Substrate-specific binding and conformational changes involving Ser 313and transmembrane domain 8 of the human reduced folate carrier, as determined by site-directed mutagenesis and protein cross-linking

Biochemical Journal

Volumn 430, Issue 2, 2010, Pages 265-274

  Hou, Zhanjun ^a   Wu, Jianmei ^a   Ye, Jun ^a   Cherian, Christina ^a   Matherly, Larry H ^a  

^a WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Antifolate; Cross linking; Folate; Major facilitator superfamily; Mutagenesis; Oligomer; Reduced folate carrier; Transporter

Indexed keywords

ACTIVATION ANALYSIS; AMINO ACIDS; HYDROPHILICITY; MUTAGENESIS; PHOSPHATASES;

ANTIFOLATE; FOLATE; MAJOR FACILITATOR SUPERFAMILY; REDUCED FOLATE CARRIER; TRANSPORTER;

SUBSTRATES;

2 [5 [[(1,2 DIHYDRO 3 METHYL 1 OXOBENZO[F]QUINAZOLIN 9 YL)METHYL]AMINO] 1 OXO 2 ISOINDOLINYL]GLUTARIC ACID; ALANINE; CARBON; CYSTEINE; FOLIC ACID; FOLIC ACID ANTAGONIST; FOLINIC ACID; GLYCOPROTEIN; LOMETREXOL; MEMBRANE PROTEIN; METHOTREXATE; NALPHA (4 AMINO 4 DEOXYPTEROYL) NDELTA HEMIPHTHALOYLORNITHINE; NITROGEN; PEMETREXED; PHENYLALANINE; PLEVITREXED; RALTITREXED; REDUCED FOLATE CARRIER; SERINE; THREONINE; TRANSMEMBRANE DOMAIN 1 PROTEIN; TRANSMEMBRANE DOMAIN 10 PROTEIN; TRANSMEMBRANE DOMAIN 11 PROTEIN; TRANSMEMBRANE DOMAIN 12 PROTEIN; TRANSMEMBRANE DOMAIN 2 PROTEIN; TRANSMEMBRANE DOMAIN 3 PROTEIN; TRANSMEMBRANE DOMAIN 4 PROTEIN; TRANSMEMBRANE DOMAIN 5 PROTEIN; TRANSMEMBRANE DOMAIN 6 PROTEIN; TRANSMEMBRANE DOMAIN 7 PROTEIN; TRANSMEMBRANE DOMAIN 8 PROTEIN; TRANSMEMBRANE DOMAIN 9 PROTEIN; UNCLASSIFIED DRUG; UNINDEXED DRUG; CARRIER PROTEIN; CROSS LINKING REAGENT; PROTEIN BINDING; SLC19A1 PROTEIN, HUMAN;

AMINO ACID SUBSTITUTION; ANTINEOPLASTIC ACTIVITY; ARTICLE; CELL LINE; CHEMICAL BOND; CONTROLLED STUDY; CROSS LINKING; DRUG BLOOD LEVEL; DRUG PROTEIN BINDING; DRUG TRANSPORT; ENZYME SPECIFICITY; FEMALE; GENE EXPRESSION; GENETIC TRANSFECTION; HELA CELL; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; MAXIMUM PLASMA CONCENTRATION; MOLECULE; MUTANT; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS; WILD TYPE; AMINO ACID SEQUENCE; CHEMICAL STRUCTURE; CHEMISTRY; GENETICS; KINETICS; METABOLISM; MOLECULAR GENETICS; PROTEIN SECONDARY STRUCTURE;

AMINO ACID SEQUENCE; CROSS-LINKING REAGENTS; HUMANS; KINETICS; MEMBRANE TRANSPORT PROTEINS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; PROTEIN STRUCTURE, SECONDARY; REDUCED FOLATE CARRIER PROTEIN; SUBSTRATE SPECIFICITY;

EID: 77956675864     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20100181     Document Type: Article

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