Immortalized mouse floxed Bmp2 dental papilla mesenchymal cell lines preserve odontoblastic phenotype and respond to BMP2

Journal of Cellular Physiology

Volumn 225, Issue 1, 2010, Pages 132-139

  Wu, Li An ^a,b   Feng, Junsheng ^a,c   Wang, Lynn ^a   Mu, Yan Dong ^a   Baker, Andrew ^a   Donly, Kevin J ^a   Gluhak Heinrich, Jelica ^a   Harris, Stephen E ^a   Macdougall, Mary ^d   Chen, Shuo ^a  

^a UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO   (United States)

^b FOURTH MILITARY MEDICAL UNIVERSITY   (China)

^c FUJIAN MEDICAL UNIVERSITY   (China)

^d UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PHOSPHATASE; BONE MORPHOGENETIC PROTEIN 2;

ANIMAL CELL; ARTICLE; BONE MINERALIZATION; CELL CLONING; CELL CULTURE; CELL DIFFERENTIATION; CELL IMMORTALIZATION; CELL ISOLATION; CONTROLLED STUDY; DENTIN; GENETIC TRANSFECTION; IMMUNOHISTOCHEMISTRY; MESENCHYMAL STEM CELL; MOLAR TOOTH; MOUSE; NONHUMAN; ODONTOBLAST; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TOOTH DEVELOPMENT; TOOTH PAPILLA;

ANIMALS; BIOLOGICAL MARKERS; BONE MORPHOGENETIC PROTEIN 2; CALCIFICATION, PHYSIOLOGIC; CELL DIFFERENTIATION; CELL LINE; CELL SHAPE; DENTAL PAPILLA; MESENCHYMAL STEM CELLS; MICE; MICE, TRANSGENIC; ODONTOBLASTS; PHENOTYPE;

EID: 77956543579     PISSN: 00219541     EISSN: 10974652     Source Type: Journal    
DOI: 10.1002/jcp.22204     Document Type: Article

References (62)

