Enhanced specificity of real-time PCR for measurement of hepatitis B virus cccDNA using restriction endonuclease and plasmid-safe ATP-dependent DNase and selective primers

Journal of Virological Methods

Volumn 169, Issue 1, 2010, Pages 181-187

  Gao, Ying Tang ^a   Han, Tao ^a   Li, Ying ^a   Yang, Bin ^a   Wang, Yi Jun ^a   Wang, Feng Mei ^a   Jing, Xiang ^a   Du, Zhi ^a  

^a TIANJIN THIRD CENTRAL HOSPITAL   (China)

Author keywords

CccDNA; Hepatitis B virus; Plasmid safe ATP dependent DNase; Quantitation; Real time PCR; Restriction endonuclease

Indexed keywords

ADENOSINE TRIPHOSPHATE; CIRCULAR DNA; COVALENTLY CLOSED CIRCULAR DNA; DEOXYRIBONUCLEASE; PLASMID DNA; RESTRICTION ENDONUCLEASE; UNCLASSIFIED DRUG; VIRUS DNA;

ACCURACY; ADULT; ARTICLE; BLOOD ANALYSIS; CLINICAL ARTICLE; CONTROLLED STUDY; DNA EXTRACTION; FALSE POSITIVE RESULT; FEMALE; GENE AMPLIFICATION; HEPATITIS B VIRUS; HUMAN; HUMAN TISSUE; LIVER; LIVER CELL CARCINOMA; MALE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RECOMBINANT PLASMID; REPRODUCIBILITY; RESTRICTION MAPPING; SENSITIVITY AND SPECIFICITY;

ADULT; CARCINOMA, HEPATOCELLULAR; DNA PRIMERS; DNA RESTRICTION ENZYMES; DNA, VIRAL; EXODEOXYRIBONUCLEASE V; FEMALE; HEPATITIS B; HEPATITIS B VIRUS; HUMANS; LIVER; LIVER NEOPLASMS; MALE; MIDDLE AGED; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SERUM; VIROLOGY;

HEPATITIS B VIRUS;

EID: 77956185597     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2010.07.031     Document Type: Article

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