Cloning, expression, and purification of choline dehydrogenase from the moderate halophile Halomonas elongata

Applied and Environmental Microbiology

Volumn 69, Issue 4, 2003, Pages 2126-2132

  Gadda, Giovanni ^a   McAllister Wilkins, Elien Elizabeth ^a  

^a GEORGIA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; CLONING; ELECTROPHORESIS; ENZYMES; ESCHERICHIA COLI; OXIDATION;

ENVIRONMENTAL STRESSES;

MICROBIOLOGY;

2 DIETHYLAMINOETHANOL; ALDEHYDE; AMMONIUM SULFATE; BACTERIAL ENZYME; BETAINE; CHOLINE; CHOLINE DEHYDROGENASE; DODECYL SULFATE SODIUM; GENE PRODUCT; GLYCINE; GLYCINE CLEAVAGE SYSTEM; OXIDOREDUCTASE; OXYGEN; PHENAZINE METHOSULFATE; POLYACRYLAMIDE GEL; PROTEIN BETA; RECOMBINANT ENZYME; SEPHAROSE; UNCLASSIFIED DRUG;

ENZYME; MICROBIOLOGY;

ADAPTATION; ARTICLE; BETA GENE; BIOCHEMISTRY; BIOTECHNOLOGY; COLUMN CHROMATOGRAPHY; ELECTRON TRANSPORT; ENZYME ANALYSIS; ENZYME ENGINEERING; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; FREEZING; GENE ISOLATION; HALOMONAS; HALOMONAS ELONGATA; HALOPHILIC BACTERIUM; HIGH TEMPERATURE PROCEDURES; IN VITRO STUDY; MICHAELIS CONSTANT; MOLECULAR CLONING; MOLECULAR DYNAMICS; NONHUMAN; OXIDATION KINETICS; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; REACTION ANALYSIS; SALINITY; STRESS; TRANSGENIC PLANT;

ALCOHOL OXIDOREDUCTASES; BACTERIAL PROTEINS; BETAINE; CHOLINE; CHOLINE DEHYDROGENASE; CLONING, MOLECULAR; ESCHERICHIA COLI; HALOMONAS; KINETICS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; HALOMONAS; HALOMONAS ELONGATA; NEGIBACTERIA;

EID: 0037391235     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.69.4.2126-2132.2003     Document Type: Article

References (48)

