Immunofluorescence analysis of baculovirus-displayed viral proteins on infected insect cells

Cold Spring Harbor Protocols

Volumn 5, Issue 3, 2010, Pages

  Mäkelä, Anna R ^a   Ernst, Wolfgang ^a   Grabherr, Reingard ^a   Oker Blom, Christian ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

BACULOVIRUS VECTOR; VIRUS PROTEIN;

ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; CELL GROWTH; CLINICAL PROTOCOL; CONFOCAL MICROSCOPY; CULTURE TECHNIQUE; CYTOLOGY; FLUORESCENCE MICROSCOPY; GENE LIBRARY; IMMUNOFLUORESCENCE TEST; INSECT CELL; INSECT CELL CULTURE; INSECT DISEASE; INSECT GENETICS; LEPIDOPTERA; NONHUMAN; PHAGE DISPLAY; PROCESS DESIGN; VIRUS RECOMBINANT; WILD TYPE;

HEXAPODA;

EID: 77956291973     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5395     Document Type: Article

References (8)

1. Mäkelä AR, Ernst W, Grabherr R, Oker-Blom C. 2010a. Immunoelectron microscopy analysis of recombinant baculovirus display viruses. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5396.
2. Mäkelä AR, Ernst W, Grabherr R, Oker-Blom C. 2010b. Baculovirus-based display and gene delivery systems. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.top72.
3. Mäkelä AR, Ernst W, Grabherr R, Oker-Blom C. 2010c. Creation of baculovirus display libraries. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5393.
4. Mäkelä AR, Ernst W, Grabherr R, Oker-Blom C. 2010d. Determination of recombinant baculovirus display viral titer. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5394
5. Mäkelä AR, Ernst W, Grabherr R, Oker-Blom C. 2010e. Monitoring baculovirus-mediated efficiency of gene delivery. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5397
6. Ojala K, Mottershead DG, Suokko A, Oker-Blom C. 2001. Specific binding of baculoviruses displaying gp64 fusion proteins to mammalian cells. Biochem Biophys Res Commun 284: 777-784.
7. Ojala K, Koski J, Ernst W, Grabherr R, Jones I, Oker-Blom C. 2004. Improved display of synthetic IgG-binding domains on the baculovirus surface. Technol Cancer Res Treat 3: 77-84.
8. Toivola J, Ojala K, Michel PO, Vuento M, Oker-Blom C. 2002. Properties of baculovirus particles displaying GFP analyzed by fluorescence correlation spectroscopy. Biol Chem 383: 1941-1946.