1. Ameri K, Harris AL. 2008. Activating transcription factor 4. Int J Biochem Cell Biol 40:14-21.
2. Bandyopadhyay A, Tsuji K, Cox K, Harfe BD, Rosen V, Tabin CJ. 2006. Genetic analysis of the roles of BMP2, BMP4, and BMP7 in limb patterning and skeletogenesis. PLoS Genet 2:e216.
3. Chen D, Zhao M, Mundy GR. 2004. Bone morphogenetic proteins. Growth Factors 22:233-241.
4. Chen S, Rani S, Wu Y, Unterbrink A, Gu TT, Gluhak-Heinrich J, Chuang HH, Macdougall M. 2005. Differential regulation of dentin sialophosphoprotein expression by Runx2 during odontoblast cytodifferentiation. J Biol Chem 280:29717-29727.
5. Chen S, Gluhak-Heinrich J, Martinez M, Li T, Wu Y, Chuang HH, Chen L, Dong J, Gay I, MacDougall M. 2008. Bone morphogenetic protein 2 mediates dentin sialophosphoprotein expression and odontoblast differentiation via NF-Y signaling. J Biol Chem 283:19359-19370.
6. Dong J, Amor D, Aldred MJ, Gu T, Escamilla M, MacDougall M. 2005. DLX3 mutation associated with autosomal dominant amelogenesis imperfecta with taurodontism. Am J Med Genet A 133A:138-141. (Pubitemid 40279779)
7. D'Souza RN, Cavender A, Sunavala G, Alvarez J, Ohshima T, Kulkarni AB, MacDougall M. 1997. Gene expression patterns of murine dentin matrix protein 1 (Dmp1) and dentin sialophosphoprotein (DSPP) suggest distinct developmental functions in vivo. J Bone Miner Res 12:2040-2049.
8. D'Souza RN, Aberg T, Gaikwad J, Cavender A, Owen M, Karsenty G, Thesleff I. 1999. Cbfa1 is required for epithelial-mesenchymal interactions regulating tooth development in mice. Development 126:2911-2920.
9. Ducy P, Karsenty G. 2000. The family of bone morphogenetic proteins. Kidney Int 57:2207-2214.
10. Ducy P, Zhang R, Geoffroy V, Ridall AL, Karsenty G. 1997. Osf2/Cbfa1: A transcriptional activator of osteoblast differentiation. Cell 89:747-754. (Pubitemid 27516177)
11. Duverger O, Lee D, Hassan MQ, Chen SX, Jaisser F, Lian JB, Morasso MI. 2008. Molecular consequences of a frameshifted DLX3 mutant leading to Tricho-Dento-Osseous syndrome. J Biol Chem 283:20198-20208.
12. Fei Y, Xiao L, Hurley MM. 2010. Fibroblast growth factor 2 positively regulates expression of activating transcription factor 4 in osteoblasts. Biochem Biophys Res Commun 391:335-339.
13. Gagneten S, Le Y, Miller J, Sauer B. 1997. Brief expression of a GFP cre fusion gene in embryonic stem cells allows rapid retrieval of site-specific genomic deletions. Nucleic Acids Res 25:3326-3331. (Pubitemid 27338694)
14. Galler KM, Schweikl H, Thonemann B, D'Souza RN, Schmalz G. 2006. Human pulp-derived cells immortalized with Simian Virus 40 T-antigen. Eur J Oral Sci 114:138-146.
15. Hanks CT, Sun ZL, Fang DN, Edwards CA, Wataha JC, Ritchie HH, Butler WT. 1998. Cloned 3T6 cell line from CD-1 mouse fetal molar dental papillae. Connect Tissue Res 37:233-249.
16. Hart PS, Hart TC. 2007. Disorders of human dentin. Cells Tissues Organs 186:70-77.
17. Hayashi Y, Imai M, Goto Y, Murakami N. 1993. Pathological mineralization in a serially passaged cell line from rat pulp. J Oral Pathol Med 22:175-179.
18. Hogan BL. 1996. Bone morphogenetic proteins: Multifunctional regulators of vertebrate development. Genes Dev 10:1580-1594. (Pubitemid 26268864)
19. Iwata T, Yamakoshi Y, Simmer JP, Ishikawa I, Hu JC. 2007. Establishment of porcine pulp-derived cell lines and expression of recombinant dentin sialoprotein and recombinant dentin matrix protein-1. Eur J Oral Sci 115:48-56.
20. Javed A, Bae JS, Afzal F, Gutierrez S, Pratap J, Zaidi SK, Lou Y, van Wijnen AJ, Stein JL, Stein GS, Lian JB. 2008. Structural coupling of Smad and Runx2 for execution of the BMP2 osteogenic signal. J Biol Chem 283:8412-8422.
21. Kim JW, Simmer JP. 2007. Hereditary dentin defects. J Dent Res 86:392-399.
22. Komori T, Yagi H, Nomura S, Yamaguchi A, Sasaki K, Deguchi K, Shimizu Y, Bronson RT, Gao YH, Inada M, Sato M, Okamoto R, Kitamura Y, Yoshiki S, Kishimoto T. 1997. Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts. Cell 89:755-764.
