Expression and characterization of human tissue-plasminogen activator in transgenic tobacco plants

Plant Molecular Biology Reporter

Volumn 27, Issue 2, 2009, Pages 209-216

  Hahn, Bum Soo ^a   Sim, Joon Soo ^a   Kim, Hyeong Mi ^a   Ahn, Mi Young ^a   Pak, Hyo Kyung ^a   Kim, Nan A ^a   Kim, Yong Hwan ^a  

^a National Institute of Animal Science   (South Korea)

Author keywords

Fibrin; Human tissue plasminogen activator; Phosphinothricin; Thrombolytic enzyme; Transgenic tobacco

Indexed keywords

CAULIFLOWER MOSAIC VIRUS; MAMMALIA; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM; TOBACCO ETCH VIRUS;

EID: 77956431961     PISSN: 07359640     EISSN: 15729818     Source Type: Journal    
DOI: 10.1007/s11105-008-0075-y     Document Type: Article

References (38)

1. Almquist KC, McLean MD, Niu Y, Byrne G, Olea-Popelka FC, Murrant C, et al. Expression of an anti-botulinum toxin A neutralizing singlechain Fv recombinant antibody in transgenic tobacco. Vaccine 2006;24:2079-86. doi:10.1016/j.vaccine.2005.11.014.
2. Andreasen PA, Riccio A, Welinder KG, Douglas R, Sartorio R, Nielsen LS, et al. Plasminogen activator inhibitor type-1: reactive center and amino-terminal heterogeneity determined by protein and cDNA sequencing. FEBS Lett. 1986;209:213-8. doi:10.1016/0014-5793(86)81113-9.
3. Astrup T, Mullertz S. The fibrin plate method for estimating of fibrinolytic activity. Arch Biochem Biophys. 1952;40:346-51. doi:10.1016/0003-9861(52)90121-5.
4. Barta A, Sommergruber K, Thompson D, Hartmuth K, Matzke MA, Matzke AJM. The expression of a Nopaline synthase human growth hormone chimeric gene in transformed tobacco and sunflower callus tissue. Plant Mol Biol. 1986;6:347-57. doi:10.1007/BF00034942.
5. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem. 1976;72:248-54. doi:10.1016/0003-2697(76)90527-3.
6. Cartwright T. Production of tPA from animal cell culture. In: Spier RE, Griffiths JB, editors. Animal cell biotechnology. London, UK: Academic Press; 1992. p. 218-245.
7. Chong DK, Langridge WH. Expression of full length bioactive antimicrobial human lactoferrin in potato plants. Transgenic Res. 2000;9:71-8. doi:10.1023/A:1008977630179.
8. Collen D, Bounameaux H, DeCock F, Lijnen HR, Verstraete M. Analysis of coagulation and fibrinolysis during intravenous infusion of recombinant human tissue-type plasminogen activator in patients with acute myocardial infarction. Circulation 1986;73:511-7.
9. Collen D, Lijnen HR. Tissue-type plasminogen activator: a historical perspective and personal account. J Thromb Haemost. 2004;2:541-6. doi:10.1111/j.1538-7933.2004.00645.x.
10. Cramer CL, Boothe JG, Oishi KK. Transgenic plants for therapeutic proteins: linking upstream and downstream technologies. Curr Top Microbiol Immunol. 1999;240:95-118.
11. Ding SH, Huang LY, Wang YD, Sun HC, Xiang ZH. High-level expression of basic fibroblast growth factor in transgenic soybean seeds and characterization of its biological activity. Biotechnol Lett. 2006;28:869-75. doi:10.1007/s10529-006-9018-6.
12. Ebert KM, Selgrath JP, DiTullio P, Denman J, Smith TE, Memon MA, et al. Transgenic production of a variant of human tissue-type plasminogen activator in goat milk: generation of transgenic goats and analysis of expression. Biotechnology (N Y). 1991;9:835-8. doi:10.1038/nbt0991-835.
13. Galeffi P, Lombardi A, Donato MD, Latini A, Sperandei M, Cantale C, et al. Expression of single-chain antibodies in transgenic plants. Vaccine 2005;23:1823-7. doi:10.1016/j.vaccine.2004.11.025.
14. Horsch RB, Fry J, Hoffmann N, Neidermeyer J, Ogers SG, Fraley RT. Leaf disc transformation. Plant Mol Biol Mann. 1998; A5:1-9.
15. Jarvis DL, Summers MD. Glycosylation and secretion of human tissue plasminogen activator in recombinant baculovirus-infected insect cells. Mol Cell Biol. 1989;9:214-23.
16. Kausch AP, Owen TP Jr, Zachwieja SJ, Flynn AR, Sheen J. Mesophyll-specific, light and metabolic regulation of the C4 PPCZm1 promoter in transgenic maize. Plant Mol Biol. 2001;45:1-15. doi:10.1023/A:1006487326533.
17. Khoudi H, Laberge S, Ferullo JM, Bazin R, Darveau A, Castonguay Y, et al. Production of a diagnostic monoclonal antibody in perennial alfalfa plants. Biotechnol Bioeng. 1999;64:135-43. doi:10.1002/(SICI)1097-0290(19990720)64:2<135::AID-BIT2>3.0.CO;2-Q .
18. Kim JY, Fogarty EA, Lu FJ, Zhu H, Wheelock GD, Henderson LA, et al. Twin-arginine translocation of active human tissue plasminogen activator in Escherichia coli. Appl Environ Microbiol. 2005;71:8451-9. doi:10.1128/AEM.71.12.8451-8459.2005.
