Determination of recombinant baculovirus display viral titer

Cold Spring Harbor Protocols

Volumn 5, Issue 3, 2010, Pages

  Mäkelä, Anna R ^a   Ernst, Wolfgang ^a   Grabherr, Reingard ^a   Oker Blom, Christian ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

BACULOVIRUS VECTOR; BETA GALACTOSIDASE; GREEN FLUORESCENT PROTEIN;

ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; CELL GROWTH; CLINICAL PROTOCOL; CULTURE TECHNIQUE; IMMUNOASSAY; INSECT CELL CULTURE; INSECT GENETICS; LEPIDOPTERA; MARKER GENE; MICROSCOPY; NONHUMAN; PHAGE DISPLAY; PROMOTER REGION; VIRUS RECOMBINANT; VIRUS TITRATION;

EID: 77956282807     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5394     Document Type: Article

References (10)

1. BD Biosciences Clontech. 2003. BacPAK baculovirus rapid titer kit user manual. Clontech, Mountain View, CA.
2. King LA, Possee RD. 1992. The baculovirus expression system, 1st ed. Chapman & Hall, London.
3. Mäkelä AR, Ernst W, Grabherr R, Oker-Blom C. 2010a. Baculovirus-based display and gene delivery systems. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.top72.
4. Mäkelä AR, Ernst W, Grabherr R, Oker-Blom C. 2010b. Creation of baculovirus display libraries. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5393.
5. Mäkelä AR, Ernst W, Grabherr R, Oker-Blom C. 2010c. Immunofluorescence analysis of baculovirus-displayed viral proteins on infected insect cells. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5395.
6. Mäkelä AR, Ernst W, Grabherr R, Oker-Blom C. 2010d. Immunoelectron microscopy analysis of recombinant baculovirus display viruses. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5396.
7. Mäkelä AR, Ernst W, Grabherr R, Oker-Blom C. 2010e. Monitoring baculovirus-mediated efficiency of gene delivery. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5397.
8. O'Reilly DR, Miller LK, Luckow VA. 1994. Baculovirus expression vectors: A laboratory manual. Oxford University Press, New York.
9. Summers MD, Smith GE. 1987. A manual of methods for baculovirus vectors and insect cell culture procedures, 2nd ed. Texas Agricultural Experiment Station, College Station, TX.
10. Volkman LE, Goldsmith PA. 1982. Generalized immunoassay for Autographa californica nuclear polyhedrosis virus infectivity in vitro. Appl Environ Microbiol 44: 227-233.