Imaging single molecular motor motility with total internal reflection fluorescence microscopy (TIRFM)

Cold Spring Harbor Protocols

Volumn 5, Issue 3, 2010, Pages

  Reck Peterson, Samara L ^a   Derr, Nathan D ^a   Stuurman, Nico ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; DYNEIN ADENOSINE TRIPHOSPHATASE; MOLECULAR MOTOR;

ARTICLE; AXONEME; BIOASSAY; CLINICAL PROTOCOL; CYTOPLASM; ENZYME PURIFICATION; FLUORESCENCE MICROSCOPY; MICROTUBULE ASSEMBLY; MOLECULAR DYNAMICS; MOLECULAR IMAGING; MOTOR ACTIVITY; MOTOR UNIT; NONHUMAN; PROCESS DESIGN; SACCHAROMYCES CEREVISIAE; TOTAL INTERNAL REFLECTION FLUORESCENCE MICROSCOPY;

EID: 77956278998     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5399     Document Type: Article

References (5)

1. Gibbons IR, Fronk E. 1979. A latent adenosine triphosphatase form of dynein 1 from sea urchin sperm flagella. J Biol Chem 254: 187-196.
2. Kapitein LC, Kwok BH, Weinger JS, Schmidt CF, Kapoor TM, Peterman EJ. 2008. Microtubule cross-linking triggers the directional motility of kinesin-5. J Cell Biol 182: 421-428.
3. Reck-Peterson SL, Derr ND, Stuurman N. 2010a. Imaging single molecules using total internal reflection fluorescence microscopy (TIRFM). Cold Spring Harb Protoc (this issue). doi: 10.1011/pdb.top73.
4. Reck-Peterson SL, Derr ND, Stuurman N. 2010b. Determining single-molecule intensity as a function of power density. Cold Spring Harb Protoc (this issue). doi: 10.1011/pdb.prot5398.
5. Yildiz A, Forkey JN, McKinney SA, Ha T, Goldman YE, Selvin PR. 2003. Myosin V walks hand-over-hand: Single fluorophore imaging with 1.5-nm localization. Science 300: 2061-2065