Chromosome integration of BAC (bacterial artificial chromosome): Evidence of multiple rearrangements

Transgenic Research

Volumn 19, Issue 5, 2010, Pages 923-931

  Le Saux, Agnès ^a   Houdebine, Louis Marie ^a   Jolivet, Geneviève ^a  

^a ECOLE NATIONALE VÉTÉRINAIRE D ALFORT   (France)

Author keywords

Bacterial artificial chromosome; Integration site; TAIL PCR; Thermal asymmetric interlaced PCR; Transgene

Indexed keywords

MILK PROTEIN; RECOMBINANT DNA; WHEY ACIDIC PROTEINS;

ANIMAL; ARTICLE; BACTERIAL ARTIFICIAL CHROMOSOME; C57BL MOUSE; CBA MOUSE; CHROMOSOME; GENETIC RECOMBINATION; GENETICS; METHODOLOGY; MICROINJECTION; MOLECULAR GENETICS; MOUSE; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY; SWINE; TRANSGENIC MOUSE; ULTRASTRUCTURE;

ANIMALS; BASE SEQUENCE; CHROMOSOMES; CHROMOSOMES, ARTIFICIAL, BACTERIAL; DNA, RECOMBINANT; MICE; MICE, INBRED C57BL; MICE, INBRED CBA; MICE, TRANSGENIC; MICROINJECTIONS; MILK PROTEINS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; SEQUENCE HOMOLOGY, NUCLEIC ACID; SWINE;

ANIMALIA; BACTERIA (MICROORGANISMS); MUS MUSCULUS; SUIDAE;

EID: 77956469982     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11248-010-9368-7     Document Type: Article

References (21)

1. Abrahams BS, Chong ACO, Nisha M, Milette D, Brewster DA, Berry ML, Muratkhodjaev F, Mai S, Rajcan-Separovic E, Simpson EM (2003) Metaphase FISHing of transgenic mice recommended: FISH and SKY define BAC-mediated balanced translocation. Genesis 36: 134-141.
2. Bishop J (1996) Chromosomal insertion of foreign DNA. Reprod Nutr Dev 36: 607-618.
3. Chandler KJ, Chandler RL, Broeckelmann EM, Hou Y, Southard-Smith EM, Mortlock DP (2007) Relevance of BAC transgene copy number in mice: transgene copy number variation across multiple transgenic lines and correlations with transgene integrity and expression. Mamm Genome 18: 693-708.
4. Chen C-M, Choo K-B, Cheng W (1995) Frequent deletions and sequence aberrations at the transgene junctions of transgenic mice carrying the papillomavirus regulatory and the SV40 TAg gene sequences. Transgenic Res 4: 52-59.
5. Giraldo P, Rival-Gervier S, Houdebine L-M, Montoliu L (2003) The potential benefits of insulators on heterologous constructs in transgenic animals. Transgenic Res 12: 751-755.
6. Houdebine LM (2007) Transgenic animal models in biomedical research. Methods Mol Biol 360: 163-202.
7. Houdebine LM (2009) Design of expression cassettes for the generation of transgenic animals (including insulators). In: I Anegon (ed) Rat genomics: methods and protocols. Methods in molecular biology, vol 597. doi: 10. 1007/978-1-60327-389-3_4, Humana Press, Totowa, LLC2010.
8. Kamisugi Y, Schlink K, Rensing SA, Schween G, von Stackelberg M, Cuming AC, Reski R, Cove DJ (2006) The mechanism of gene targeting in Physcomitrella patens: homologous recombination, concatenation and multiple integration. Nucleic Acids Res 34: 6205-6214.
9. Kang Y-K, Park JS, Lee C-S, Yeom YI, Chung A-S, Lee K-K (1999) Efficient integration of short interspersed element-flanked foreign DNA via homologous recombination. J Biol Chem 274: 36585-36591.
10. Liang Z, Breman A, Grimes B, Rosen E (2008) Identifying and genotyping transgene integration loci. Transgenic Res 17: 979-983.
11. Liu Y-G, Chen Y (2007) High-efficiency thermal asymmetric interlaced PCR for amplification of unknown flanking sequences. BioTechniques 43: 649-656.
12. Liu Y, Whittier R (1995) Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking. Genomics 25: 674-681.
13. Liu Y-G, Mitsukawa N, Oosumi T, Whittier R (1995) Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. Plant J 8: 457-463.
14. Mehta AK, Majumdar SS, Alam P, Gulati N, Brahmachari V (2009) Epigenetic regulation of cytomegalovirus major immediate-early promoter activity in transgenic mice. Gene 428: 20-24.
15. Montoliu L, Roy R, Regales L, GarcÌa-DÌaz N (2009) Design of vectors for transgene expression: The use of genomic comparative approaches. Comp Immunol Microbiol Infect Dis 32: 81-90.
16. Ochman H, Gerber AS, Hartl DL (1988) Genetic applications of an inverse polymerase chain reaction. Genetics 120: 621-623.
17. Pawlik K, Sun C-W, Higgins P, Townes T (1995) End joining of genomic DNA and transgene DNA in fertilized mouse eggs. Gene 165: 173-181.
18. Saidi S, Rival-Gervier S, Daniel-Carlier N, Thépot D, Morgenthaler C, Viglietta C, Prince S, Passet B, Houdebine L, Jolivet G (2007) Distal control of the pig whey acidic protein (WAP) locus in transgenic mice. Gene 401: 97-107.
19. Tadege M, Wen J, He J, Tu H, Kwak Y, Eschstruth A, Cayrel A, Endre G, Zhao P, Chabaud M, Ratet P, Kirankumar S (2008) Large-scale insertional mutagenesis using the Tnt1 retrotransposon in the model legume Medicago truncatula. Plant J 54: 335-347.
20. VanKeuren M, Gavrilina G, Filipiak W, Zeidler M, Saunders T (2009) Generating transgenic mice from bacterial artificial chromosomes: transgenesis efficiency, integration and expression outcomes Transgenic Res (in press).
21. Würtele H, Little K, Chartrand P (2003) Illegitimate DNA integration in mammalian cells. Gene Ther 10: 1791-1799.