Monitoring the plant epiphyte Methylobacterium extorquens DSM 21961 by real-time PCR and its influence on the strawberry flavor

FEMS Microbiology Ecology

Volumn 74, Issue 1, 2010, Pages 136-145

  Verginer, Markus ^a   Siegmund, Barbara ^a   Cardinale, Massimiliano ^a   Müller, Henry ^a   Choi, Young ^b   Míguez, Carlos B ^b   Leitner, Erich ^a   Berg, Gabriele ^a  

^a GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

Methylobacterium extorquens; monitoring; qRT PCR; strawberry leaves

Indexed keywords

4 METHOXY 2,5 DIMETHYL 3(2H) FURANONE; 4-METHOXY-2,5-DIMETHYL-3(2H)-FURANONE; BACTERIAL DNA; DRUG DERIVATIVE; FURAN DERIVATIVE; FURANEOL; GAMMA BUTYROLACTONE;

BACTERIUM; BIOTECHNOLOGY; COLONIZATION; EPIPHYTE; FRUIT; KETONE; LEAF; MONITORING SYSTEM; NUTRITIVE VALUE; POLYMERASE CHAIN REACTION; REAL TIME; TRICHOME;

ARTICLE; CONFOCAL MICROSCOPY; GAS CHROMATOGRAPHY; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; LIMIT OF DETECTION; METABOLISM; METHYLOBACTERIUM EXTORQUENS; MICROBIOLOGY; PLANT LEAF; POLYMERASE CHAIN REACTION; STRAWBERRY;

4-BUTYROLACTONE; CHROMATOGRAPHY, GAS; DNA, BACTERIAL; FRAGARIA; FURANS; LIMIT OF DETECTION; METHYLOBACTERIUM EXTORQUENS; MICROSCOPY, CONFOCAL; PLANT LEAVES; POLYMERASE CHAIN REACTION;

BACTERIA (MICROORGANISMS); FRAGARIA X ANANASSA; METHYLOBACTERIUM EXTORQUENS;

EID: 77956501566     PISSN: 01686496     EISSN: 15746941     Source Type: Journal    
DOI: 10.1111/j.1574-6941.2010.00942.x     Document Type: Article

References (29)

