65: 3035-3039.
27. Bongso A, Fong CY, Gauthaman K. Taking stem cells to the clinic: Major challenges. J Cell Biochem. 2008;105: 1352-1360.
28. Karahuseyinoglu S, Cinar O, Kilic E, et al. Biology of stem cells in human umbilical cord stroma: in situ and in vitro surveys. Stem Cells. 2007; 25: 319-331.
29. Lund RD, Wang S, Lu B, et al. Cells isolated from umbilical cord tissue rescue photoreceptors and visual functions in a rodent model of retinal disease. Stem Cells. 2007; 25: 602-611.
30. Ren H, Cao Y, Zhao Q, et al. Proliferation and differentiation of bone marrow stromal cells under hypoxic conditions. Biochem Biophys Res Commun. 2006; 347: 12-21
31. Grayson WL, Zhao F, Bunnell B, et al. Hypoxia enhances proliferation and tissue formation of human mesenchymal stem cells. Biochem Biophys Res Commun. 2007; 358: 948-953
32. Ohnishi S, Yasuda T, Kitamura S, et al. Effect of hypoxia on gene expression of bone marrow-derived mesenchymal stem cells and mononuclear cells. Stem Cells. 2007; 25: 1166-1177.
33. Ratcliffe PJ. HIF-1 and HIF-2: Working alone or together in hypoxia? J Clin Invest. 2007; 117: 862-865.
34. Rankin EB, Giaccia AJ. The role of hypoxia-inducible factors in tumorigenesis. Cell Death Differ. 2008; 15: 678-685.
35. Zhong H, De Marzo AM, Laughner E, et al. Overexpression of hypoxia-inducible factor 1alpha in common human cancers and their metastases. Cancer Res. 1999; 59: 5830-5835.
36. Potier E, Ferreira E, Andriamanalijaona R, et al. Hypoxia affects mesenchymal stromal cell osteogenic differentiation and angiogenic factor expression. Bone. 2007; 40: 1078-1087.
37. Rosová I, Dao M, Capoccia B, et al. Hypoxic preconditioning results in increased motility and improved therapeutic potential of human mesenchymal stem cells. Stem Cells. 2008;26: 2173-2182.
38. Nagano M, Kimura K, Yamashita T, et al. Hypoxia responsive mesenchymal stem cells derived from human umbilical cord blood are effective for bone repair. Stem Cells Dev. 2010; [Epub ahead of print].
39. Yin F, Herring BP. GATA-6 can act as a positive or negative regulator of smooth muscle-specific gene expression. J Biol Chem. 2004; 280: 4745-4752.
40. Iwaki T, Yamamoto K, Matsuura T, et al. Alteration of integrins under hypoxic stress in early placenta and choriocarcinoma cell line BeWo. Gynecol Obstet Invest. 2004; 57: 196-203.