His-tagged protein purification by metal-chelate affinity extraction with Nickel-chelate reverse micelles

Biotechnology Progress

Volumn 26, Issue 4, 2010, Pages 1088-1094

  Dong, Xiao Yan ^a   Feng, Xu Dong ^a   Sun, Yan ^a  

^a TIANJIN UNIVERSITY   (China)

Author keywords

Affinity extraction; Enhanced green fluorescent protein; Metal chelate binding; Protein purification; Reverse micelle

Indexed keywords

AFFINITY BINDING; AFFINITY EXTRACTION; BACK EXTRACTION; DI(2-ETHYLHEXYL)PHOSPHORIC ACID; ENHANCED GREEN FLUORESCENT PROTEIN; FLUORESCENCE SPECTRUM ANALYSIS; HIGH SPECIFICITY; HIS-TAGGED PROTEINS; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; IMPURITIES IN; NATIVE PROTEINS; NONIONIC; ORGANIC PHASE; PROTEIN PURIFICATION; REVERSE MICELLAR SYSTEM; REVERSE MICELLES; SEPARATION PROCESS; SPAN 80; TRITON-X; TWO STAGE;

AFFINITY CHROMATOGRAPHY; CHELATION; ESCHERICHIA COLI; FLUORESCENCE; METAL IONS; METAL RECOVERY; MICELLES; OLIGOMERS; PHOSPHATASES; PHOSPHORIC ACID; PROTEINS; PURIFICATION; SPECTRUM ANALYSIS; SPECTRUM ANALYZERS; TRANSITION METAL COMPOUNDS; TRANSITION METALS;

EXTRACTION;

CHELATING AGENT; ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; HISTIDINE; NICKEL; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; CHEMISTRY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MICELLE;

CHELATING AGENTS; CHROMATOGRAPHY, AFFINITY; GREEN FLUORESCENT PROTEINS; HISTIDINE; MICELLES; NICKEL; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 77956953191     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.428     Document Type: Article

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