Purification, characterization, and molecular cloning of a novel amine:pyruvate transaminase from Vibrio fluvialis JS17

Applied Microbiology and Biotechnology

Volumn 61, Issue 5-6, 2003, Pages 463-471

  Shin, J S ^a,b   Yun, H ^a   Jang, J W ^a   Park, I ^a   Kim, B G ^a  

^a Seoul National University   (South Korea)

^b California Institute of Technology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA METHYLBENZYLAMINE; AMINE:PYRUVATE TRANSAMINASE; AMINOTRANSFERASE; OMEGA AMINO ACID; PYRIDOXAL 5 PHOSPHATE; PYRUVIC ACID; UNCLASSIFIED DRUG;

ENZYME;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL STRAIN; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME PURIFICATION; ENZYME STABILITY; ENZYME STRUCTURE; GEL FILTRATION CHROMATOGRAPHY; GENETIC CONSERVATION; ISOELECTRIC POINT; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; PH; PROTEIN ANALYSIS; SEQUENCE HOMOLOGY; TEMPERATURE; VIBRIO FLUVIALIS;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; VIBRIO; VIBRIO FLUVIALIS;

EID: 0038236890     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-003-1250-6     Document Type: Article

References (28)

1. Bartsch K, Schneider R, Schulz A (1996) Stereospecific production of the herbicide phosphinothricin: purification of aspartate transaminase from Bacillus stearothermophilus, cloning of the corresponding gene, aspC, and application in a coupled transaminase process. Appl Environ Microbiol 62:3794-3799
2. Bierman J, Logan R, O'Brien K, Seno ET, Rao RN, Schoner BE (1992) Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. Gene 116:43-49
3. Chao Y-P, Lai ZJ, Chen P, Chern J-T (1999) Enhanced conversion rate of L-phenylalanine by coupling reactions of aminotransferases and phosphoenolpyruvate carboxykinase in Escherichia coli K-12. Biotechnol Prog 15:453-458
4. Christen P, Metzler DE (1985) Transaminases. Wiley, New York
5. Daniel MB, Edelstein SJ (1996) Protein methods. Wiley, New York
6. Dennis WH, Walters FH, Wilson TD, Stuart JD (1979) High performance liquid chromatographic determination of amino acids in the picomole range. Anal Chem 51:391-395
7. Higgins DG, Sharp PM (1988) CLUSTAL: a package for performing multiple sequence alignment on a microcomputer. Gene 73:237-244
8. Kaneko T, Nakamura Y, Sato S, Asamizu E, Kato T, Sasamoto S, Watanabe A, Idesawa K, Ishikawa A, Kawashima K, Kimura T, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Mochizuki Y, Nakayama S, Nakazaki N, Shimpo S, Sugimoto M, Takeuchi C, Yamada M, Tabata S (2000) Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti. DNA Res 7:331-338
9. Manning JM (1998) Substrate inhibition of D-amino acid transaminase and protection by salts and by reduced nicotinamide adenine dinucleotide: isolation and initial characterization of a pyridoxo intermediate related to inactivation. Biochemistry 37:2879-2888
10. Mehta PK, Christen P (1994) Homology of 1-aminocyclopropane-1-carboxylate synthase, D-amino-7-oxononanoate synthase, 2-amino-6-carprolactam racemase, 2,2-dialkylglycine decarboxylase, glutamate-1-semialdehyde 1,2-aminomutase and isopenicillin-N-epimerase with aminotransferases. Biochem Biophys Res Commun 198:138-143
11. Mehta PK, Hale TI, Christen P (1993) Aminotransferases: demonstration of homology and division into evolutionary subgroups. Eur J Biochem 214:549-569
12. Nierman WC, Feldblyum TV, Paulsen IT, Nelson KE, Eisen J, Heidelberg JF, Alley M, Ohta N, Maddock JR, Potocka I, Nelson WC, Newton A, Stephens C, Phadke N, Ely B, Laub MT, DeBoy RT, Dodson RJ, Durkin AS, Gwinn ML, Haft DH, Kolonay JF, Smit J, Craven M, Khouri H, Shetty J, Berry K, Utterback T, Tran K, Wolf A, Vamathevan J, Ermolaeva M, White O, Salzberg SL, Shapiro L, Venter JC, Fraser CM (2001) Complete genome sequence of Caulobacter crescentus. Proc Natl Acad Sci USA 98:4136-4141
13. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
14. Shen BW, Hennig M, Hohenester E, Jansonius JN, Schirmer T (1998) Crystal structure of human recombinant ornithine aminotransferase 277:81-102
15. Shin J-S, Kim B-G (1997) Kinetic resolution of α-methylbenzylamine with ω-transaminase screened from soil microorganisms: application of a biphasic system to overcome product inhibition. Biotechnol Bioeng 55:348-358
16. Shin J-S, Kim B-G (1998) Kinetic modeling of ω-transamination for enzymatic kinetic resolution of α-methylbenzylamine. Biotechnol Bioeng 60:534-540
17. Shin J-S, Kim B-G (1999) Asymmetric synthesis of chiral amines with ω-transaminase. Biotechnol Bioeng 65:206-211
18. Shin J-S, Kim B-G (2001) Comparison of the ω-transaminases from different microorganisms and application to production of chiral amines. Biosci Biotechnol Biochem 65:1782-1788
19. Shin J-S, Kim B-G, Liese A, Wandrey C (2001) Kinetic resolution of chiral amines using enzyme-membrane reactor. Biotechnol Bioeng 73:179-187
20. Stinson SC (1997) Chiral drug market shows signs of maturity. Chem Eng News October 20:38-70
21. Stirling DI (1992) The use of aminotransferases for the production of chiral amino acids and amines. In: Collins AN, Sheldrake GN, Crosby J (eds) Chirality in industry. Wiley, New York, p 209
22. Stover CK, Pham XQ, Erwin AL, Mizoguchi SD, Warrener P, Hickey MJ, Brinkman FS, Hufnagle WO, Kowalik DJ, Lagrou M, Garber RL, Goltry L, Tolentino E, Westbrock-Wadman S, Yuan Y, Brody LL, Coulter SN, Folger KR, Kas A, Larbig K, Lim R, Smith K, Spencer D, Wong GK, Wu Z, Paulsen IT, Reizer J, Saier MH, Hancock RE, Lory S, Olson MV (2000) Complete genome sequence of Pseudomonas aeruginosa PA01, an opportunistic pathogen. Nature 406:959-964
23. Taylor PP, Pantaleone DP, Senkpeil RF, Fotheringham IG (1998) Novel biosynthetic approaches to the production of unnatural amino acids using transaminases. Trends Biotechnol 16:412-418
24. Yonaha K, Yonaha S (1980) Aminobutyrate: α-ketoglutarate aminotransferase from Pseudomonas sp. F-126: purification, crystallization, and enzymologic properties. Arch Biochem Biophys 200:156-164
25. Yonaha K, Suzuki K, Minei H, Toyama S (1983a) Distribution of ω-amino acid: pyruvate transferase and aminobutyrate: α-ketoglutarate transaminase in microorganisms. Agric Biol Chem 47:2257-2265
26. Yonaha K, Toyama S, Kagamiyama H (1983b) Properties of the bound coenzyme and subunit structure of ω-amino acid:pyruvate aminotransferase. J Biol Chem 258:2260-2265
27. Yonaha K, Toyama S, Soda K (1987) ω-Amino acid-pyruvate aminotransferase. Methods Enzymol 143:500-505
28. Yonaha K, Nishie M, Aibara S (1992) The primary structure of omega-amino acid:pyruvate aminotransferase. J Biol Chem 267:12506-12510