1. Abbasi PA, Miller SA, Meulia T, Hoitink HA Kim JM (1999) Precise detection and tracing of Trichoderma hamatum 382 in compost-amended potting mixes by using molecular markers. Appl Environ Microb 65 : 5421 5426.
2. Altschul SF, Madden TL, Schaffer AA, Zhang J, Miller W Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25 : 3389 3402.
3. Berg G (2009) Plant-microbe interactions promoting plant growth and health: perspectives for controlled use of microorganisms in agriculture. Appl Microbiol Biot 84 : 11 18.
4. Bood KG Zabetakis I (2002) The biosynthesis of strawberry flavor (II): biosynthetic and molecular biology studies. J Food Sci 67 : 2 8.
5. Bourque D, Pomerlau Y Groleau D (1995) High-cell-density production of poly-β-hydroxybutyrate (PHB) from methanol by Methylobacterium extorquens: production of high-molecular-mass PHB. Appl Microbiol Biot 44 : 367 376.
6. Choi YJ, Bourques D, Morel L, Groleau D Miguez CB (2006) Multicopy integration and expression of heterologous genes in Methylobacterium extorquens ATCC 55366. Appl Environ Microb 72 : 753 759.
7. Delmotte N, Knief C, Chaffron S, Innerebner G, Roschitzki B, Schlapbach R, von Mering C Vorholt JA (2009) Community proteogenomics reveals insights into the physiology of phyllosphere bacteria. P Natl Acad Sci USA 22 : 16428 16433.
8. Hadrys H, Ballick M Schierwater B (1992) Applications of random amplified polymorphic DNA (RAPD) in molecular ecology. Mol Ecol 1 : 55 63.
9. Hallmann J Berg G (2006) Spectrum and population dynamics of root endophytes. Microbial Root Endophytes (Schulz B, Boyle C Sieber T, eds), pp. 15 32. Springer Verlag, Berlin.
10. Hristova KR, Lutenegger CM Scow KM (2001) Detection and quantification of methyl tert-butyl ether-degrading strain PM1 by real-time TaqMan PCR. Appl Environ Microb 67 : 5154 5160.
11. Koeuth T, Versalovic J Lupski JR (1995) Differential subsequence conservation of interspersed repetitive Streptococcus pneumoniae BOX elements in diverse bacteria. Genome Res 5 : 408 418.
12. Labbe D, Rytz A, Morgenegg C, Ali S Martin N (2007) Subthreshold olfactory stimulation can enhance sweetness. Chem Senses 32 : 205 214.
13. Larsen M, Poll L Olsen CE (1992) Evaluation of the aroma composition of some strawberry (Fragaria ananassa Duch.) cultivars by use of odour threshold values. Z Lebensm Unters F A 195 : 536 539.
14. Lloyd-Jones G, Laurie AD Tizzard AD (2005) Quantification of the Pseudomonas population in New Zealand soils by fluorogenic PCR assay and culturing techniques. J Microbiol Meth 60 : 217 224.
15. Lugtenberg B Kamilova F (2009) Plant-growth-promoting rhizobacteria. Annu Rev Microbiol 63 : 541 556.
16. Pujol M, Badosa E, Cabrefiga J Montesinos E (2005) Development of a strain-specific quantitative method for monitoring Pseudomonas fluorescens EPS62e, a novel biocontrol agent of fire blight. FEMS Microbiol Lett 249 : 343 352.
17. Pujol M, Badosa E, Manceau C Montesinos E (2006) Assessment of the environmental fate of the biological control agent Pseudomonas fluorescens EPS62e on apple by culture and real-time PCR methods. Appl Environ Microb 72 : 2421 2427.
18. Salm H Geider K (2004) Real-time PCR for detection and quantification of Erwinia amylovora, the causal agent of fireblight. Plant Pathol 53 : 602 610.
19. Sambrook J Russel DW (2001) Molecular Cloning, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
20. Schena L, Finetti Sialer M Gallitelli D (2002) Molecular detection of strain L47 of Aureobasidium pullulans, a biocontrol agent of postharvest diseases. Plant Dis 86 : 54 60.
21. Schieberle P Hofmann T (1997) Evaluation of the character impact odorants in fresh strawberry juice by quantitative measurements and sensory studies on model mixtures. J Agric Food Chem 45 : 227 232.
22. ® and mesifuran in strawberries - an analytical challenge. Expression of Multidisciplinary Flavour Science (Blank I, Wüst M Yeretzian C, eds), pp. 537 540. ZHAW Züricher Hochschule für Angewandte Wissenschaften, Winterthur.
23. Sung WS, Jung HJ, Park K, Kim HS, Lee IS Lee DG (2006) 2,5-dimethyl-4-hydroxy-3(2H)-furanone (DMHF); antimicrobial compound with cell cycle arrest in nosocomial pathogens. Life Sci 80 : 586 591.
24. Welsh J McClelland M (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18 : 7213 7218.
25. Whipps JM, Hand P, Pink D Bending GD (2008) Phyllosphere microbiology with special reference to diversity and plant genotype. J Appl Microbiol 105 : 1744 1755.
26. Williams JGK, Kubelik AR, Kenneth JL, Rafalski JA Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18 : 6531 6535.
27. Zabetakis I (1997) Enhancement of flavour biosynthesis from strawberry (Fragaria x ananassa) callus cultures by Methylobacterium species. Plant Cell Tiss Org 50 : 179 183.
28. Zabetakis I Gramshaw JW (1996) 1,2-Propanediol in strawberries and its role as a flavour precursor. Food Chem 61 : 351 354.
29. Zabetakis I, Moutevelis-Minakakis P Gramshaw JW (1999) The role of 2-hydroxypropanal in the biosynthesis of 2,5-dimethyl-4-hydroxy-2H-furan-3-one in strawberry (Fragaria x ananassa, cv. Elsanta) callus cultures. Food Chem 64 : 311 314.