Engineering of a wheat germ expression system to provide compatibility with a high throughput pET-based cloning platform

Journal of Structural and Functional Genomics

Volumn 11, Issue 3, 2010, Pages 201-209

  Zhao, Li ^a,b   Zhao, Kate Q ^c   Hurst, Robin ^c   Slater, Michael R ^c   Acton, Thomas B ^a,b   Swapna, G V T ^a,b   Shastry, Ritu ^a,b   Kornhaber, Gregory J ^a,b   Montelione, Gaetano T ^a,b  

^a RUTGERS UNIVERSITY   (United States)

^b Northeast Structural Genomics Consortium   (United States)

^c PROMEGA CORPORATION   (United States)

Author keywords

Eukaryotic protein expression; Isotope enrichment; Micro cryo NMR probe; Structural genomics; Wheat germ cell free expression system

Indexed keywords

CARBON 13; NITROGEN 15;

ARTICLE; CELL FREE SYSTEM; ESCHERICHIA COLI; EUKARYOTE; EXPRESSION VECTOR; GENE EXPRESSION SYSTEM; GENETIC ENGINEERING; HIGH THROUGHPUT SCREENING; MOLECULAR CLONING; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; SCALE UP; STRUCTURAL GENOMICS; STRUCTURE ANALYSIS; WHEAT GERM;

CELL-FREE SYSTEM; CLONING, MOLECULAR; ESCHERICHIA COLI; GENETIC VECTORS; HUMANS; PROTEIN ENGINEERING; PROTEINS; PROTEOMICS; RECOMBINANT PROTEINS;

ESCHERICHIA COLI; EUKARYOTA; TRITICUM AESTIVUM;

EID: 77956614932     PISSN: 1345711X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10969-010-9093-8     Document Type: Article

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