Creation and biophysical characterization of a high-affinity, monomeric EGF receptor ectodomain using fluorescent proteins

Biochemistry

Volumn 49, Issue 35, 2010, Pages 7459-7466

  Kozer, Noga ^a,b   Henderson, Christine ^a,b   Bailey, Michael F ^c   Rothacker, Julie ^a   Nice, Edouard C ^a   Burgess, Anthony W ^a   Clayton, Andrew H A ^a,b  

^a ROYAL MELBOURNE HOSPITAL   (Australia)

^b SWINBURNE UNIVERSITY OF TECHNOLOGY   (Australia)

^c UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ANISOTROPY; CENTRIFUGATION; CONFORMATIONS; FLUORESCENCE; MONOCLONAL ANTIBODIES; PROTEINS;

ANALYTICAL ULTRACENTRIFUGATION; BIOPHYSICAL CHARACTERIZATION; C-TERMINUS; CRYSTAL MODELS; ECTODOMAIN; ENHANCED GREEN FLUORESCENT PROTEIN; EPIDERMAL GROWTH FACTOR RECEPTORS; FLUORESCENCE ANISOTROPY; FLUORESCENT PROTEIN; HIGH AFFINITY; LIGAND BINDING; NANOMOLAR RANGE; PROTEIN FLEXIBILITY; RED FLUORESCENT PROTEINS; SHAPE CHANGE; STEADY-STATE ANISOTROPY; X-RAY STRUCTURAL STUDY;

REDUCTION;

EPIDERMAL GROWTH FACTOR; EPIDERMAL GROWTH FACTOR RECEPTOR; GREEN FLUORESCENT PROTEIN; ENHANCED GREEN FLUORESCENT PROTEIN;

AMINO TERMINAL SEQUENCE; ANISOTROPY; ARTICLE; CARBOXY TERMINAL SEQUENCE; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN STRUCTURE; X RAY ANALYSIS; CELL CULTURE; CHEMICAL STRUCTURE; CHEMISTRY; DIMERIZATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENETIC TRANSFECTION; KINETICS; METABOLISM; PROTEIN TERTIARY STRUCTURE;

CELLS, CULTURED; DIMERIZATION; EPIDERMAL GROWTH FACTOR; FLUORESCENCE RESONANCE ENERGY TRANSFER; GREEN FLUORESCENT PROTEINS; HUMANS; KINETICS; MODELS, MOLECULAR; PROTEIN STRUCTURE, TERTIARY; RECEPTOR, EPIDERMAL GROWTH FACTOR; TRANSFECTION;

EID: 77956135048     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi1008134     Document Type: Article

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