1. Alia, H. Hayashi, T. H. H. Chen, and N. Murata. 1998. Transformation with a gene for choline oxidase enhances cold tolerance of Arabidopsis during germination and early growth. Plant Cell Environ. 21:232-239.
2. Alia, H. Hayashi, A. Sakamoto, and N. Murata. 1998. Enhancement of the tolerance of Arabidopsis to high temperatures by genetic engineering of the synthesis of glycinebetaine. Plant J. 16:155-161.
3. Andresen, P. A., I. Kaasen, O. B. Styrvold, G. Boulnois, and A. R. Strøm. 1988. Molecular cloning, physical mapping and expression of the bet genes governing the osmoregulatory choline-glycine betaine pathway of Escherichia coli. J. Gen. Microbiol. 134:1737-1746.
4. Bell, R. P. 1966. The reversible hydration of carbonyl compounds. Adv. Phys. Org. Chem. 4:1-29.
5. Boch, J., G. Nau-Wagner, S. Kneip, and E. Bremer. 1997. Glycine betaine aldehyde dehydrogenase from Bacillus subtilis: characterization of an enzyme required for the synthesis of the osmoprotectant glycine betaine. Arch. Microbiol. 168:282-289.
6. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
7. Bremer, E., and R. Kramer. 2000. Coping with osmotic challenges: osmoregulation through accumulation and release of compatible solutes in bacteria, p. 79-97. In G. Storz and R. Hengge-Areonis (ed.), Bacterial stress response. ASM Press, Washington, D.C.
8. Canovas, D., C. Vargas, L. N. Csonka, A. Ventosa, and J. J. Nieto. 1998. Synthesis of glycine betaine from exogenous choline in the moderately halophilic bacterium Halomonas elongota. Appl. Environ. Microbiol. 64:4095-4097.
9. Canovas, D., C. Vargas, S. Kneip, M. J. Moron, A. Ventosa, E. Bremer, and J. J. Nieto. 2000. Genes for the synthesis of the osmoprotectant glycine betaine from choline in the moderately halophilic bacterium Halomonas elongata DSM 3043. Microbiology 146:455-463.
10. Cavener, D. R. 1992. GMC oxidoreductases. A newly defined family of homologous proteins with diverse catalytic activities. J. Mol. Biol. 223:811-814.
11. Deshnium, P., D. A. Los, H. Hayashi, L. Mustardy, and N. Murata. 1995. Transformation of Synechococcus with a gene for choline oxidase enhances tolerance to salt stress. Plant Mol. Biol. 29:897-907.
12. Farwick, M., R. M. Siewe, and R. Kramer. 1995. Glycine betaine uptake after hyperosmotic shift in Corynebacterium glutamicum. J. Bacteriol. 177:4690-4695.
13. Gerhardt, P. N., L. T. Smith, and G. M. Smith. 1996. Sodium-driven, osmotically activated glycine betaine transport in Listeria monocytogenes membrane vesicles. J. Bacteriol. 178:6105-6109.
14. Gerhardt, P. N., L. Tombras Smith, and G. M. Smith. 2000. Osmotic and chill activation of glycine betaine porter II in Listeria monocytogenes membrane vesicles. J. Bacteriol. 182:2544-2550.
15. Hanson, A. D., B. Rathinasabapathi, J. Rivoal, M. Burnet, M. O. Dillon, and D. A. Gage. 1994. Osmoprotective compounds in the Plumbaginaceae: a natural experiment in metabolic engineering of stress tolerance. Proc. Natl. Acad. Sci. USA 91:306-310.
16. Hayashi, H., Alia, L. Mustardy, P. Deshnium, M. Ida, and N. Murata. 1997. Transformation of Arabidopsis thaliana with the codA gene for choline oxidase; accumulation of glycinebetaine and enhanced tolerance to salt and cold stress. Plant J. 12:133-142.
17. Hayashi, H., Alia, A. Sakamoto, H. Nonaka, T. H. H. Chen, and N. Murata. 1998. Enhanced germination under high-salt conditions of seeds of transgenic Arabidopsis with a bacterial gene (codA) for choline oxidase. J. Plant Res. 111:357-362.
18. Holmström, K. O., S. Somersalo, A. Mandal, T. E. Palva, and B. Welin. 2000. Improved tolerance to salinity and low temperature in transgenic tobacco producing glycine betaine. J. Exp. Bot. 51:177-185.
19. Ikuta, S., S. Imamura, H. Misaki, and Y. Horiuti. 1977. Purification and characterization of choline oxidase from Arthrobacter globiformis. J. Biochem. (Tokyo) 82:1741-1749.
20. Inoue, H., H. Nojima, and H. Okayama. 1990. High efficiency transformation of Escherichia coli with plasmids. Gene 96:23-28.
21. Ishitani, M., T. Nakamura, S. Y. Han, and T. Takabe. 1995. Expression of the betaine aldehyde dehydrogenase gene in barley in response to osmotic stress and abscisic acid. Plant Mol. Biol. 27:307-315.
22. Kappes, R. M., B. Kempf, and E. Bremer. 1996. Three transport systems for the osmoprotectant glycine betaine operate in Bacillus subtilis: characterization of OpuD. J. Bacteriol. 178:5071-5079.
23. Kempf, B., and E. Bremer. 1998. Uptake and synthesis of compatible solutes as microbial stress responses to high-osmolality environments. Arch. Microbiol. 170:319-330.
