Enrichment of carotenoids in flaxseed by introducing a bacterial phytoene synthase gene

Methods in Molecular Biology

Volumn 643, Issue , 2010, Pages 201-211

  Fujisawa, Masaki ^a   Misawa, Norihiko ^a  

^a NONE

Author keywords

Carotenoids; flax; linseed; phytoene synthase; provitamin A

Indexed keywords

CAROTENOID; PHYTOENE SYNTHASE; TRANSFERASE;

ARTICLE; BIOSYNTHESIS; ENZYMOLOGY; FLAX; GENETIC ENGINEERING; GENETIC TRANSFORMATION; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PANTOEA; PIGMENTATION; PLANT GROWTH; PLANT ROOT; PLANT SEED; RHIZOBIUM RADIOBACTER; TRANSGENIC PLANT;

ALKYL AND ARYL TRANSFERASES; CAROTENOIDS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; FLAX; GENETIC ENGINEERING; HYPOCOTYL; PANTOEA; PIGMENTATION; PLANT ROOTS; PLANTS, GENETICALLY MODIFIED; RHIZOBIUM RADIOBACTER; SEEDS; TRANSFORMATION, GENETIC;

EID: 77956907584     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60761-723-5_14     Document Type: Article

References (18)

1. Lichtenthaler, H. K., Schwender, J., Disch, A., and Rohmer, M. (1997) Biosynthesis of isoprenoids in higher plant chloroplasts proceeds via a mevalonate-independent pathway. FEBS Lett. 400, 271-274.
2. Al-Babili, S., and Beyer, P. (2005) Golden Rice - five years on the road - five years to go? Trends Plant Sci. 10, 565-573.
3. Nishino, H., Tokuda, H., Murakoshi, M., Satomi, Y., Masuda, M., Onozuka, M., Yamaguchi, S., Takayasu, J., Tsuruta, J., Okuda, M., Khachik, F., Narisawa, T., Takasuka, N., and Yano, M. (2000) Cancer prevention by natural carotenoids. Biofactors 13, 89-94.
4. Donaldson, M. S. (2004) Nutrition and cancer: A review of the evidence for an anticancer diet. Nutr. J. 3, 19.
5. Shewmaker, C. K., Sheehy, J. A., Daley, M., Colburn, S., and Ke, D. Y. (1999) Seedspecific overexpression of phytoene synthase: Increase in carotenoids and other metabolic effects. Plant J. 20, 401-412.
6. Burkhardt, P. K., Beyer, P., Wünn, J., Klöti, A., Armstrong, G. A., Schledz, M., von Lintig, J., and Potrykus, I. (1997) Transgenic rice (Oryza sativa) endosperm expressing daffodil (Narcissus pseudonarcissus) phytoene synthase accumulates phytoene, a key intermediate of provitamin A biosynthesis. Plant J. 11, 1071-1078.
7. Fraser, P. D., Römer, S., Shipton, C. A., Mills, P. B., Kiano, J. W., Misawa, N., Drake, R. G., Schuch, W., and Bramley, P. M. (2002) Evaluation of transgenic tomato plants expressing an additional phytoene synthase in a fruit-specific manner. P. Natl. Acad. Sci. U.S.A. 99, 1092-1097.
8. Giuliano, G., Bartley, G. E., and Scolnik, P. A. (1993) Regulation of carotenoid biosynthesis during tomato development. Plant Cell 5, 379-387.
9. Murashige, T., and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473-497.
10. Vervliet, G., Holsters, M., Teuchy, H., Van Montagu, M., and Schell, J. (1975) Characterization of different plaque-forming and defective temperate phages in Agrobacterium. J. Gen. Virol. 26, 33-48.
11. Fraser, P. D., Pinto, M. E., Holloway, D. E., and Bramley, P. M. (2000) Technical advance: Application of high-performance liquid chromatography with photodiode array detection to the metabolic profiling of plant isoprenoids. Plant J. 24, 551-558.
12. Fujisawa, M., Watanabe, M., Choi, S-K., Teramoto, M., Ohyama, K., and Misawa, N. (2008) Enrichment of carotenoids in flaxseed (Linum usitatissimum) by metabolic engineering with introduction of bacterial phytoene synthase gene crtB. J. Biosci. Bioeng. 105, 636-641.
13. Misawa, N., Satomi, Y., Kondo, K., Yokoyama, A., Kajiwara, S., Saito, T., Ohtani, T., and Miki, W. (1995) Structure and functional analysis of a marine bacterial carotenoid biosynthesis gene cluster and astaxanthin biosynthetic pathway proposed at the gene level. J. Bacteriol. 177, 6575-6584.
14. Misawa, N., Nakagawa, M., Kobayashi, K., Yamano, S., Izawa, Y., Nakamura, K., and Harashima, K. (1990) Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J. Bacteriol. 172, 6704-6712.
15. Misawa, N., Yamano, S., Linden, H., de Felipe, M. R., Lucas, M., Ikenaga, H., and Sandmann, G. (1993) Functional expression of the Erwinia uredovora carotenoid biosynthesis gene crtI in transgenic plants showing an increase of β-carotene biosynthesis activity and resistance to the bleaching herbicide norflurazon. Plant J. 4, 833-840.
16. Misawa, N., Masamoto, K., Hori, T., Ohtani, T., Boger, P., and Sandmann, G. (1994) Expression of an Erwinia phytoene desaturase gene not only confers multiple resistance to herbicides interfering with carotenoid biosynthesis but also alters xanthophyll metabolism in transgenic plants. Plant J. 6, 481-489.
17. Morris, W. L., Ducreux, L. J., Fraser, P. D., Millam, S., and Taylor, M. A. (2006) Engineering ketocarotenoid biosynthesis in potato tubers. Metab. Eng. 8, 253-263.
18. Rossak, M., Smith, M., and Kunst, L. (2001) Expression of the FAE1 gene and FAE1 promoter activity in developing seeds of Arabidopsis thaliana. Plant Mol. Biol. 46, 717-725.