Synthesis of human renalase1 in Escherichia coli and its purification as a FAD-containing holoprotein

Protein Expression and Purification

Volumn 72, Issue 2, 2010, Pages 244-253

  Pandini, Vittorio ^a   Ciriello, Francesco ^a   Tedeschi, Gabriella ^a   Rossoni, Giuseppe ^a   Zanetti, Giuliana ^a   Aliverti, Alessandro ^a  

^a UNIVERSITY OF MILAN   (Italy)

Author keywords

Circular dichroism; Flavin; Flavoprotein; Hypertension; Immobilized metal affinity chromatography; Mass spectrometry; Plasma protein; SUMO protein

Indexed keywords

ESCHERICHIA COLI; VERTEBRATA;

AMINE OXIDASE (FLAVIN CONTAINING); FLAVINE ADENINE NUCLEOTIDE; HISTIDINE; POLYHISTIDINE; RECOMBINANT PROTEIN; RENALASE; SERINE; SUMO 1 PROTEIN;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BIOSYNTHESIS; CHEMISTRY; CIRCULAR DICHROISM; DRUG EFFECT; ESCHERICHIA COLI; GENETICS; HEMODYNAMICS; HUMAN; ISOLATION AND PURIFICATION; MALE; MASS SPECTROMETRY; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; RAT; SPRAGUE DAWLEY RAT;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CHROMATOGRAPHY, AFFINITY; CIRCULAR DICHROISM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; FLAVIN-ADENINE DINUCLEOTIDE; HEMODYNAMICS; HISTIDINE; HUMANS; MALE; MOLECULAR SEQUENCE DATA; MONOAMINE OXIDASE; RATS; RATS, SPRAGUE-DAWLEY; RECOMBINANT PROTEINS; SERINE; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SUMO-1 PROTEIN;

EID: 77956196860     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.03.008     Document Type: Article

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