24. Ko, R., and L. T. Smith. 1999. Identification of an ATP-driven, osmoregulated glycine betaine transport system in Listeria monocytogenes. Appl. Environ. Microbiol. 65:4040-4048.
25. Kunkel, T. A. 1985. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA 82:488-492.
26. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
27. Landfald, B., and A. R. Strøm. 1986. Choline-glycine betaine pathway confers a high level of osmotic tolerance in Escherichia coli. J. Bacteriol. 165:849-855.
28. Lartillot, S. 1987. A simplified method of production of choline oxidase suitable for choline assay. Prep. Biochem. 17:283-295.
29. Lever, M., P. C. Sizeland, L. M. Bason, C. M. Hayman, and S. T. Chambers. 1994. Glycine betaine and proline betaine in human blood and urine. Biochim. Biophys. Acta 1200:259-264.
30. Mendum, M. L., and L. T. Smith. 2002. Characterization of glycine betaine porter I from Listeria monocytogenes and its roles in salt and chill tolerance. Appl. Environ. Microbiol. 68:813-819.
31. Perrino, L. A., and S. K. Pierce. 2000. Betaine aldehyde dehydrogenase kinetics partially account for oyster population differences in glycine betaine synthesis. J. Exp. Zool. 286:238-249.
32. Perrino, L. A., and S. K. Pierce. 2000. Choline dehydrogenase kinetics contribute to glycine betaine regulation differences in Chesapeake Bay and Atlantic oysters. J. Exp. Zool. 286:250-261.
33. Peter, H., A. Burkovski, and R. Kramer. 1996. Isolation, characterization, and expression of the Corynebacterium glutamicum betP gene, encoding the transport system for the compatible solute glycine betaine. J. Bacteriol. 178:5229-5234.
34. Pocard, J. A., N. Vincent, E. Boncompagni, L. T. Smith, M. C. Poggi, and D. Le Rudulier. 1997. Molecular characterization of the bet genes encoding glycine betaine synthesis in Sinorhizobium meliloti 102F34. Microbiology 143:1369-1379.
35. Randall, K., M. Lever, B. A. Peddie, and S. T. Chambers. 1996. Accumulation of natural and synthetic betaines by a mammalian renal cell line. Biochem. Cell Biol. 74:283-287.
36. Rathinasabapathi, B., M. Burnet, B. L. Russell, D. A. Gage, P. C. Liao, G. J. Nye, P. Scott, J. H. Golbeck, and A. D. Hanson. 1997. Choline monooxygenase, an unusual iron-sulfur enzyme catalyzing the first step of glycine betaine synthesis in plants: prosthetic group characterization and cDNA cloning, Proc. Natl. Acad. Sci. USA 94:3454-3458.
37. Rozwadowski, K. L., G. G. Khachatourians, and G. Selvaraj. 1991. Choline oxidase, a catabolic enzyme in Arthrobacter pascens, facilitates adaptation to osmotic stress in Escherichia coli. J. Bacteriol. 173:472-478.
38. Russell, B. L., B. Rathinasabapathi, and A. D. Hanson. 1998. Osmotic stress induces expression of choline monooxygenase in sugar beet and amaranth. Plant Physiol. 116:859-865.
39. Russell, R., and R. K. Scopes. 1994. Use of hydrophobic chromatography for purification of the membrane-located choline dehydrogenase from a Pseudomonas strain. Bioseparation 4:279-284.
40. Sakamoto, A., R. Valverde, Alia, T. H. Chen, and N. Murata. 2000. Transformation of Arabidopsis with the codA gene for choline oxidase enhances freezing tolerance of plants. Plant J. 22:449-453.
41. Sizeland, P. C., S. T. Chambers, M. Lever, L. M. Bason, and R. A. Robson. 1993. Organic osmolytes in human and other mammalian kidneys. Kidney Int. 43:448-453.
42. Styrvold, O. B., P. Falkenberg, B. Landfald, M. W. Eshoo, T. Bjornsen, and A. R. Strøm. 1986. Selection, mapping, and characterization of osmoregulatory mutants of Escherichia coli blocked in the choline-glycine betaine pathway. J. Bacteriol. 165:856-863.
43. Subbarao, G. V., R. M. Wheeler, L. H. Levine, and G. W. Stutte. 2001. Glycine betaine accumulation, ionic and water relations of red-beet at contrasting levels of sodium supply. J. Plant Physiol. 158:767-776.
44. Tsuge, H., Y. Nakano, H. Onishi, Y. Futamura, and K. Ohashi. 1980. A novel purification and some properties of rat liver mitochondrial choline dehydrogenase. Biochim. Biophys. Acta 614:274-284.
45. Velasco-Garcia, R., C. Mujica-Jimenez, G. Mendoza-Hernandez, and R. A. Munoz-Clares. 1999, Rapid purification and properties of betaine aldehyde dehydrogenase from Pseudomonas aeruginosa. J. Bacteriol. 181:1292-1300.
46. Ventosa, A., J. J. Nieto, and A. Oren. 1998. Biology of moderately halophilic aerobic bacteria. Microbiol. Mol. Biol. Rev. 62:504-544.
47. Yamada, H., N. Mori, and Y. Tani. 1979. Properties of choline oxidase of Cylyndrocarpon didymum M-1. Agric. Biol. Chem. 43:2173-2177.
48. Yancey, P. H. 1988. Osmotic effectors in kidneys of xeric and mesic rodents: corticomedullary distributions and changes with water availability. J. Comp. Physiol. B 158:369-380.