23. Lee B, Thirunavukkarasu K, Zhou L, Pastore L, Baldini A, Hecht J, Geoffroy V, Ducy P, Karsenty G. 1997. Missense mutations abolishing DNA binding of the osteoblast-specific transcription factor OSF2/CBFA1 in cleidocranial dysplasia. Nat Genet 16:307-310.
24. Lee KY, Jeong JW, Wang J, Ma L, Martin JF, Tsai SY, Lydon JP, DeMayo FJ. 2007. Bmp2 is critical for the murine uterine decidual response. Mol Cell Biol 27:5468-5478.
25. Linde A, Goldberg M. 1993. Dentiogenesis. Crit Rev Oral Biol Med 4:679-728.
26. Ma L, Martin JF. 2005. Generation of a Bmp2 conditional null allele. Genesis 42:203-206. (Pubitemid 41099679)
27. Ma L, Lu MF, Schwartz RJ, Martin JF. 2005. Bmp2 is essential for cardiac cushion epithelial-mesenchymal transition and myocardial patterning. Development 132:5601-5611.
28. MacDougall M, Thiemann F, Ta H, Hsu P, Chen LS, Snead ML. 1995. Temperature sensitive simian virus 40 large T antigen immortalization of murine odontoblast cell cultures: Establishment of clonal odontoblast cell line. Connect Tissue Res 33:97-103.
29. MacDougall M, Dong J, Acevedo AC. 2006. Molecular basis of human dentin diseases. Am J Med Genet A 140:2536-2546. (Pubitemid 44865081)
30. Matsubara T, Kida K, Yamaguchi A, Hata K, Ichida F, Meguro H, Aburatani H, Nishimura R, Yoneda T. 2008. BMP2 regulates Osterix through Msx2 and Runx2 during osteoblast differentiation. J Biol Chem 283:29119-29125.
31. Matsuda T, Cepko CL. 2007. Controlled expression of transgenes introduced by in vivo electroporation. Proc Natl Acad Sci USA 104:1027-1032.
32. Mundlos S, Otto F, Mundlos C, Mulliken JB, Aylsworth AS, Albright S, Lindhout D, Cole WG, Henn W, Knoll JH, Owen MJ, Mertelsmann R, Zabel BU, Olsen BR. 1997. Mutations involving the transcription factor CBFA1 cause cleidocranial dysplasia. Cell 89:773-779.
33. Nagata T, Yokota M, Ohishi K, Nishikawa S, Shinohara H, Wakano Y, Ishida H. 1994. 1 alpha,25-dihydroxyvitamin D3 stimulation of osteopontin expression in rat clonal dental pulp cells. Arch Oral Biol 39:775-782. (Pubitemid 124034556)
34. Nakashima M. 2005. Bone morphogenetic proteins in dentin regeneration for potential use in endodontic therapy. Cytokine Growth Factor Rev 16:369-376.
35. Nakashima K, Zhou X, Kunkel G, Zhang Z, Deng JM, Behringer RR, de Crombrugghe B. 2002. The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation. Cell 108:17-29.
36. Otto F, Thornell AP, Crompton T, Denzel A, Gilmour KC, Rosewell IR, Stamp GW, Beddington RS, Mundlos S, Olsen BR, Selby PB, Owen MJ. 1997. Cbfa1, a candidate gene for cleidocranial dysplasia syndrome, is essential for osteoblast differentiation and bone development. Cell 89:765-771.
37. Panagakos FS. 1998. Transformation and preliminary characterization of primary human pulp cells. J Endod 24:171-175.
38. Park GT, Morasso MI. 2002. Bone morphogenetic protein-2 (BMP-2) transactivates Dlx3 through Smad1 and Smad4: Alternative mode for Dlx3 induction in mouse keratinocytes. Nucleic Acids Res 30:515-522.
39. Priam F, Ronco V, Locker M, Bourd K, Bonnefoix M, Duchêne T, Bitard J, Wurtz T, Kellermann O, Goldberg M, Poliard A. 2005. New cellular models for tracking the odontoblast phenotype. Arch Oral Biol 50:271-277.
40. Price JA, Bowden DW, Wright JT, Pettenati MJ, Hart TC. 1998. Identification of a mutation in DLX3 associated with tricho-dento-osseous (TDO) syndrome. Hum Mol Genet 7:563-569.
41. Quack I, Vonderstrass B, Stock M, Aylsworth AS, Becker A, Brueton L, Lee PJ, Majewski F, Mulliken JB, Suri M, Zenker M, Mundlos S, Otto F. 1999. Mutation analysis of core binding factor A1 in patients with cleidocranial dysplasia. Am J Hum Genet 65:1268-1278.
42. Rajpar MH, Koch MJ, Davies RM, Mellody KT, Kielty CM, Dixon MJ. 2002. Mutation of the signal peptide region of the bicistronic gene DSPP affects translocation to the endoplasmic reticulum and results in defective dentine biomineralization. Hum Mol Genet 11:2559-2565.