19. Kusnadi A, Nikolov ZL, Howard JA. Production of recombinant proteins in transgenic plants: practical considerations. Biotechnol Bioeng. 1997;56:473-84. doi:10.1002/(SICI)1097-0290 (19971205)56:5<473::AID-BIT1>3.0.CO;2-F.
20. Ma JK, Hikmat BY, Wycoff K, Vine ND, Chargelegue D, Yu L, et al. Characterization of a recombinant plant monoclonal secretory antibody and preventive immunotherapy in humans. Nat Med. 1998;4:601-6. doi:10.1038/nm0598-601.
21. Ma JK, Drake PM, Christou P. The production of recombinant pharmaceutical proteins in plants. Nat Rev Genet. 2003;4:794-805. doi:10.1038/nrg1177.
22. Manosroi J, Tayapiwatana C, Gotz F, Werner RG, Manosroi A. Secretion of active recombinant human tissue plasminogen activator derivatives in Escherichia coli. Appl Environ Microbiol. 2001;67:2657-64. doi:10.1128/AEM.67.6.2657-2664.2001.
23. McCormick AA, Kumagai MH, Hanley K, Turpen TH, Hakim I, Grill LK, et al. Rapid production of specific vaccines for lymphoma by expression of the tumor-derived single-chain Fv epitopes in tobacco plants. Proc Natl Acad Sci USA. 1999;96:703-8. doi:10.1073/pnas.96.2.703.
24. Outchkourov NS, Rogelj B, Strukelj B, Jongsma MA. Expression of sea anemone equistatin in potato. Effects of plant proteases on heterologous protein production. Plant Physiol. 2003;133:379-90. doi:10.1104/pp.102.017293.
25. Pennica D, Holmes WE, Kohr WJ, Harkins RN, Vehar GA, Ward CA, et al. Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli. Nature 1983;301:214-21. doi:10.1038/301214a0.
26. Pittius CW, Hennighausen L, Lee E, Westphal H, Nicols E, Vitale J, et al. A milk protein gene promoter directs the expression of human tissue plasminogen activator cDNA to the mammary gland in transgenic mice. Proc Natl Acad Sci USA. 1998;85:5874-8. doi:10.1073/pnas.85.16.5874.
27. Qiu J, Swartz JR, Georgiou G. Expression of active human tissue-type plasminogen activator in Escherichia coli. Appl Environ Microbiol. 1998;64:4891-6.
28. Reddy VB, Garramone AJ, Sasak H, Wei CM, Watkins P, Galli J, et al. Expression of human uterine tissue-type plasminogen activator inmouse cells using BPV vectors. DNA 1987;6:461-72.
29. Ruggiero F, Exposito JY, Bournat P, Gruber V, Perret S, Comte J, et al. Triple helix assembly and processing of human collagen produced in transgenic tobacco plants. FEBS Lett. 2000;469:132-6. doi:10.1016/S0014-5793(00)01259-X.
30. Schinkel H, Schiermeyer A, Soeur R, Fischer R, Schillberg S. Production of an active recombinant thrombomodulin derivative in transgenic tobacco plants and suspension cells. Transgenic Res. 2005;14:251-9.
31. Sharp JM, Doran PM. Characterization of monoclonal antibody fragments produced by plant cells. Biotechnol Bioeng. 2001;73:338-46. doi:10.1002/bit.1067.
32. Stevens LH, Stoopen GM, Elbers IJ, Molthoff JW, Bakker HA, Lommen A, et al. Effect of climate conditions and plant developmental stage on the stability of antibodies expressed in transgenic tobacco. Plant Physiol. 2000;124:173-82. doi:10.1104/pp.124.1.173.
33. Stoger E, Vaquero C, Torres E, Sack M, Nicholson L, Drossard J, et al. Cereal crops as viable production and storage systems for pharmaceutical scFv antibodies. Plant Mol Biol. 2000;42:583-90. doi:10.1023/A:1006301519427.
34. Stoger E, Ma JK, Fischer R, Christou P. Sowing the seeds of success: pharmaceutical proteins from plants. Curr Opin Biotechnol. 2005;16:167-73. doi:10.1016/j.copbio.2005.01.005.
35. Torres E, Vaquero C, Nicholson L, Sack M, Stoger E, Drossard J, et al. Rice cell culture as an alternative production system for functional diagnostic and therapeutic antibodies. Transgenic Res. 1999;8:441-9. doi:10.1023/A:1008969031219.
36. Upshall A, Kumar AA, Bailey MC, Parker MD, Favreau MA. Secretion of active human tissue plasminogen activator from the filamentous fungus Aspergillus nidulans. Bio/Technology. 1987;5:1301-4. doi:10.1038/nbt1287-1301.
37. Vaquero C, Sack M, Chandler J, Drossard J, Schuster F, Monecke M, et al. Transient expression of a tumor-specific single-chain fragment and a chimeric antibody in tobacco leaves. Proc Natl Acad Sci USA. 1999;96:11128-33. doi:10.1073/pnas.96.20.11128.
38. Wang ML, Goldstein C, Su W, Moore PH, Albert HH. Production of biologically active GM-CSF in sugarcane: a secure biofactory. Transgenic Res. 2005;14:167-78. doi:10.1007/s11248-004-5415-6.