Sugaring out: A new method for removal of acetonitrile from preparative RP-HPLC eluent for protein purification

Process Biochemistry

Volumn 45, Issue 10, 2010, Pages 1672-1676

  Dhamole, Pradip B ^a   Mahajan, Prafulla ^a   Feng, Hao ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

Acetonitrile; Liquid liquid extraction; Proteins; Solvent extraction; Sugaring out

Indexed keywords

ACETONITRILE-WATER MIXTURE; BOVINE SERUM ALBUMINS; GLUCOSE CONCENTRATION; LIQUID-LIQUID EXTRACTION; NEAR ROOM TEMPERATURE; OPTIMIZED CONDITIONS; PROTEIN PURIFICATION; PROTEIN RECOVERY; REVERSE PHASE; RP-HPLC; SALTING OUT; SEPARATION METHODS; SUB-ZERO TEMPERATURES; SUGARING-OUT; UNIVERSITY OF ILLINOIS; WATER SOLUBLE PROTEIN;

ACETONITRILE; BODY FLUIDS; EFFLUENTS; GLUCOSE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH PRESSURE LIQUID CHROMATOGRAPHY; LIQUIDS; ORGANIC SOLVENTS; PHASE SEPARATION; PROTEINS; PURIFICATION;

SOLVENT EXTRACTION;

BOVINAE;

EID: 77956180410     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2010.06.020     Document Type: Article

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