43. Reddi AH. 1997. Bone morphogenetic proteins: An unconventional approach to isolation of first mammalian morphogens. Cytokine Growth Factor Rev 8:11-20.
44. Ritchie HH, Hou H, Veis A, Butler WT. 1994. Cloning and sequence determination of rat dentin sialoprotein, a novel dentin protein. J Biol Chem 269:3698-3702. (Pubitemid 24237697)
45. Rivera-Feliciano J, Tabin CJ. 2006. Bmp2 instructs cardiac progenitors to form the heart-valve-inducing field. Dev Biol 295:580-588.
46. Rosen V. 2009. BMP2 signaling in bone development and repair. Cytokine Growth Factor Rev 20:475-480.
47. Singh AP, Castranio T, Scott G, Guo D, Harris MA, Ray M, Harris SE, Mishina Y. 2008. Influences of reduced expression of maternal bone morphogenetic protein 2 on mouse embryonic development. Sex Dev 3:134-141.
48. Suguro H, Asano M, Kaneko Y, Omagari D, Ogiso B, Moro I, Komiyama K. 2008. Characterization of human dental pulp-derived cell lines. Int Endod J 41:609-616.
49. Thesleff I. 2003. Epithelial-mesenchymal signalling regulating tooth morphogenesis. J Cell Sci 116:1647-1648.
50. Thonemann B, Schmalz G. 2000. Immortalization of bovine dental papilla cells with simian virus 40 large T antigen. Arch Oral Biol 45:857-869.
51. Tsuji K, Bandyopadhyay A, Harfe BD, Cox K, Kakar S, Gerstenfeld L, Einhorn T, Tabin CJ, Rosen V. 2006. BMP2 activity, although dispensable for bone formation, is required for the initiation of fracture healing. Nat Genet 38:1424-1429.
52. Ulsamer A, Ortuño MJ, Ruiz S, Susperregui AR, Osses N, Rosa JL, Ventura F. 2008. BMP-2 induces Osterix expression through up-regulation of Dlx5 and its phosphorylation by p38. Biol Chem 283:3816-3826.
53. Urist MR. 1965. Bone: Formation by autoinduction. Science 150:893-899.
54. Wozney JM, Rosen V, Celeste AJ, Mitsock LM, Whitters MJ, Kriz R, Hewick R, Wang EA. 1988. Novel regulators of bone formation: Molecular clones and activities. Science 242:1528-1534.
55. Wright JT, Hong SP, Simmons D, Daly B, Uebelhart D, Luder HU. 2008. DLX3 c.561-562delCT mutation causes attenuated phenotype of tricho-dento-osseous syndrome. Am J Med Genet A 146:343-349. (Pubitemid 351212835)
56. Wu XB, Schneider A, Yu W, Rajendren Iqbal GJ, Yamamoto M, Alam M, Brunet LJ, Blair HC, Zaidi M, Abe E. 2003. Impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis in noggin-overexpressing mice. J Clin Investig 112:924-993.
57. Wu LA, Yuan G, Yang G, Ortiz-Gonzalez I, Yang W, Cui Y, MacDougall M, Donly KJ, Harris S, Chen S. 2009. Immortalization and characterization of mouse floxed Bmp2/4 osteoblasts. Biochem Biophys Res Commun 386:89-95.
58. Xiao S, Yu C, Chou X, Yuan W, Wang Y, Bu L, Fu G, Qian M, Yang J, Shi Y, Hu L, Han B, Wang Z, Huang W, Liu J, Chen Z, Zhao G, Kong X. 2001. Dentinogenesis imperfecta 1 with or without progressive hearing loss is associated with distinct mutations in DSPP. Nat Genet 27:201-204.
59. Xiao G, Jiang D, Ge C, Zhao Z, Lai Y, Boules H, Phimphilai M, Yang X, Karsenty G, Franceschi RT. 2005. Cooperative interactions between activating transcription factor 4 and Runx2/Cbfa1 stimulate osteoblast-specific osteocalcin gene expression. J Biol Chem 280:30689-30696.
60. Yang X, Matsuda K, Bialek P, Jacquot S, Masuoka HC, Schinke T, Li L, Brancorsini S, Sassone-Corsi P, Townes TM, Hanauer A, Karsenty G. 2004. ATF4 is a substrate of RSK2 and an essential regulator of osteoblast biology: Implication for Coffin-Lowry Syndrome. Cell 117:387-398.
61. Yang X, van der Kraan PM, Bian Z, Fan M, Walboomers XF, Jansen JA. 2009. Mineralized tissue formation by BMP2-transfected pulp stem cells. J Dent Res 88:1020-1025.
62. Zhang H, Bradley A. 1996. Mice deficient for BMP2 are nonviable and have defects in amnion/chorion and cardiac development. Development 122:2977-2986. (Pubitemid 26371964)