Construction of an efficient Escherichia coli cell-free system for in vitro expression of several kinds of proteins

Engineering in Life Sciences

Volumn 10, Issue 4, 2010, Pages 333-338

  Ma, Rongrong ^a,b   Yang, Zhong ^a,c   Huang, Lei ^b   Zhu, Xiangcheng ^b   Kai, Lei ^b   Cai, Jin ^b   Wang, Xiaoning ^a,d   Xu, Zhinan ^b  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b ZHEJIANG UNIVERSITY   (China)

^c FUDAN UNIVERSITY   (China)

^d SOUTH CHINA UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

cAMP CP CK; Cell free protein synthesis; Energy regenerating system

Indexed keywords

AQUAPORINS; CAMP/CP/CK; CELL FREE PROTEIN SYNTHESIS; CELL-BASED; CELL-FREE; CELL-FREE SYSTEM; CREATINE KINASE; ENERGY-REGENERATING SYSTEM; ENHANCED GREEN FLUORESCENT PROTEIN; ESCHERICHIA COLI CELLS; EXPRESSION LEVELS; HIGH LEVEL EXPRESSION; HUMAN PROTEINS; IN-VITRO; MEMBRANE PROTEINS; MODEL PROTEINS; PROTEIN SYNTHESIS; SYNTHASES;

ADENOSINETRIPHOSPHATE; BIOLOGICAL MEMBRANES; ESCHERICHIA COLI; FETAL MONITORING; PHOSPHATES;

PROTEINS;

ADENOSINE TRIPHOSPHATE; CREATINE KINASE; CREATINE PHOSPHATE; CYCLIC AMP; ENHANCED GREEN FLUORESCENT PROTEIN; MEMBRANE PROTEIN;

COLIFORM BACTERIUM; ENZYME ACTIVITY; EXPERIMENTAL STUDY; GENE EXPRESSION; PROTEIN;

ARTICLE; CELL FREE SYSTEM; ESCHERICHIA COLI; IN VITRO STUDY; NONHUMAN; PROTEIN EXPRESSION; PROTEIN SYNTHESIS;

ESCHERICHIA COLI;

EID: 77956897772     PISSN: 16180240     EISSN: 16182863     Source Type: Journal    
DOI: 10.1002/elsc.201000052     Document Type: Article

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