Protein extraction using non-ionic reverse micelles of Span 60

Biochemical Engineering Journal

Volumn 2, Issue 2, 1998, Pages 113-119

  Naoe, Kazumitsu ^a   Ura, Osamu ^a   Hattori, Mitsunari ^a   Kawagoe, Mikio ^a   Imai, Masanao ^b,c  

^a NARA NATIONAL COLLEGE OF TECHNOLOGY   (Japan)

^b NIHON UNIVERSITY   (Japan)

^c TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

Author keywords

Extraction; Non ionic surfactant; Protein; Reverse micelle; Span 60

Indexed keywords

EID: 0000460862     PISSN: 1369703X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1369-703X(98)00023-0     Document Type: Article

References (24)

1. V. Brandani, G.D. Giacomo, L. Spera, Recovery of α-amylase extracted by reverse micelles, Process Biochem. 31 (1996) 125-128.
2. Q.-L. Chang, J.-Y. Chen, Liquid-liquid reversed micellar extraction for isolating enzymes, Studies on the purification of α-amylase, Process Biochem. 31 (1996) 371-375.
3. K. Naoe, M. Tamai, M. Kawagoe, M. Imai, M. Shimizu, Minimal AOT concentration of binary protein component in reverse micellar extraction, Trans. IChemE. Part C 75 (1997) 143-147.
4. R. Kuboi, Y. Mori, I. Komasawa, Reverse micelle size distribution and mechanism of protein solubilization into reverse micelles (in Japanese), Kagaku Kogaku Ronbunshu 16 (1990) 763-771.
5. K.E. Göklen, T.A. Hatton, Liquid-liquid extraction of low molecular-weight proteins by selective solubilization in reversed micelles, Sep. Sci. Technol. 22 (1987) 831-841.
6. G.J. Lye, J.A. Asenjo, D.L. Pyle, Reverse micellar mass-transfer processes, spray column extraction of lysozyme, AIChE J. 42 (1996) 713-726.
7. S. Ichikawa, M. Imai, M. Shimizu, Solubilizing water involved in protein extraction using reversed micelles, Biotechnol. Bioeng. 39 (1992) 20-26.
8. R. Kuboi, Y. Mori, I. Komasawa, Denaturation and deactivation of porcine pancreas lipase in reverse micelle extraction (in Japanese), Kagaku Kogaku Ronbunshu 16 (1990) 1060-1066.
9. K. Naoe, M. Imai, M. Shimizu, Optimal amphiphile concentration for lysozyme extraction using reverse micelles, Trans. IChemE, Part C 74 (1996) 163-170.
10. Y. Yamada, R. Kuboi, I. Komasawa, Extraction of enzymes and their activities in AOT reverse micellar systems modified with nonionic surfactants, J. Chem. Eng. Jpn. 27 (1994) 404-409.
11. C.F. Komives, E. Lilley, A.J. Russell, Biodegradation of pesticides in nonionic water-in-oil microemulsions of Tween 85, Relationship between micelle structure and activity, Biotechnol. Bioeng. 43 (1994) 946-959.
12. G.A. Ayala, S. Kamat, E.J. Bechman, A.J. Russell, Protein extraction and activity in reverse micelles of a nonionic detergent, Biotechnol. Bioeng. 39 (1992) 806-814.
13. M. Vasudevan, K. Tahan, J.M. Wiencek, Surfactant structure effects in protein separations using nonionic microemulsions, Biotechnol. Bioeng. 46 (1995) 99-108.
14. F. Kamiwatari, Physical associative modification of protein by amphiphilic molecules (in Japanese), BS thesis, Tokyo University of Agriculture and Technology, Japan, 1990.
15. M. Vasudevan, J.M. Wiencek, Mechanism of the extraction of proteins into Tween 85 nonionic microemulsions, Ind. Eng. Chem. Res. 35 (1996) 1085-1089.
16. D. Balzer, Cloud point phenomena in the phase behavior of alkyl polyglucosides in water, Langmuir 9 (1993) 3375-3385.
17. M.P. Pileni, T. Zemb, C. Petit, Solubilization by reverse micelles: Solute localization and structure perturbation, Chem. Phys. Lett. 118 (1985) 414-420.
18. C.F. Komives, D.E. Osborme, A.J. Russell, Characterization of a nonionic surfactant reverse micellar system for enzyme catalysis, J. Phys. Chem. 98 (1994) 369-376.
19. M. Imai, T. Natsume, K. Naoe, M. Shimizu, S. Ichikawa, S. Furusaki, Hydrophilic surroundings requisite for the solubilization of proteins related with their hydrophobicity in the AOT reversed micellar extraction, Bioseparation 6 (1997) 325-333.
20. D.G. Hayes, Mechanism of protein extraction from the solid state by water-in-oil microemulsions, Biotechnol. Bioeng. 53 (1997) 583-593.
21. V.M. Paradkar, J.S. Dordick, Mechanism of extraction of chymotrypsin into isooctane at very low concentrations of Aerosol OT in the absence of reverse micelles, Biotechnol. Bioeng. 43 (1994) 529-540.
22. A. Carlson, R. Nagarajan, Release and recovery of porcine pepsin and bovine chymosin from reverse micelles: A new technique based on isopropyl alcohol addition, Biotechnol. Prog. 8 (1992) 85-90.
23. G.J. Lye, J.A. Asenjo, D.L. Pyle, Extraction of lysozyme and ribonuclease-a using reverse micelles, Limits to protein solubilization, Biotechnol. Bioeng. 47 (1995) 509-519.
24. K. Maruyama, M. Imai, M. Shimizu, Molecular structure and solubilization state of proteins in AOT/isooctane reverse micellar system (in Japanese), In: The Division of 'Application of Liquid Membrane Techniques' under The SCEJ) Development of Liquid Membrane and Microemulsion Techniques, (Ed.), 1994, pp. 19-30.