An Inexpensive Simple-to-Use Inverted Fluorescence Microscope: A New Tool for Cellular Analysis

Journal of Laboratory Automation

Volumn 15, Issue 5, 2010, Pages 355-361

  Kahle, Jennifer ^a   Levin, Robert ^a   Niles, Walter ^a   Rasnow, Brian ^a   Schehlein, Mel ^a   Shumate, Chris ^a  

^a Etaluma Inc   (United States)

Author keywords

CMOS; fluorescent; inverted; LED; microscope; portable; USB

Indexed keywords

CMOS; FLUORESCENT; INVERTED MICROSCOPE; LED; PORTABLE MICROSCOPE; USB;

COSTS; FLUORESCENCE MICROSCOPY; LABORATORIES; MICROSCOPES;

FLUORESCENCE;

ANALYTIC METHOD; ARTICLE; BIOSENSOR; COMPUTER PROGRAM; COST; FLUORESCENCE IMAGING; FLUORESCENCE MICROSCOPE; FLUORESCENCE MICROSCOPY; IMAGE ANALYSIS; IMAGE PROCESSING; INVERTED FLUORESCENCE MICROSCOPE; LIGHT; LIGHT EMITTING DIODE; MASS SPECTROMETRY; SEMICONDUCTOR;

EID: 77956806215     PISSN: 22110682     EISSN: 15402452     Source Type: Journal    
DOI: 10.1016/j.jala.2010.06.008     Document Type: Article

References (27)

1. Wintergreen Research Worldwide optical, transmission TEM, and scanning SEM electron microscope market shares, forecasts, and strategies, 2009–2015. 2009, http://www.researchandmarkets.com/reports/686661 (accessed March 29, 2010).
2. Piston D. W. Davidson M. W. Multiphoton fluorescence microscopy. Olympus Microscopy Resource Center, Olympus America; 2009, http://www.olympusmicro.com/primer/techniques/fluorescence/multiphoton/multiphotonintro.html (accessed March 29, 2010).
3. Capps R. The good enough revolution: When cheap and simple is just fine. Wired Magazine 2009, 17. 9. http://www.wired.com/print/gadegets/miscellaneous/magazine/17-09/ff_goodenough (accessed September 1, 2009).
4. Updike S. J. Hicks G. P. The enzyme electrode. Nature 1967, 214, 986–988.
5. Chang K. W. Alsenberg S. Soeldner J. S. Hiebert J. M. Validation and bioengineering aspects of an implantable glucose sensor. Trans. Am. Soc. Artif. Intern. Organs. 1973, 19, 352–360.
6. Signostics. Signostics introduces the world's smallest ultrasound product for global demands of affordable healthcare. Signostics Press Release, October 5, 2009, http://www.signosticsmedical.com/press_release.php?id=4 (accessed March 29, 2010).
7. Hooke R. Micrographia: Physiological descriptions of minute bodies made by magnifying glasses, http://www.gutenberg.net/dirs/1/5/4/9/15491/15491-h/15491-h.htm (accessed March 29, 2010).
8. Smith J. L. The inverted microscope—A new form of microscope. Am. J. Sci. Arts 1852, 14, 233–241.
9. Heimstädt O. Das fluoreszenzmikroskop. Z Wiss Mikrosk 1911, 28, 330–337. Cited in: Rusk N. The fluorescence microscope. Nat Methods 2009, 1 October. doi: 10.1038/ncb1941.
10. Shumate C. Hoffman A. Instrumental considerations in high content screening. Combin. Chem. HTS 2009, 12, 888–898.
11. Decision News Media. Technological advances drive microscopy market, http://www.drugresearcher.com/Research-management (accessed March 29, 2010).
12. TriMark Publications. Microscopy markets. 2008, 1–10, http://www.trimarkpublications.com (accessed March 29, 2010).
13. Piribo. Microscopy: The global market. BCC Research 2007, http://www.piribo.com/publications/medical_devices/microscopy.html (accessed March 29, 2010).
14. Thompson C. Build it. Share it. Profit. Can open source hardware work?: A circuit board for the masses: The Arduino microcontroller. Wired Magazine 2008, 16. 11. http://www.wired.com/techbiz/startups/magazine/16-11/ff_openmanufacturing?currentPage=all#ixzz0jiKfzT4d (accessed March 30, 2010).
15. Metcalf M. M. An electric lamp for microscope illumination. Science 1902, 15, 937–939. doi: 10.1126/science.15.389.937.
16. Martin G. Agostini H. Hansen L. Light emitting diode microscope illumination for green fluorescent protein or fluorescein isothiocyanate epifluorescence. Biotechniques 2005, 38, 204–206.
17. Fossum E. R. CMOS image sensors: Electronic camera-on-a-chip. IEEE Trans Electron Devices 1997, 44, 1689–1698.
18. Cheng H.-Y. King Y.-C. A CMOS image sensor with dark-current cancellation and dynamic sensitivity operations. IEEE Trans Electron Devices 2003, 50, 91–95.
19. Barlow H. B. General principles: The senses considered as physical instruments In The Senses; Barlow H. B. Mollon J. D., Eds.; Cambridge University Press: Cambridge, UK; 1982; pp 24–25.
20. Sutter J. D. USB inventor is tech's unlikely ‘rock star’. CNN Press Release, February 4, 2010, http://www.cnn.com/2010/TECH/02/04/ajay.bhatt.usb.inventor/index.html (accessed March 29, 2010).
21. Saylor J. Cook B. USB 3.0 specification now available: completed specification marks beginning of SuperSpeed USB product development. Nereus for USB-IF Press Release, November 17, 2008. San Jose, CA, http://www.businesswire.com/news/home/20081117005474/en/USB-3.0-Specification (accessed March 29, 2010).
22. Hewlett-Packard Comp.; Intel Corp.; Microsoft Corp.; NEC Corp.; ST-NXP Wireless; Texas Instruments. Universal Serial Bus 3.0 Specification. 2008.
23. Raymond E. S. The Cathedral and the Bazaar. Version 3.0, September 11, 2003, http://www.catb.org/esr/writings/homesteading/cathedral-bazaar (accessed March 29, 2010).
24. Spring K. R. Lowry R. J. Characteristics of low-light level television cameras. Meth. Cell. Biol 1990, 29, 270–289.
25. Woo J.-M. Park H.-H. Min H. S. Park Y. J. Hong S.-M. Park C. H. Statistical analysis of random telegraph noise in CMOS image sensors In Proceedings of the International Conference on Simulation of Semi-conductor Processes and Devices; 2008; pp 77–80. doi: 10.1109/SISPAD.2008.4648241.
26. Niles W. D. Cohen F. S. Radiometric calibration of a video fluorescence microscope for the quantitative imaging of resonance energy transfer. Rev. Sci. Instrum 1995, 66, 3527–3536.
27. Hecht S. Shlaer S. Pirenne M. H. Energy, quanta, and vision. J. Gen. Physiol. 1942, 25, 819–840.