Monitoring baculovirus-mediated efficiency of gene delivery

Cold Spring Harbor Protocols

Volumn 5, Issue 3, 2010, Pages

  Mäkelä, Anna R ^a   Ernst, Wolfgang ^a   Grabherr, Reingard ^a   Oker Blom, Christian ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

BACULOVIRUS VECTOR; LUCIFERASE;

ARTICLE; BACULOVIRUS; BACULOVIRUS EXPRESSION SYSTEM; CLINICAL EFFECTIVENESS; CLINICAL PROTOCOL; ENZYME ACTIVITY; HUMAN; HUMAN CELL CULTURE; INCUBATION TIME; NONHUMAN; PROCESS DESIGN; PROCESS MONITORING; SENSITIVITY AND SPECIFICITY; TRANSGENE; VIRAL GENE DELIVERY SYSTEM; VIRUS RECOMBINANT; VIRUS TITRATION;

EID: 77956284970     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5397     Document Type: Article

References (9)

1. King LA, Possee RD. 1992. The baculovirus expression system, 1st ed. Chapman & Hall, London.
2. Lo H-R, Chao Y-C. 2004. Rapid titer determination of baculovirus by quantitative real-time polymerase chain reaction. Biotechnol Prog 20: 354-360.
3. Mäkelä AR, Ernst W, Grabherr R, Oker-Blom C. 2010a. Baculovirus-based display and gene delivery systems. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.top72.
4. Mäkelä AR, Ernst W, Grabherr R, Oker-Blom C. 2010b. Creation of baculovirus display libraries. Cold Spring Harb Protoc (this issue) doi: 10.1101/pdb.prot5393.
5. Mäkelä AR, Ernst W, Grabherr R, Oker-Blom C. 2010c. Determination of recombinant baculovirus display viral titer. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5394.
6. Mäkelä AR, Ernst W, Grabherr R, Oker-Blom C. 2010d. Immunofluorescence analysis of baculovirus-displayed viral proteins on infected insect cells. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5395.
7. Mäkelä AR, Ernst W, Grabherr R, Oker-Blom C. 2010e. Immunoelectron microscopy analysis of recombinant baculovirus display viruses. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5396.
8. O'Reilly DR, Miller LK, Luckow VA. 1994. Baculovirus expression vectors: A laboratory manual. Oxford University Press, New York.
9. Summers MD, Smith GE. 1987. A manual of methods for baculovirus vectors and insect cell culture procedures, 2nd ed. Texas Agricultural Experiment Station, College Station, TX.