Epitope mapping using gram-positive surface display

Current Protocols in Immunology

Volumn , Issue SUPPL. 90, 2010, Pages

  Rockberg, Johan ^a   Löfblom, John ^a   Hjelm, Barbara ^a   Ståhl, Stefan ^a   Uhlén, Mathias ^a  

^a ALBANOVA UNIVERSITY CENTER   (Sweden)

Author keywords

antibody; cell surface; epitope; epitope mapping; Gram positive; Staphylococcus carnosus

Indexed keywords

MONOCLONAL ANTIBODY; POLYCLONAL ANTIBODY;

ANTIBODY LABELING; ANTIGEN BINDING; ANTIGEN EXPRESSION; ANTIGEN SPECIFICITY; BACTERIUM TRANSFORMATION; CELL STRUCTURE; CELL SURFACE; DNA FRAGMENTATION; DNA LIBRARY; DNA REPAIR; ELECTROPORATION; EPITOPE MAPPING; ESCHERICHIA COLI; GENE AMPLIFICATION; HUMAN; IMAGE DISPLAY; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PYROSEQUENCING; REVIEW; SEQUENCE ALIGNMENT; STAPHYLOCOCCUS CARNOSUS;

ANIMALS; CELL MEMBRANE; EPITOPE MAPPING; EPITOPES; GRAM-POSITIVE BACTERIA; HUMANS; IMMUNOCHEMISTRY; PATHOLOGY, MOLECULAR; PEPTIDE LIBRARY; TRANSGENES;

POSIBACTERIA; STAPHYLOCOCCUS CARNOSUS;

EID: 77956490637     PISSN: 19343671     EISSN: 1934368X     Source Type: Journal    
DOI: 10.1002/0471142735.im0909s90     Document Type: Review

References (10)

1. Augustin, J., and, Gtz, F., 1990. Transformation of Staphylococcus epidermidis and other staphylococcal species with plasmid DNA by electroporation. FEMS Microbiol. Lett. 54: 203 - 207.
2. Chao, G., Cochran, J.R., and, Wittrup, K.D., 2004. Fine epitope mapping of anti-epidermal growth factor receptor antibodies through random mutagenesis and yeast surface display. J. Mol. Biol. 342: 539 - 550.
3. Fack, F., Hugle-Dorr, B., Song, D., Queitsch, I., Petersen, G., and, Bautz, E.K., 1997. Epitope mapping by phage display. Random versus gene-fragment libraries. J. Immunol. Methods 206: 43 - 52.
4. Harvey, B.R., Georgiou, G., Hayhurst, A., Jeong, K.J., Iverson, B.L., and, Rogers, G.K., 2004. Anchored periplasmic expression, a versatile technology for the isolation of high-affinity antibodies from Escherichia coliexpressed libraries. Proc. Natl. Acad. Sci. U.S.A. 101: 9193 - 9198.
5. Hilpert, K., Winkler, D.F., and, Hancock, R.E., 2007. Peptide arrays on cellulose support: SPOT synthesis, a time and cost efficient method for synthesis of large numbers of peptides in a parallel and addressable fashion. Nat. Protoc. 2: 1333 - 1349.
6. Kronqvist, N., Lfblom, J., Jonsson, A., Wernerus, H., and, Sthl, S., 2008. A novel affinity protein selection system based on staphylococcal cell surface display and flow cytometry. Protein Eng. Des. Sel. 21: 247 - 255.
7. Lfblom, J., Kronqvist, N., Uhlen, M., Sthl, S., and, Wernerus, H., 2007. Optimization of electroporation-mediated transformation: Staphylococcus carnosus as model organism. J. Appl. Microbiol. 102: 736 - 747.
8. Rockberg, J., Lfblom, J., Hjelm, B., Uhlen, M., and, Sthl, S., 2008. Epitope mapping of antibodies using bacterial surface display. Nat. Methods 5: 1039 - 1045.
9. van Zonneveld, A.J., van den Berg, B.M., van Meijer, M., and, Pannekoek, H., 1995. Identification of functional interaction sites on proteins using bacteriophage-displayed random epitope libraries. Gene 167: 49 - 52.
10. Rockberg et al., 2008. See above. Describes method for epitope mapping of antibodies using S. carnosus cell surface display including results with mapping of 15 monoclonal and polyclonal antibodies.